scholarly journals Effect of Gibberellic Acid on Crown Gall Tumor Induction in Aging Primary Pinto Bean Leaves

1975 ◽  
Vol 55 (6) ◽  
pp. 1016-1017 ◽  
Author(s):  
Vinod K. Anand ◽  
Chris Bauer ◽  
Gary T. Heberlein
Keyword(s):  
Gibberellic Acid ◽  
Crown Gall ◽  
Tumor Induction ◽  
Crown Gall Tumor ◽  
Pinto Bean ◽  
Bean Leaves

Timing of events in crown-gall tumor development on pinto bean leaves

1965 ◽  
Vol 12 (2) ◽  
pp. 309-327 ◽  
Author(s):  
James A. Lippincott ◽  
Barbara B. Lippincott
Keyword(s):  
Crown Gall ◽  
Tumor Development ◽  
Crown Gall Tumor ◽  
Pinto Bean ◽  
Bean Leaves

Polymyxin resistance in Agrobacterium tumefaciens and its effect on crown gall tumor induction

1979 ◽  
Vol 25 (2) ◽  
pp. 185-191 ◽  
Author(s):  
C. H. Liao ◽  
G. T. Heberlein
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Host Plants ◽  
Polymyxin B ◽  
Defined Medium ◽  
Tumor Initiation ◽  
Tumor Induction ◽  
Crown Gall Tumor ◽  
Pinto Bean ◽  
Cell Envelopes

Polymyxin-resistant (PBLr) mutants of Agrobacterium tumefaciens A6, B6, and B6M were isolated from polymyxin-sensitive (PBLs) parent strains in a defined medium containing 600 μg of polymyxin B sulfate per millilitre. The weight and number of tumors induced by PBLr mutants on a variety of host plants such as carrot, potato, and pinto bean were 45–75% less than those induced by PBLs wild types. The crude cell envelopes (CCE) prepared from both PBLs and PBLr bacteria were inhibitory for tumor initiation when they were applied before or during the inoculation of viable tumorigenic bacteria, but not when they were applied 30 min after the inoculation of infectious bacteria. The potency to inhibit the tumor initiation by the CCE prepared from PBLs cells was approximately 50% higher than that by the equal amount of the CCE prepared from PBLr cells. The concentration of CCE preparations required to reduce tumor induction 50% in carrot and pinto bean was determined to be 2.6 mg/mL and 4.0–6.2 mg/mL for the CCE derived from PBLs and PBLr cells, respectively. These data suggest that the envelope structure or composition of PBLs and PBLr cells is distinct, and that the acquisition of resistance to polymyxin by agrobacteria modifies envelope structure or components which are essential for tumor initiation.

Effect of rifampin application time on crown-gall tumor induction by Agrobacterium tumefaciens

1975 ◽  
Vol 53 (22) ◽  
pp. 2581-2588 ◽  
Author(s):  
V. K. Anand ◽  
G. T. Heberlein
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Lethal Effect ◽  
Inhibitory Effect ◽  
Bacterial Inoculation ◽  
Plant Host ◽  
Tumor Induction ◽  
Time Period ◽  
Pinto Bean ◽  
Bean Leaves

Rifampin was applied to Kalanchöe leaves, primary pinto-bean leaves, and carrot root discs at various times after inoculation with Agrobacterium tumefaciens. Inhibition of tumor induction was substantial and maximal when rifampin (200 μg/ml) was applied to the wound sites immediately after bacterial inoculation. The time period after inoculation during which rifampin remained inhibitory varied with the plant host used. Inoculated Kalanchöe leaves remained sensitive to rifampin for 36 h while pinto-bean leaves and carrot discs remained sensitive for 20 and 4 h, respectively. Rifampin failed to inhibit tumor induction when applied to plant wounds inoculated with rifampin-resistant mutants of A. tumefaciens. Tumor weight was typically enhanced by rifampin in the Kalanchöe and carrot assays when applied after the period in which it was inhibitory. The viability of A. tumefaciens in vitro and while on the plant was substantially reduced by rifampin. Nevertheless, the lethal effect of the drug on A. tumefaciens was insufficient to fully account for its inhibitory effect on tumor induction.

The role of nucleic acids in crown-gall tumor induction

1958 ◽  
Vol 14 (11) ◽  
pp. 409-410 ◽  
Author(s):  
P. Manigault ◽  
Ch. Stoll
Keyword(s):  
Nucleic Acids ◽  
Crown Gall ◽  
Tumor Induction ◽  
Crown Gall Tumor

scholarly journals Cumulative Cytotoxicity Assay of the Aqueous and Ethanolic Extracts of the Selected Medicinal Plants using Crown Gall Tumor Disc Bioassay

2020 ◽  
Author(s):  
Sandeep Waghulde ◽  
Tushar Baviskar ◽  
Praful Patil ◽  
Shweta Singh ◽  
Nilesh Gorde ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Crown Gall ◽  
Cytotoxicity Assay ◽  
Crown Gall Tumor ◽  
Ethanolic Extracts
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