scholarly journals The Response Regulator Slr1588 Regulates spsA But Is Not Crucial for Salt Acclimation of Synechocystis sp. PCC 6803

2017 ◽  
Vol 8 ◽  
Author(s):  
Kuo Song ◽  
Martin Hagemann ◽  
Xiaoming Tan ◽  
Xuefeng Lu
Keyword(s):  
Response Regulator ◽  
Salt Acclimation ◽  
Synechocystis Sp ◽  
Pcc 6803

Elucidating butanol tolerance mediated by a response regulator Sll0039 in Synechocystis sp. PCC 6803 using a metabolomic approach

2015 ◽  
Vol 99 (4) ◽  
pp. 1845-1857 ◽  
Author(s):  
Xiangfeng Niu ◽  
Ye Zhu ◽  
Guangsheng Pei ◽  
Lina Wu ◽  
Lei Chen ◽  
...  
Keyword(s):  
Response Regulator ◽  
Butanol Tolerance ◽  
Metabolomic Approach ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Slr0967 and Sll0939 induced by the SphR response regulator in Synechocystis sp. PCC 6803 are essential for growth under acid stress conditions

2012 ◽  
Vol 1817 (8) ◽  
pp. 1270-1276 ◽  
Author(s):  
Junji Uchiyama ◽  
Ryosuke Asakura ◽  
Mayuko Kimura ◽  
Atsushi Moriyama ◽  
Hiroko Tahara ◽  
...  
Keyword(s):  
Response Regulator ◽  
Acid Stress ◽  
Stress Conditions ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Physiological and Molecular Characterization of a Synechocystis sp. PCC 6803 Mutant Lacking Histidine Kinase Slr1759 and Response Regulator Slr1760

2006 ◽  
Vol 61 (11-12) ◽  
pp. 865-878 ◽  
Author(s):  
Anke Nodop ◽  
Iwane Suzuki ◽  
Aiko Barsch ◽  
Ann-Kristin Schröder ◽  
Karsten Niehaus ◽  
...  
Keyword(s):  
Histidine Kinase ◽  
Ph Value ◽  
Response Regulator ◽  
Two Component System ◽  
Component System ◽  
Bg11 Medium ◽  
Two Component ◽  
Metabolic Activities ◽  
Synechocystis Sp ◽  
Pcc 6803

Abstract The hybrid sensory histidine kinase Slr1759 of the cyanobacterium Synechocystis sp. strain PCC 6803 contains multiple sensory domains and a multi-step phosphorelay system. Immuno blot analysis provided evidence that the histidine kinase Slr1759 is associated with the cytoplasmic membrane. The gene slr1759 is part of an operon together with slr1760, encoding a response regulator. A comparative investigation was performed on Synechocystis sp. strain PCC 6803 wild type (WT) and an insertionally inactivated slr1759-mutant (Hik14) which also lacks the transcript for the response regulator Slr1760. The mutant Hik14 grew significantly slower than WT in the early growth phase, when both were inoculated with a low cell density into BG11 medium without additional buffer and when aerated with air enriched with 2% CO2. Since the aeration with CO2-enriched air results in a decrease of the pH value in the medium, the growth experiments indicated that Hik14 is not able to adjust its metabolic activities as rapidly as WT to compensate for a larger decrease of the pH value in the medium. No significant differences in growth between Hik14 and WT were observed when cells were inoculated with a higher cell density in BG11 medium or when the BG11 medium contained 50 mm Epps-NaOH, pH 7.5, to prevent the pH drop. This Hik14 phenotype has so far only been seen under the above defined growth condition. Results of photosynthetic activity measurements as well as Northern blot-, immuno blot-, and metabolite analyses suggest that the two-component system Slr1759/Slr1760 has a function in the coordination of several metabolic activities which is in good agreement with the complex domain structure of Slr1759. The direct targets of this two-component system have so far not been identified.

Perception and transduction of low-temperature signals to induce desaturation of fatty acids

2000 ◽  
Vol 28 (6) ◽  
pp. 628-630 ◽  
Author(s):  
I. Suzuki ◽  
D. A. Los ◽  
N. Murata
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Low Temperature ◽  
Membrane Lipids ◽  
Response Regulator ◽  
Random Mutagenesis ◽  
Fatty Acid Desaturases ◽  
Histidine Kinases ◽  
Synechocystis Sp ◽  
Pcc 6803

When cells of the cyanobacterium Synechocystis sp. PCC 6803 are exposed to a low temperature, genes for fatty acid desaturases are expressed with resultant increases in the degree of unsaturation of fatty acids in membrane lipids. However, the sensor and transducers of low-temperature signals had not yet been identified. In order to identify these components we applied to the cyanobacterium Synechocystis sp. PCC 6803 the systematic disruption of all 43 putative genes for histidine kinases and random mutagenesis of the whole genome in conjunction with screening by the transcriptional activity of the promoter of the desB gene for the ω 3 desaturase. This allowed us to identify two histidine kinases and a response regulator as components of the perception and transduction of low-temperature signals for the expression of genes for fatty acid desaturases.

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