scholarly journals Indole Glucosinolate Biosynthesis Limits Phenylpropanoid Accumulation in Arabidopsis thaliana

2015 ◽  
Vol 27 (5) ◽  
pp. 1529-1546 ◽  
Author(s):  
Jeong Im Kim ◽  
Whitney L. Dolan ◽  
Nickolas A. Anderson ◽  
Clint Chapple
Keyword(s):  
Arabidopsis Thaliana ◽  
Glucosinolate Biosynthesis ◽  
Indole Glucosinolate

scholarly journals Methyl Transfer in Glucosinolate Biosynthesis Mediated by Indole Glucosinolate O-Methyltransferase 5

2016 ◽  
Vol 172 (4) ◽  
pp. 2190-2203 ◽  
Author(s):  
Marina Pfalz ◽  
Maisara Mukhaimar ◽  
François Perreau ◽  
Jayne Kirk ◽  
Cecilie Ida Cetti Hansen ◽  
...  
Keyword(s):  
Glucosinolate Biosynthesis ◽  
Methyl Transfer ◽  
Indole Glucosinolate

Indole glucosinolate and auxin biosynthesis in Arabidopsis thaliana (L.) Heynh. glucosinolate mutants and the development of clubroot disease

Planta ◽  
1999 ◽  
Vol 208 (3) ◽  
pp. 409-419 ◽  
Author(s):  
Jutta Ludwig-Müller ◽  
Kerstin Pieper ◽  
Manfred Ruppel ◽  
Jerry D. Cohen ◽  
Ephraim Epstein ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Auxin Biosynthesis ◽  
Clubroot Disease ◽  
Indole Glucosinolate

scholarly journals Conservation and clade-specific diversification of pathogen-inducible tryptophan and indole glucosinolate metabolism in Arabidopsis thaliana relatives

2011 ◽  
Vol 192 (3) ◽  
pp. 713-726 ◽  
Author(s):  
Paweł Bednarek ◽  
Mariola Piślewska-Bednarek ◽  
Emiel Ver Loren van Themaat ◽  
Ravi Kumar Maddula ◽  
Aleš Svatoš ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Indole Glucosinolate ◽  
Glucosinolate Metabolism

scholarly journals Multiple indole glucosinolates and myrosinases defend Arabidopsis against Tetranychus urticae herbivory

2021 ◽  
Author(s):  
Emilie Widemann ◽  
Kristie Bruinsma ◽  
Brendan Walshe-Roussel ◽  
Repon Kumer Saha ◽  
David Letwin ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factors ◽  
Tetranychus Urticae ◽  
Metabolic Pathways ◽  
Spider Mite ◽  
Generalist Herbivore ◽  
Defense Compounds ◽  
Indole Glucosinolates ◽  
Indole Glucosinolate ◽  
Two Spotted Spider Mite

ABSTRACTArabidopsis defenses against herbivores are regulated by the jasmonate hormonal signaling pathway, which leads to the production of a plethora of defense compounds, including tryptophan-derived metabolites produced through CYP79B2/CYP79B3. Jasmonate signaling and CYP79B2/CYP79B3 limit Arabidopsis infestation by the generalist herbivore two-spotted spider mite, Tetranychus urticae. However, the phytochemicals responsible for Arabidopsis protection against T. urticae are unknown. Here, using Arabidopsis mutants that disrupt metabolic pathways downstream of CYP79B2/CYP79B3, and synthetic indole glucosinolates, we identified phytochemicals involved in the defense against T. urticae. We show that Trp-derived metabolites depending on CYP71A12 and CYP71A13 are not affecting mite herbivory. Instead, the supplementation of cyp79b2 cyp79b3 mutant leaves with the 3-indolylmethyl glucosinolate and its derived metabolites demonstrated that the indole glucosinolate pathway is sufficient to assure CYP79B2/CYP79B3-mediated defenses against T. urticae. We demonstrate that three indole glucosinolates can limit T. urticae herbivory, but that they have to be processed by the myrosinases to hinder T. urticae oviposition. Finally, the supplementation of the mutant myc2 myc3 myc4 with indole glucosinolates indicated that the transcription factors MYC2/MYC3/MYC4 induce additional indole glucosinolate-independent defenses that control T. urticae herbivory. Together, these results reveal the complexity of Arabidopsis defenses against T. urticae that rely on multiple indole glucosinolates, specific myrosinases, and additional MYC2/MYC3/MYC4-dependent defenses.One sentence summaryThree indole glucosinolates and the myrosinases TGG1/TGG2 help protect Arabidopsis thaliana against the herbivory of the two-spotted spider mite Tetranychus urticae.

scholarly journals CRISPR-Cas9-mediated editing of myb28 impairs glucoraphanin accumulation of Brassica oleracea in the field

2020 ◽  
Author(s):  
Mikhaela Neequaye ◽  
Sophia Stavnstrup ◽  
Tom Lawrenson ◽  
Penny Hundleby ◽  
Perla Troncoso-Rey ◽  
...  
Keyword(s):  
Gene Editing ◽  
European Court Of Justice ◽  
Knockout Mutant ◽  
Glucosinolate Biosynthesis ◽  
Court Of Justice ◽  
European Court ◽  
Indole Glucosinolate ◽  
Cysteine Sulfoxide ◽  
Aliphatic Glucosinolate

SummaryWe sought to quantify the role of MYB28 in the regulation of aliphatic glucosinolate biosynthesis and associated sulphur metabolism in field-grown B. oleracea with the use of CRISPR-Cas9-mediated gene editing technology. We describe the first characterised myb28 knockout mutant in B. oleracea, and the first UK field trial of CRISPR-Cas9-mediated gene edited plants under the European Court of Justice interpretation of the 2001/18 EU GMO directive. We report that knocking-out myb28 results in downregulation of aliphatic glucosinolate biosynthesis genes and reduction in accumulation of the methionine-derived glucosinolate, glucoraphanin, in leaves and florets of field-grown myb28 mutant broccoli plants. There were no significant changes to the accumulation of sulphate, S-methyl cysteine sulfoxide and indole glucosinolate in leaf and floret tissues.

scholarly journals β-Lactam Antibiotics Modify Root Architecture and Indole Glucosinolate Metabolism in Arabidopsis thaliana

2018 ◽  
Vol 59 (10) ◽  
pp. 2086-2098 ◽  
Author(s):  
Marco E Gudi�o ◽  
Noel Blanco-Touri��n ◽  
Vicent Arbona ◽  
Aurelio G�mez-Cadenas ◽  
Miguel A Bl�zquez ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Root Architecture ◽  
Indole Glucosinolate ◽  
Glucosinolate Metabolism
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