scholarly journals Chlorophyll Breakdown in Senescent Arabidopsis Leaves. Characterization of Chlorophyll Catabolites and of Chlorophyll Catabolic Enzymes Involved in the Degreening Reaction

2005 ◽  
Vol 139 (1) ◽  
pp. 52-63 ◽  
Author(s):  
Adriana Pružinská ◽  
Gaby Tanner ◽  
Sylvain Aubry ◽  
Iwona Anders ◽  
Simone Moser ◽  
...  
Keyword(s):  
Catabolic Enzymes ◽  
Chlorophyll Breakdown ◽  
Chlorophyll Catabolites

scholarly journals Cloning and characterization of multiple human polyamine oxidase splice variants that code for isoenzymes with different biochemical characteristics

2002 ◽  
Vol 368 (3) ◽  
pp. 673-677 ◽  
Author(s):  
Tracy MURRAY-STEWART ◽  
Yanlin WANG ◽  
Wendy DEVEREUX ◽  
Robert A. CASERO
Keyword(s):  
Metabolic Pathway ◽  
Splice Variants ◽  
Polyamine Oxidase ◽  
Biochemical Characteristics ◽  
Polyamine Catabolism ◽  
New Class ◽  
Catabolic Enzymes ◽  
Data Support

The recently cloned and characterized human polyamine oxidase (PAOh1) potentially represents a new class of catabolic enzymes in the mammalian polyamine metabolic pathway capable of the efficient oxidation of polyamines. Here the discovery of three additional human PAO splice variants is reported, and the data support the fact that the human PAO gene codes for at least four isoenzymes, each of which exhibit distinctive biochemical characteristics, suggesting the existence of additional levels of complexity in polyamine catabolism.

scholarly journals Structures of Chlorophyll Catabolites in Bananas (Musa acuminata)Reveal a Split Path of Chlorophyll Breakdown in a Ripening Fruit

2012 ◽  
Vol 18 (35) ◽  
pp. 10873-10885 ◽  
Author(s):  
Simone Moser ◽  
Thomas Müller ◽  
Andreas Holzinger ◽  
Cornelius Lütz ◽  
Bernhard Kräutler
Keyword(s):  
Musa Acuminata ◽  
Chlorophyll Breakdown ◽  
Chlorophyll Catabolites

Characterization of nit-2, the major nitrogen regulatory gene of Neurospora crassa

1987 ◽  
Vol 7 (5) ◽  
pp. 1691-1696
Author(s):  
Y H Fu ◽  
G A Marzluf
Keyword(s):  
Neurospora Crassa ◽  
Northern Blot Analysis ◽  
Regulatory Gene ◽  
Catabolic Enzymes ◽  
Dna Fragment ◽  
Autogenous Regulation ◽  
Different Levels ◽  
Group 1 ◽  
In Cells

The nit-2 gene is the major nitrogen-regulatory gene of Neurospora crassa, and under conditions of nitrogen limitations, it turns on the expression of various unlinked structural genes which specify nitrogen-catabolic enzymes. The nit-2 gene was subcloned as a 6-kilobase (kb) DNA fragment from a cosmid that carried approximately a 40-kb N. crassa DNA insert. The nit-2 gene was localized in a DNA segment of approximately 3.5 kb and was shown to correspond to a unique DNA sequence located on linkage group 1. Several N. crassa nit-2 transformants were characterized and were found to possess significantly different levels of the regulated enzyme nitrate reductase. Northern blot analysis of RNA from various strains was carried out to determine whether the nit-2 gene was expressed constitutively or was itself subject to regulation. The results revealed that the nit-2 gene is transcribed to give a single large mRNA of approximately 3.5 kb. Expression of the nit-2 gene is regulated such that its transcript is present at a substantially higher level in cells which are limited for nitrogen than in cells growing under nitrogen-repressed conditions. However, the nit-2 gene is not controlled by autogenous regulation. The nit-2 gene was transcribed only at a low level in nmr-1 and in gln-1b, under both nitrogen-repressed and derepressed conditions, suggesting that these unlinked loci may exert a positive regulatory effect on nit-2.

Chlorophyll Breakdown in Tobacco: On the Structure of Two Nonfluorescent Chlorophyll Catabolites

2004 ◽  
Vol 1 (4) ◽  
pp. 657-668 ◽  
Author(s):  
Joachim Berghold ◽  
Christian Eichmüller ◽  
Stefan Hörtensteiner ◽  
Bernhard Kräutler
Keyword(s):  
Chlorophyll Breakdown ◽  
Chlorophyll Catabolites

Unravelling chlorophyll catabolism in higher plants

2002 ◽  
Vol 30 (4) ◽  
pp. 625-630 ◽  
Author(s):  
B. Kräutler
Keyword(s):  
Higher Plants ◽  
Structural Features ◽  
Spectroscopic Methods ◽  
Chlorophyll Metabolism ◽  
Chlorophyll Breakdown ◽  
Chlorophyll Catabolites

Chlorophyll metabolism is probably the most visible manifestation of life. In spite of this, chlorophyll catabolism has remained something of a mystery until about 10 years ago. At that time, the first non-green tetrapyrrolic chlorophyll breakdown products from higher plants were discovered, and the structure of the first one of them was elucidated by modern spectroscopic methods. In the meantime, the essential structural features of chlorophyll catabolites and some of the biochemistry of chlorophyll breakdown in higher plants have been uncovered, as outlined in this article.

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