The role of nucleic acids in crown-gall tumor induction

1958 ◽  
Vol 14 (11) ◽  
pp. 409-410 ◽  
Author(s):  
P. Manigault ◽  
Ch. Stoll
Keyword(s):  
Nucleic Acids ◽  
Crown Gall ◽  
Tumor Induction ◽  
Crown Gall Tumor

On the Role of Omega Bacteriophage in Formation of Crown-Gall Tumor Cells

1957 ◽  
Vol 91 (860) ◽  
pp. 330-331 ◽  
Author(s):  
Richard M. Klein ◽  
Robert E. Beardsley
Keyword(s):  
Tumor Cells ◽  
Crown Gall ◽  
Crown Gall Tumor

scholarly journals Effect of Gibberellic Acid on Crown Gall Tumor Induction in Aging Primary Pinto Bean Leaves

1975 ◽  
Vol 55 (6) ◽  
pp. 1016-1017 ◽  
Author(s):  
Vinod K. Anand ◽  
Chris Bauer ◽  
Gary T. Heberlein
Keyword(s):  
Gibberellic Acid ◽  
Crown Gall ◽  
Tumor Induction ◽  
Crown Gall Tumor ◽  
Pinto Bean ◽  
Bean Leaves

Polymyxin resistance in Agrobacterium tumefaciens and its effect on crown gall tumor induction

1979 ◽  
Vol 25 (2) ◽  
pp. 185-191 ◽  
Author(s):  
C. H. Liao ◽  
G. T. Heberlein
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Host Plants ◽  
Polymyxin B ◽  
Defined Medium ◽  
Tumor Initiation ◽  
Tumor Induction ◽  
Crown Gall Tumor ◽  
Pinto Bean ◽  
Cell Envelopes

Polymyxin-resistant (PBLr) mutants of Agrobacterium tumefaciens A6, B6, and B6M were isolated from polymyxin-sensitive (PBLs) parent strains in a defined medium containing 600 μg of polymyxin B sulfate per millilitre. The weight and number of tumors induced by PBLr mutants on a variety of host plants such as carrot, potato, and pinto bean were 45–75% less than those induced by PBLs wild types. The crude cell envelopes (CCE) prepared from both PBLs and PBLr bacteria were inhibitory for tumor initiation when they were applied before or during the inoculation of viable tumorigenic bacteria, but not when they were applied 30 min after the inoculation of infectious bacteria. The potency to inhibit the tumor initiation by the CCE prepared from PBLs cells was approximately 50% higher than that by the equal amount of the CCE prepared from PBLr cells. The concentration of CCE preparations required to reduce tumor induction 50% in carrot and pinto bean was determined to be 2.6 mg/mL and 4.0–6.2 mg/mL for the CCE derived from PBLs and PBLr cells, respectively. These data suggest that the envelope structure or composition of PBLs and PBLr cells is distinct, and that the acquisition of resistance to polymyxin by agrobacteria modifies envelope structure or components which are essential for tumor initiation.

scholarly journals Octopine and nopaline metabolism in Agrobacterium tumefaciens and crown gall tumor cells: role of plasmid genes.

1977 ◽  
Vol 129 (1) ◽  
pp. 101-107 ◽  
Author(s):  
A L Montoya ◽  
M D Chilton ◽  
M P Gordon ◽  
D Sciaky ◽  
E W Nester
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Tumor Cells ◽  
Crown Gall ◽  
Crown Gall Tumor ◽  
Plasmid Genes

scholarly journals ROLE OF SUSPENDED PARTICULATE MATTER IN ANGIOGENESIS EMPLOYING CROWN GALL TUMOR ASSAY

2016 ◽  
Vol 9 (1) ◽  
pp. 34 ◽  
Author(s):  
Simerpreet Kaur ◽  
Jatinder K. Katnoria
Keyword(s):  
Particulate Matter ◽  
Suspended Particulate Matter ◽  
Crown Gall ◽  
High Volume ◽  
Screening Assay ◽  
Traffic Emission ◽  
Crown Gall Tumor ◽  
Suspended Particulate ◽  
Mechanical Shaker

<p><strong>Objective: </strong>Aim of the study was to screen the suspended particulate matter for their role in enhancing angiogenesis employing crown gall tumor assay.</p><p><strong>Methods: </strong>Particulate matter (PM<sub>10</sub>) samples were collected, on glass fiber filter paper at the rate of 1.12–1.14 m<sup>3/</sup>min for 8 hr using high volume sampler, from six different sites of Amritsar city. Aqueous extracts of suspended particulate matter were prepared using a mechanical shaker for 24 hour and the filtrate was centrifuged and lyophilized.</p><p><strong>Results: </strong>Maximum (34.67±1.764) tumors were induced by samples collected from Crystal chowk whereas tumor inducing ratio (TIR) was found to be comparably high for two sites <em>viz.,</em> Crystal chowk and Bhandari bridge.</p><p><strong>Conclusion: </strong>High tumor inducing ratio at the above-mentioned sites was correlated to high traffic emission from automobile exhaust. Crown gall tumor assay has been proved to be rapid, economical and reliable screening assay for angiogenesis agent.</p>

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