Indole glucosinolate and auxin biosynthesis in Arabidopsis thaliana (L.) Heynh. glucosinolate mutants and the development of clubroot disease

Planta ◽  
1999 ◽  
Vol 208 (3) ◽  
pp. 409-419 ◽  
Author(s):  
Jutta Ludwig-Müller ◽  
Kerstin Pieper ◽  
Manfred Ruppel ◽  
Jerry D. Cohen ◽  
Ephraim Epstein ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Auxin Biosynthesis ◽  
Clubroot Disease ◽  
Indole Glucosinolate

scholarly journals Conservation and clade-specific diversification of pathogen-inducible tryptophan and indole glucosinolate metabolism in Arabidopsis thaliana relatives

2011 ◽  
Vol 192 (3) ◽  
pp. 713-726 ◽  
Author(s):  
Paweł Bednarek ◽  
Mariola Piślewska-Bednarek ◽  
Emiel Ver Loren van Themaat ◽  
Ravi Kumar Maddula ◽  
Aleš Svatoš ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Indole Glucosinolate ◽  
Glucosinolate Metabolism

scholarly journals Multiple indole glucosinolates and myrosinases defend Arabidopsis against Tetranychus urticae herbivory

2021 ◽  
Author(s):  
Emilie Widemann ◽  
Kristie Bruinsma ◽  
Brendan Walshe-Roussel ◽  
Repon Kumer Saha ◽  
David Letwin ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factors ◽  
Tetranychus Urticae ◽  
Metabolic Pathways ◽  
Spider Mite ◽  
Generalist Herbivore ◽  
Defense Compounds ◽  
Indole Glucosinolates ◽  
Indole Glucosinolate ◽  
Two Spotted Spider Mite

ABSTRACTArabidopsis defenses against herbivores are regulated by the jasmonate hormonal signaling pathway, which leads to the production of a plethora of defense compounds, including tryptophan-derived metabolites produced through CYP79B2/CYP79B3. Jasmonate signaling and CYP79B2/CYP79B3 limit Arabidopsis infestation by the generalist herbivore two-spotted spider mite, Tetranychus urticae. However, the phytochemicals responsible for Arabidopsis protection against T. urticae are unknown. Here, using Arabidopsis mutants that disrupt metabolic pathways downstream of CYP79B2/CYP79B3, and synthetic indole glucosinolates, we identified phytochemicals involved in the defense against T. urticae. We show that Trp-derived metabolites depending on CYP71A12 and CYP71A13 are not affecting mite herbivory. Instead, the supplementation of cyp79b2 cyp79b3 mutant leaves with the 3-indolylmethyl glucosinolate and its derived metabolites demonstrated that the indole glucosinolate pathway is sufficient to assure CYP79B2/CYP79B3-mediated defenses against T. urticae. We demonstrate that three indole glucosinolates can limit T. urticae herbivory, but that they have to be processed by the myrosinases to hinder T. urticae oviposition. Finally, the supplementation of the mutant myc2 myc3 myc4 with indole glucosinolates indicated that the transcription factors MYC2/MYC3/MYC4 induce additional indole glucosinolate-independent defenses that control T. urticae herbivory. Together, these results reveal the complexity of Arabidopsis defenses against T. urticae that rely on multiple indole glucosinolates, specific myrosinases, and additional MYC2/MYC3/MYC4-dependent defenses.One sentence summaryThree indole glucosinolates and the myrosinases TGG1/TGG2 help protect Arabidopsis thaliana against the herbivory of the two-spotted spider mite Tetranychus urticae.

scholarly journals β-Lactam Antibiotics Modify Root Architecture and Indole Glucosinolate Metabolism in Arabidopsis thaliana

2018 ◽  
Vol 59 (10) ◽  
pp. 2086-2098 ◽  
Author(s):  
Marco E Gudi�o ◽  
Noel Blanco-Touri��n ◽  
Vicent Arbona ◽  
Aurelio G�mez-Cadenas ◽  
Miguel A Bl�zquez ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Root Architecture ◽  
Indole Glucosinolate ◽  
Glucosinolate Metabolism

scholarly journals Indole Glucosinolate Biosynthesis Limits Phenylpropanoid Accumulation in Arabidopsis thaliana

