scholarly journals Slr0967 and Sll0939 induced by the SphR response regulator in Synechocystis sp. PCC 6803 are essential for growth under acid stress conditions

2012 ◽  
Vol 1817 (8) ◽  
pp. 1270-1276 ◽  
Author(s):  
Junji Uchiyama ◽  
Ryosuke Asakura ◽  
Mayuko Kimura ◽  
Atsushi Moriyama ◽  
Hiroko Tahara ◽  
...  
Keyword(s):  
Response Regulator ◽  
Acid Stress ◽  
Stress Conditions ◽  
Synechocystis Sp ◽  
Pcc 6803

Elucidating butanol tolerance mediated by a response regulator Sll0039 in Synechocystis sp. PCC 6803 using a metabolomic approach

2015 ◽  
Vol 99 (4) ◽  
pp. 1845-1857 ◽  
Author(s):  
Xiangfeng Niu ◽  
Ye Zhu ◽  
Guangsheng Pei ◽  
Lina Wu ◽  
Lei Chen ◽  
...  
Keyword(s):  
Response Regulator ◽  
Butanol Tolerance ◽  
Metabolomic Approach ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Characterization of an Alcohol Dehydrogenase from the Cyanobacterium Synechocystis sp. Strain PCC 6803 That Responds to Environmental Stress Conditions via the Hik34-Rre1 Two-Component System

2009 ◽  
Vol 191 (13) ◽  
pp. 4383-4391 ◽  
Author(s):  
Rebeca Vidal ◽  
Luis López-Maury ◽  
Miguel G. Guerrero ◽  
Francisco J. Florencio
Keyword(s):  
Alcohol Dehydrogenase ◽  
Interaction Analysis ◽  
Response Regulator ◽  
Stress Conditions ◽  
Primary Alcohols ◽  
Two Component System ◽  
Component System ◽  
Two Component ◽  
Pcc 6803

ABSTRACT The slr1192 (adhA) gene from Synechocystis sp. strain PCC 6803 encodes a member of the medium-chain alcohol dehydrogenase/reductase family. The gene product AdhA exhibits NADP-dependent alcohol dehydrogenase activity, acting on a broad variety of aromatic and aliphatic primary alcohols and aldehydes but not on secondary alcohols or ketones. It exhibits superior catalytic efficiency for aldehyde reduction compared to that for alcohol oxidation. The enzyme is a cytosolic protein present in photoautotrophically grown Synechocystis cells. The expression of AdhA is enhanced upon the exposure of cells to different environmental stresses, although it is not essential for survival even under such stress conditions. The induction of the expression of the adhA gene is dependent on the Hik34-Rre1 two-component system, as it is severely impaired in mutant strains lacking either the histidine kinase Hik34 or the response regulator Rre1. In vitro DNA-protein interaction analysis reveals that the response regulator Rre1 binds specifically to the promoter region of the adhA gene.

Identification of genes expressed in response to acid stress in Synechocystis sp. PCC 6803 using DNA microarrays

2005 ◽  
Vol 84 (1-3) ◽  
pp. 225-230 ◽  
Author(s):  
Hisataka Ohta ◽  
Yousuke Shibata ◽  
Youhei Haseyama ◽  
Yuka Yoshino ◽  
Takehiro Suzuki ◽  
...  
Keyword(s):  
Dna Microarrays ◽  
Acid Stress ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Physiological and Molecular Characterization of a Synechocystis sp. PCC 6803 Mutant Lacking Histidine Kinase Slr1759 and Response Regulator Slr1760

2006 ◽  
Vol 61 (11-12) ◽  
pp. 865-878 ◽  
Author(s):  
Anke Nodop ◽  
Iwane Suzuki ◽  
Aiko Barsch ◽  
Ann-Kristin Schröder ◽  
Karsten Niehaus ◽  
...  
Keyword(s):  
Histidine Kinase ◽  
Ph Value ◽  
Response Regulator ◽  
Two Component System ◽  
Component System ◽  
Bg11 Medium ◽  
Two Component ◽  
Metabolic Activities ◽  
Synechocystis Sp ◽  
Pcc 6803

Abstract The hybrid sensory histidine kinase Slr1759 of the cyanobacterium Synechocystis sp. strain PCC 6803 contains multiple sensory domains and a multi-step phosphorelay system. Immuno blot analysis provided evidence that the histidine kinase Slr1759 is associated with the cytoplasmic membrane. The gene slr1759 is part of an operon together with slr1760, encoding a response regulator. A comparative investigation was performed on Synechocystis sp. strain PCC 6803 wild type (WT) and an insertionally inactivated slr1759-mutant (Hik14) which also lacks the transcript for the response regulator Slr1760. The mutant Hik14 grew significantly slower than WT in the early growth phase, when both were inoculated with a low cell density into BG11 medium without additional buffer and when aerated with air enriched with 2% CO2. Since the aeration with CO2-enriched air results in a decrease of the pH value in the medium, the growth experiments indicated that Hik14 is not able to adjust its metabolic activities as rapidly as WT to compensate for a larger decrease of the pH value in the medium. No significant differences in growth between Hik14 and WT were observed when cells were inoculated with a higher cell density in BG11 medium or when the BG11 medium contained 50 mm Epps-NaOH, pH 7.5, to prevent the pH drop. This Hik14 phenotype has so far only been seen under the above defined growth condition. Results of photosynthetic activity measurements as well as Northern blot-, immuno blot-, and metabolite analyses suggest that the two-component system Slr1759/Slr1760 has a function in the coordination of several metabolic activities which is in good agreement with the complex domain structure of Slr1759. The direct targets of this two-component system have so far not been identified.

Sign in / Sign up

Export Citation Format

Share Document