scholarly journals Indoleacetic Acid Biosynthesis in Avena Coleoptile Tips and Excised Bean Shoots

1971 ◽  
Vol 48 (5) ◽  
pp. 603-606 ◽  
Author(s):  
Robert C. Black ◽  
Robert H. Hamilton
Keyword(s):  
Indoleacetic Acid ◽  
Acid Biosynthesis ◽  
Avena Coleoptile ◽  
Indoleacetic Acid Biosynthesis

scholarly journals Proteome and Transcriptome Reveal Involvement of Heat Shock Proteins and Indoleacetic Acid Metabolism Process in Lentinula Edodes Thermotolerance

2018 ◽  
Vol 50 (5) ◽  
pp. 1617-1637 ◽  
Author(s):  
Gang-Zheng Wang ◽  
Chao-Jun Ma ◽  
Yi Luo ◽  
Sha-Sha Zhou ◽  
Yan Zhou ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Indoleacetic Acid ◽  
Lentinula Edodes ◽  
Acid Biosynthesis ◽  
Over Expression ◽  
Indoleacetic Acid Biosynthesis ◽  
Shock Proteins ◽  
Exogenous Iaa

Background/Aims: Heat stress could cause huge losses for Lentinula edodes in China and other Asian cultivation areas. Yet our understanding of mechanism how to defend to heat stress is incomplete. Methods: Using heat-tolerant and heat-sensitive strains of L. edodes, we reported a combined proteome and transcriptome analysis of L. edodes response to 40 °C heat stress for 24 h. Meanwhile, the effect of LeDnaJ on the thermotolerance and IAA (indoleacetic acid) biosynthesis in L. edodes was analyzed via the over-expression method. Results: The proteome results revealed that HSPs (heat shock proteins) such as Hsp40 (DnaJ), Hsp70, Hsp90 and key enzymes involved in tryptophan and IAA metabolism process LeTrpE, LeTrpD, LeTam-1, LeYUCCA were more highly expressed in S606 than in YS3357, demonstrating that HSPs and tryptophan as well as IAA metabolism pathway should play an important role in thermotolerance. Over-expression of LeDnaJ gene in S606 strains showed better tolerance to heat stress. It was also documented that intracellular IAA accumulation of S606 (8-fold up) was more than YS3357 (2-fold up), and exogenous IAA enhanced L. edodes tolerance to heat stress. Conclusion: Our data support the interest of LeTrpE, LeDnaJ, tryptophan and IAA could play a pivotal role in enhancing organism thermotolerance.

scholarly journals Detection of indoleacetic acid biosynthesis in some species of Taphrina causing hyperplastic diseases in plants.

1990 ◽  
Vol 56 (4) ◽  
pp. 532-540 ◽  
Author(s):  
Tetsuji YAMADA ◽  
Hiroshi TSUKAMOTO ◽  
Tomonori SHIRAISHI ◽  
Tetsuya NOMURA ◽  
Hachiro OKU
Keyword(s):  
Indoleacetic Acid ◽  
Acid Biosynthesis ◽  
Indoleacetic Acid Biosynthesis

THE PHYSIOLOGY OF HOST–PARASITE RELATIONS: V. A PRELIMINARY EXAMINATION OF THE LEVEL OF FREE ENDOGENOUS INDOLEACETIC ACID IN RUSTED AND MILDEWED CEREAL LEAVES AND THEIR ABILITY TO DECARBOXYLATE EXOGENOUSLY SUPPLIED RADIOACTIVE INDOLEACETIC ACID

1958 ◽  
Vol 36 (1) ◽  
pp. 1-16 ◽  
Author(s):  
Michael Shaw ◽  
A. R. Hawkins
Keyword(s):  
Oxidase Activity ◽  
Indoleacetic Acid ◽  
Sharp Decrease ◽  
Inhibiting Activity ◽  
Growth Substances ◽  
Fresh Weight ◽  
Avena Coleoptile ◽  
Preliminary Examination ◽  
Growth Promoting ◽  
Host Parasite