2015 ◽  
Vol 27 (5) ◽  
pp. 1529-1546 ◽  
Author(s):  
Jeong Im Kim ◽  
Whitney L. Dolan ◽  
Nickolas A. Anderson ◽  
Clint Chapple
Keyword(s):  
Arabidopsis Thaliana ◽  
Glucosinolate Biosynthesis ◽  
Indole Glucosinolate

Plant nitrilase: a new job for an old enzyme

2019 ◽  
Vol 476 (7) ◽  
pp. 1105-1107
Author(s):  
Joseph M. Jez
Keyword(s):  
Arabidopsis Thaliana ◽  
Recent Work ◽  
Carboxylic Acids ◽  
Auxin Biosynthesis ◽  
Biological Functions ◽  
Repair Enzyme ◽  
Biochemical Journal ◽  
Metabolite Repair

Abstract Nitrilases are versatile enzymes that hydrolyze nitriles to carboxylic acids and ammonia, but many members of this family lack defined biological functions. In plants, nitrilases have been associated with detoxification of cyanide-containing compounds and auxin biosynthesis; however, recent work suggests that the chemical versatility of these proteins contributes to metabolite repair. In this issue of the Biochemical Journal, Niehaus et al. demonstrate that the Nit1 nitrilase from Arabidopsis thaliana functions as a metabolite repair enzyme that removes deaminated glutathione from the cytoplasm and plastids.

scholarly journals Regulation of Pathogen-Triggered Tryptophan Metabolism in Arabidopsis thaliana by MYB Transcription Factors and Indole Glucosinolate Conversion Products

2016 ◽  
Vol 9 (5) ◽  
pp. 682-695 ◽  
Author(s):  
Henning Frerigmann ◽  
Mariola Piślewska-Bednarek ◽  
Andrea Sánchez-Vallet ◽  
Antonio Molina ◽  
Erich Glawischnig ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factors ◽  
Tryptophan Metabolism ◽  
Myb Transcription Factors ◽  
Indole Glucosinolate

scholarly journals The Arabidopsis thaliana STYLISH1 Protein Acts as a Transcriptional Activator Regulating Auxin Biosynthesis

2010 ◽  
Vol 22 (2) ◽  
pp. 349-363 ◽  
Author(s):  
D. Magnus Eklund ◽  
Veronika Ståldal ◽  
Isabel Valsecchi ◽  
Izabela Cierlik ◽  
Caitriona Eriksson ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcriptional Activator ◽  
Auxin Biosynthesis

scholarly journals A Novel Target (Oxidation Resistant 2) in Arabidopsis thaliana to Reduce Clubroot Disease Symptoms via the Salicylic Acid Pathway without Growth Penalties

Horticulturae ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 9
Author(s):  
Regina Mencia ◽  
Elina Welchen ◽  
Susann Auer ◽  
Jutta Ludwig-Müller
Keyword(s):  
Arabidopsis Thaliana ◽  
Salicylic Acid ◽  
Plant Growth ◽  
Plasmodiophora Brassicae ◽  
Gene Encoding ◽  
Clubroot Disease ◽  
Disease Symptoms ◽  
Oxidation Resistant ◽  
Acid Pathway ◽  
Novel Target

The clubroot disease (Plasmodiophora brassicae) is one of the most damaging diseases worldwide among brassica crops. Its control often relies on resistant cultivars, since the manipulation of the disease hormones, such as salicylic acid (SA) alters plant growth negatively. Alternatively, the SA pathway can be increased by the addition of beneficial microorganisms for biocontrol. However, this potential has not been exhaustively used. In this study, a recently characterized protein Oxidation Resistant 2 (OXR2) from Arabidopsis thaliana is shown to increase the constitutive pathway of SA defense without decreasing plant growth. Plants overexpressing AtOXR2 (OXR2-OE) show strongly reduced clubroot symptoms with improved plant growth performance, in comparison to wild type plants during the course of infection. Consequently, oxr2 mutants are more susceptible to clubroot disease. P. brassicae itself was reduced in these galls as determined by quantitative real-time PCR. Furthermore, we provide evidence for the transcriptional downregulation of the gene encoding a SA-methyltransferase from the pathogen in OXR2-OE plants that could contribute to the phenotype.

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