scholarly journals Pathway-Level Acceleration of Glycogen Catabolism by a Response Regulator in the Cyanobacterium Synechocystis Species PCC 6803

2014 ◽  
Vol 164 (4) ◽  
pp. 1831-1841 ◽  
Author(s):  
Takashi Osanai ◽  
Akira Oikawa ◽  
Keiji Numata ◽  
Ayuko Kuwahara ◽  
Hiroko Iijima ◽  
...  
Keyword(s):  
Response Regulator ◽  
Pathway Level ◽  
Glycogen Catabolism ◽  
Pcc 6803

scholarly journals The nitrogen-regulated response regulator NrrA is a conserved regulator of glycogen catabolism in β-cyanobacteria

Microbiology ◽  
2017 ◽  
Vol 163 (11) ◽  
pp. 1711-1719 ◽  
Author(s):  
Shigeki Ehira ◽  
Yuka Shimmori ◽  
Satoru Watanabe ◽  
Hiroaki Kato ◽  
Hirofumi Yoshikawa ◽  
...  
Keyword(s):  
Response Regulator ◽  
Glycogen Catabolism

scholarly journals The Response Regulator RpaB Binds to the Upstream Element of Photosystem I Genes To Work for Positive Regulation under Low-Light Conditions in Synechocystis sp. Strain PCC 6803

2008 ◽  
Vol 191 (5) ◽  
pp. 1581-1586 ◽  
Author(s):  
Yurie Seino ◽  
Tomoko Takahashi ◽  
Yukako Hihara
Keyword(s):  
Photosystem I ◽  
Promoter Activity ◽  
Response Regulator ◽  
Core Promoter ◽  
High Light ◽  
Light Conditions ◽  
Low Light ◽  
Positive Regulation ◽  
Upstream Element ◽  
Pcc 6803

ABSTRACT The coordinated high-light response of genes encoding subunits of photosystem I (PSI) is achieved by the AT-rich region located just upstream of the core promoter in Synechocystis sp. strain PCC 6803. The upstream element enhances the basal promoter activity under low-light conditions, whereas this positive regulation is lost immediately after the shift to high-light conditions. In this study, we focused on a high-light regulatory 1 (HLR1) sequence included in the upstream element of every PSI gene examined. A gel mobility shift assay revealed that a response regulator RpaB binds to the HLR1 sequence in PSI promoters. Base substitution in the HLR1 sequence or decrease in copy number of the rpaB gene resulted in decrease in the promoter activity of PSI genes under low-light conditions. These observations suggest that RpaB acts as a transcriptional activator for PSI genes. It is likely that RpaB binds to the HLR1 sequence under low-light conditions and works for positive regulation of PSI genes and for negative regulation of high-light-inducible genes depending on the location of the HLR1 sequence within target promoters.

Elucidating butanol tolerance mediated by a response regulator Sll0039 in Synechocystis sp. PCC 6803 using a metabolomic approach

2015 ◽  
Vol 99 (4) ◽  
pp. 1845-1857 ◽  
Author(s):  
Xiangfeng Niu ◽  
Ye Zhu ◽  
Guangsheng Pei ◽  
Lina Wu ◽  
Lei Chen ◽  
...  
Keyword(s):  
Response Regulator ◽  
Butanol Tolerance ◽  
Metabolomic Approach ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Slr0967 and Sll0939 induced by the SphR response regulator in Synechocystis sp. PCC 6803 are essential for growth under acid stress conditions

2012 ◽  
Vol 1817 (8) ◽  
pp. 1270-1276 ◽  
Author(s):  
Junji Uchiyama ◽  
Ryosuke Asakura ◽  
Mayuko Kimura ◽  
Atsushi Moriyama ◽  
Hiroko Tahara ◽  
...  
Keyword(s):  
Response Regulator ◽  
Acid Stress ◽  
Stress Conditions ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Characterization of an Alcohol Dehydrogenase from the Cyanobacterium Synechocystis sp. Strain PCC 6803 That Responds to Environmental Stress Conditions via the Hik34-Rre1 Two-Component System

2009 ◽  
Vol 191 (13) ◽  
pp. 4383-4391 ◽  
Author(s):  
Rebeca Vidal ◽  
Luis López-Maury ◽  
Miguel G. Guerrero ◽  
Francisco J. Florencio
Keyword(s):  
Alcohol Dehydrogenase ◽  
Interaction Analysis ◽  
Response Regulator ◽  
Stress Conditions ◽  
Primary Alcohols ◽  
Two Component System ◽  
Component System ◽  
Two Component ◽  
Pcc 6803

ABSTRACT The slr1192 (adhA) gene from Synechocystis sp. strain PCC 6803 encodes a member of the medium-chain alcohol dehydrogenase/reductase family. The gene product AdhA exhibits NADP-dependent alcohol dehydrogenase activity, acting on a broad variety of aromatic and aliphatic primary alcohols and aldehydes but not on secondary alcohols or ketones. It exhibits superior catalytic efficiency for aldehyde reduction compared to that for alcohol oxidation. The enzyme is a cytosolic protein present in photoautotrophically grown Synechocystis cells. The expression of AdhA is enhanced upon the exposure of cells to different environmental stresses, although it is not essential for survival even under such stress conditions. The induction of the expression of the adhA gene is dependent on the Hik34-Rre1 two-component system, as it is severely impaired in mutant strains lacking either the histidine kinase Hik34 or the response regulator Rre1. In vitro DNA-protein interaction analysis reveals that the response regulator Rre1 binds specifically to the promoter region of the adhA gene.

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