The growth substances were extracted with cold alcohol from the first leaves of uninfected, rusted (wheat), and mildewed (barley) cereal seedlings. The acid ether fractions were chromatographed on paper and the chromatograms were cut into sections which were assayed for growth promoting or inhibiting activity in the Avena coleoptile straight growth test. The estimated, free, endogenous indoleacetic acid content of uninfected leaves ranged from 0.5 to 3.2 μg. per kilogram fresh weight. In the early stages of infection this decreased, but increased again to from 5 to about 10 μg. per kilogram fresh weight by the 10th day after the inoculation of susceptible hosts. Indoleacetic acid was not detected in ungerminated uredospores of stem rust (race 15B), but two other growth promoting substances appeared to be present.Leaf disks were incubated with radioactive indoleacetic acid (as —C14OOK) and the radioactivity released as C14O2 was measured. The ability of the tissue to decarboxylate the indoleacetate (‘oxidase’ activity) increased sharply, sometimes to as much as 1000%, in the first 3 days after inoculation. With susceptible hosts, this increase was followed by an almost equally sharp decrease to less than 50% of the values for uninfected tissue. With infected, resistant tissue, the secondary decrease in ‘oxidase’ activity was delayed and less pronounced.The results are discussed and a working hypothesis suggested with respect to the relation between susceptibility or resistance and the auxin balance.

Protein synthesis and leucine uptake during fusicoccin-stimulated growth of oat coleoptile tissue

1980 ◽  
Vol 58 (22) ◽  
pp. 2356-2359 ◽  
Author(s):  
Ahmed C. Doo ◽  
Alan W. Bown
Keyword(s):  
Protein Synthesis ◽  
Indoleacetic Acid ◽  
Soluble Fraction ◽  
Leucine Incorporation ◽  
Avena Coleoptile ◽  
Total Uptake ◽  
Leucine Uptake ◽  
Significant Difference ◽  
Soluble Fractions

Avena coleoptile sections were incubated with or without cycloheximide (CHI) in solutions containing indoleacetic acid (IAA), fusicoccin (FC), or IAA and FC. Resulting growth, incorporation of [3H]leucine into protein, and uptake of [3H]leucine into an ethanol-soluble fraction were determined. FC-stimulated growth was greater and less sensitive to CHI treatment than IAA dependant growth which was virtually eliminated by CHI. IAA alone had little or no influence on [3H]leucine utilization, whereas FC treatment stimulated [3H]leucine incorporation into protein by 36% and uptake into the ethanol-soluble fraction by 75%. CHI inhibited the incorporation of [3H]leucine label into protein such that no significant difference was observed in radioactivity in protein from control, FC-, or IAA-treated tissue. However, CHI did not inhibit the FC-stimulated uptake of [3H]leucine into the ethanol soluble fraction. Total uptake of [3H]leucine obtained from the sum of radioactivities in the protein and ethanol-soluble fractions was stimulated by FC approximately 54% in the absence of CHI and 92% in the presence of CHI. IAA in combination with FC stimulated a further increase in leucine uptake. It is proposed that IAA and FC stimulate growth through processes which differ in their dependency on protein synthesis, and that FC-stimulated incorporation of label into protein results from FC-stimulated leucine uptake, not FC-stimulated protein synthesis.

scholarly journals Responses of Avena Coleoptile Sections to High Concentrations of Auxin

1957 ◽  
Vol 10 (2) ◽  
pp. 147 ◽  
Author(s):  
NG Marinos
Keyword(s):  
Respiratory Rate ◽  
Initial Phase ◽  
Indoleacetic Acid ◽  
Elongation Rate ◽  
Solid Matter ◽  
Avena Coleoptile ◽  
High Concentrations

(i) The initial elongation rate of excised coleoptile sections is shown to increase with increasing concentration of indoleacetic acid (IAA). In supra-optimal concentrations (1 X 1O-3M) this initial phase is followed by shrinkage of the sections accompanied by loss of water and solid matter; the latter is quantitatively recoverable in the solution. The respiratory rate drops rapidly while the sections elongate in 1 X 10-3M IAA.

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