scholarly journals Methyl Transfer in Glucosinolate Biosynthesis Mediated by Indole Glucosinolate O-Methyltransferase 5

2016 ◽  
Vol 172 (4) ◽  
pp. 2190-2203 ◽  
Author(s):  
Marina Pfalz ◽  
Maisara Mukhaimar ◽  
François Perreau ◽  
Jayne Kirk ◽  
Cecilie Ida Cetti Hansen ◽  
...  
Keyword(s):  
Glucosinolate Biosynthesis ◽  
Methyl Transfer ◽  
Indole Glucosinolate

scholarly journals CRISPR-Cas9-mediated editing of myb28 impairs glucoraphanin accumulation of Brassica oleracea in the field

2020 ◽  
Author(s):  
Mikhaela Neequaye ◽  
Sophia Stavnstrup ◽  
Tom Lawrenson ◽  
Penny Hundleby ◽  
Perla Troncoso-Rey ◽  
...  
Keyword(s):  
Gene Editing ◽  
European Court Of Justice ◽  
Knockout Mutant ◽  
Glucosinolate Biosynthesis ◽  
Court Of Justice ◽  
European Court ◽  
Indole Glucosinolate ◽  
Cysteine Sulfoxide ◽  
Aliphatic Glucosinolate

SummaryWe sought to quantify the role of MYB28 in the regulation of aliphatic glucosinolate biosynthesis and associated sulphur metabolism in field-grown B. oleracea with the use of CRISPR-Cas9-mediated gene editing technology. We describe the first characterised myb28 knockout mutant in B. oleracea, and the first UK field trial of CRISPR-Cas9-mediated gene edited plants under the European Court of Justice interpretation of the 2001/18 EU GMO directive. We report that knocking-out myb28 results in downregulation of aliphatic glucosinolate biosynthesis genes and reduction in accumulation of the methionine-derived glucosinolate, glucoraphanin, in leaves and florets of field-grown myb28 mutant broccoli plants. There were no significant changes to the accumulation of sulphate, S-methyl cysteine sulfoxide and indole glucosinolate in leaf and floret tissues.

scholarly journals Indole Glucosinolate Biosynthesis Limits Phenylpropanoid Accumulation in Arabidopsis thaliana

2015 ◽  
Vol 27 (5) ◽  
pp. 1529-1546 ◽  
Author(s):  
Jeong Im Kim ◽  
Whitney L. Dolan ◽  
Nickolas A. Anderson ◽  
Clint Chapple
Keyword(s):  
Arabidopsis Thaliana ◽  
Glucosinolate Biosynthesis ◽  
Indole Glucosinolate

CYP83B1, a Cytochrome P450 at the Metabolic Branch Point in Auxin and Indole Glucosinolate Biosynthesis in Arabidopsis

2001 ◽  
Vol 13 (1) ◽  
pp. 101 ◽  
Author(s):  
Soren Bak ◽  
Frans E. Tax ◽  
Kenneth A. Feldmann ◽  
David W. Galbraith ◽  
Rene Feyereisen
Keyword(s):  
Cytochrome P450 ◽  
Branch Point ◽  
Glucosinolate Biosynthesis ◽  
Indole Glucosinolate

scholarly journals CYP83B1, a Cytochrome P450 at the Metabolic Branch Point in Auxin and Indole Glucosinolate Biosynthesis in Arabidopsis

2001 ◽  
Vol 13 (1) ◽  
pp. 101-111 ◽  
Author(s):  
Søren Bak ◽  
Frans E. Tax ◽  
Kenneth A. Feldmann ◽  
David W. Galbraith ◽  
René Feyereisen
Keyword(s):  
Cytochrome P450 ◽  
Branch Point ◽  
Glucosinolate Biosynthesis ◽  
Indole Glucosinolate

scholarly journals Studies on Methyl Transfer from 5,6-Dimethylbenzimidazolylcobamide Methyl (Methyl-B12) to Homocysteine

1964 ◽  
Vol 239 (6) ◽  
pp. 1942-1946 ◽  
Author(s):  
Herbert Weissbach ◽  
Betty G. Redfield ◽  
Herbert Dickerman
Keyword(s):  
Methyl Methyl ◽  
Methyl Transfer

scholarly journals Overall Retention of Methyl Stereochemistry during B12-Dependent Radical SAM Methyl Transfer in Fosfomycin Biosynthesis

Biochemistry ◽  
2021 ◽  
Author(s):  
Martin I. McLaughlin ◽  
Katharina Pallitsch ◽  
Gabriele Wallner ◽  
Wilfred A. van der Donk ◽  
Friedrich Hammerschmidt
Keyword(s):  
Radical Sam ◽  
Methyl Transfer
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