Proline Accumulation Is Inhibitory to Arabidopsis Seedlings during Heat Stress

Wei-Tao Lv; Bin Lin; Min Zhang; Xue-Jun Hua

doi:10.1104/pp.111.175810

Diurnal gradual heat stress affects antioxidant enzymes, proline accumulation and some physiological components in cotton (Gossypium hirsutum L.)

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb09.1590 ◽

2010 ◽

Vol 9 (7) ◽

pp. 1008-1015 ◽

Cited By ~ 25

Author(s):

Guuml r Atilla ◽

Demirel Ufuk ◽

Ouml zden Mustafa ◽

Kahraman Abdullah ◽

Ccedil opur Osman

Keyword(s):

Antioxidant Enzymes ◽

Heat Stress ◽

Gossypium Hirsutum ◽

Proline Accumulation ◽

Gossypium Hirsutum L

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Genetic Manipulation of Proline Accumulation Influences the Concentrations of Other Amino Acids in Soybean Subjected to Simultaneous Drought and Heat Stress

Journal of Agricultural and Food Chemistry ◽

10.1021/jf050540l ◽

2005 ◽

Vol 53 (19) ◽

pp. 7512-7517 ◽

Cited By ~ 35

Author(s):

Livia Simon-Sarkadi ◽

Gábor Kocsy ◽

Ágnes Várhegyi ◽

Gábor Galiba ◽

Jacoba A. de Ronde

Keyword(s):

Amino Acids ◽

Heat Stress ◽

Genetic Manipulation ◽

Proline Accumulation ◽

Drought And Heat Stress

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Differences in proline accumulation between wheat varieties in response to heat stress

Botanica Serbica ◽

10.2298/botserb2101061d ◽

2021 ◽

Vol 45 (1) ◽

pp. 61-69

Author(s):

Nevena Djukic ◽

Stefan Markovic ◽

Jasna Mastilovic ◽

Predrag Simovic

Keyword(s):

Heat Stress ◽

Starch Content ◽

Photosynthetic Pigment ◽

Wheat Variety ◽

Grain Filling ◽

Proline Accumulation ◽

Wheat Varieties ◽

Photosynthetic Pigment Content ◽

Filling Phase ◽

Proline Concentration

Proline is one of the organic osmolytes that accumulates in plants in response to stressful environmental conditions. The aim of this study was to determine the concentration of proline in the grain filling phase in ten winter wheat cultivars under conditions of prolonged periods of air temperatures above 30?C and to assess the variability of wheat variety responses. The correlation between proline accumulation and photosynthetic pigments, accumulated starch and yield was also determined. In the experimental field, flag leaves were sampled for each variety in the grain filling phase. Statistical data analysis and parameter correlation were performed using the SPSS program. The results showed that there is a statistically significant difference between proline values in moderate and high air temperature conditions when proline accumulation occurs. The significant correlation of accumulated starch content and yield with proline concentration showed that in addition to higher proline values under heat stress, wheat varieties Apac, Talas and Futura also have higher yield and starch content. A correlation was also found between proline concentration and photosynthetic pigment contents, where the Apac wheat variety showed the best adaptive response to the investigated traits and was characterized by a smaller reduction in photosynthetic pigment content under heat stress. The interrelation of proline with the photosynthetic pigment content and wheat quality parameters may be important in breeding technologies aimed at improving wheat stress tolerance.

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Analysis of proline accumulation, antioxidant capacity and HSP expression in mutant rice lines with different heat tolerance

Australian Journal of Crop Science ◽

10.21475/ajcs.21.15.09.sp-2 ◽

2021 ◽

pp. 22-27

Author(s):

Jian-Zhong Huang ◽

Shou-Ling Xu ◽

Ting-Chen Ma ◽

You-Fa Li ◽

Hao-Wei Fu ◽

...

Keyword(s):

Heat Stress ◽

Antioxidant Capacity ◽

Gamma Ray ◽

Oryza Sativa L ◽

Proline Accumulation ◽

Sod Activity ◽

Total Antioxidant ◽

Heat Tolerant ◽

Shock Proteins ◽

Hsp Genes

Three mutant rice (Oryza sativa L.) lines (AG1, AG2 and AG3) were selected as heat tolerant mutants from a gamma-ray-irradiated population of a heat-susceptible line (AG), based on their floret fertility grown under high temperatures. They were subjected to heat stress treatment (45°C, 22 hrs) at the 5-leaf stage, together with a heat-tolerant cultivar N22 and AG. Analysis of seedling root growth by WinRHIZO scanning revealed that N22 and AG3 were more heat-tolerant than the other lines (AG being the most heat susceptible). Following heat stress, a significantly higher level of oxidative damage, as indicated by TBARS, was observed in AG than in N22 and AG3. The proline accumulation was significantly higher in N22 and AG3（12- to 13.5- fold）than AG (2.5- fold). Similarly, significantly greater increases of total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activity were observed in N22 and AG3 than in AG. The expression of four heat shock proteins was also investigated using qPCR: OsHSP16.9, OsHSP80.3 and OsHSP100.9were induced by heat stress to various levels while OsHSP72.6was down-regulated in all tested lines. The heat-induced expression of OsHSP16.9 and OsHSP100.9 in N22 and AG3 was about twice that of AG. Higher proline accumulation and expression of the three HSP genes, as well as elevation ofT-AOC and SOD activity were observed in the heat-tolerant N22 and the mutant line AG3 under heat stress

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Heat-induced accumulation of proline and yield components in genetically divergent cereal varieties

Genetika ◽

10.2298/gensr2101219m ◽

2021 ◽

Vol 53 (1) ◽

pp. 219-233

Author(s):

Stefan Markovic ◽

Desimir Knezevic ◽

Nenad Nesovic ◽

Nevena Djukic

Keyword(s):

Heat Stress ◽

High Temperature ◽

Abiotic Factors ◽

Proline Accumulation ◽

Proline Content ◽

First Year ◽

Wheat Varieties ◽

Fresh Plant ◽

Air Temperatures ◽

Resistant Varieties

Growth and development of cereals is affected by abiotic factors, particularly by high temperature. An important factor in plant adaptation to abiotic stress can be the proline accumulation. Proline is an amino acid involved in a series of metabolic processes and is important as a protein stabilizer, osmolyte and antioxidant. The aim of this experiment was to determine the effect of high temperature on the proline content and yield elements in different cereal varieties during two vegetative seasons. For the investigation, plant material of 8 genetically divergent winter wheat varieties, 1 variety of triticale and 1 oat variety were used. Samples were collected in days with moderate midday air temperatures of 24-26?C in the milk stage and after a few days in the same phenological stage, in conditions of high midday air temperatures of 34-36?C. Proline accumulation was determined spectrophotometrically. Statistical analyses of data were done in the SPSS program. The results during the first experimental year showed that under moderate air temperature conditions proline content was 0.661 ?mol g-1 fresh plant, and in the second experimental year 0.777 ?mol g-1 fresh plant. Under conditions of heat stress during the first year, the content of proline increased to 2.169 ?mol g-1 fresh plant, and in the second experimental year the average content was 2.510 ?mol g-1 fresh plant, which confirms the increase of proline accumulations under heat stress. Compared with other cereal varieties, wheat varieties Zvezdana, Pobeda, Simonida and Avenu were characterized by higher contents of proline in conditions of heat stress in both vegetative seasons. Statistical analysis and correlation of the results of proline accumulation and yield elements showed that more resistant varieties to heat stress conditions have higher yields.

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