scholarly journals Cloning and characterization of multiple human polyamine oxidase splice variants that code for isoenzymes with different biochemical characteristics

2002 ◽  
Vol 368 (3) ◽  
pp. 673-677 ◽  
Author(s):  
Tracy MURRAY-STEWART ◽  
Yanlin WANG ◽  
Wendy DEVEREUX ◽  
Robert A. CASERO
Keyword(s):  
Metabolic Pathway ◽  
Splice Variants ◽  
Polyamine Oxidase ◽  
Biochemical Characteristics ◽  
Polyamine Catabolism ◽  
New Class ◽  
Catabolic Enzymes ◽  
Data Support

The recently cloned and characterized human polyamine oxidase (PAOh1) potentially represents a new class of catabolic enzymes in the mammalian polyamine metabolic pathway capable of the efficient oxidation of polyamines. Here the discovery of three additional human PAO splice variants is reported, and the data support the fact that the human PAO gene codes for at least four isoenzymes, each of which exhibit distinctive biochemical characteristics, suggesting the existence of additional levels of complexity in polyamine catabolism.

scholarly journals Secretion of an Acid Phosphatase (SapM) by Mycobacterium tuberculosis That Is Similar to Eukaryotic Acid Phosphatases

2000 ◽  
Vol 182 (23) ◽  
pp. 6850-6853 ◽  
Author(s):  
Mazen T. Saleh ◽  
John T. Belisle
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Acid Phosphatase ◽  
Culture Filtrate ◽  
Sequence Homology ◽  
Acid Phosphatases ◽  
Mature Protein ◽  
Biochemical Characteristics ◽  
New Class

ABSTRACT Mycobacterium tuberculosis secretes a large number of polypeptides with broad biological and immunological functions. We describe here the characterization of a 28-kDa acid phosphatase ofM. tuberculosis (SapM) localized to the culture filtrate. The mature protein demonstrated biochemical characteristics similar to those of the bacterial nonspecific acid phosphatases. However, SapM yielded significant sequence homology to fungal acid phosphatases and not those of bacteria. Thus, SapM may represent a new class of bacterial nonspecific acid phosphatases.

Targeting IDH Mutations in AML: Wielding the Double-edged Sword of Differentiation

2020 ◽  
Vol 20 (7) ◽  
pp. 490-500 ◽  
Author(s):  
Justin S. Becker ◽  
Amir T. Fathi
Keyword(s):  
Genome Structure ◽  
Cellular Metabolism ◽  
Standard Of Care ◽  
Competitive Inhibitor ◽  
Driver Mutations ◽  
New Class ◽  
Regulatory Enzymes ◽  
Idh Mutations ◽  
Genomic Characterization

The genomic characterization of acute myeloid leukemia (AML) by DNA sequencing has illuminated subclasses of the disease, with distinct driver mutations, that might be responsive to targeted therapies. Approximately 15-23% of AML genomes harbor mutations in one of two isoforms of isocitrate dehydrogenase (IDH1 or IDH2). These enzymes are constitutive mediators of basic cellular metabolism, but their mutated forms in cancer synthesize an abnormal metabolite, 2- hydroxyglutarate, that in turn acts as a competitive inhibitor of multiple gene regulatory enzymes. As a result, leukemic IDH mutations cause changes in genome structure and gene activity, culminating in an arrest of normal myeloid differentiation. These discoveries have motivated the development of a new class of selective small molecules with the ability to inhibit the mutant IDH enzymes while sparing normal cellular metabolism. These agents have shown promising anti-leukemic activity in animal models and early clinical trials, and are now entering Phase 3 study. This review will focus on the growing preclinical and clinical data evaluating IDH inhibitors for the treatment of IDH-mutated AML. These data suggest that inducing cellular differentiation is central to the mechanism of clinical efficacy for IDH inhibitors, while also mediating toxicity for patients who experience IDH Differentiation Syndrome. Ongoing trials are studying the efficacy of IDH inhibitors in combination with other AML therapies, both to evaluate potential synergistic combinations as well as to identify the appropriate place for IDH inhibitors within existing standard-of-care regimens.

Identification and characterization of novel splice variants of human farnesoid X receptor

2021 ◽  
pp. 108893
Author(s):  
Enni-Kaisa Mustonen ◽  
Serene M.L. Lee ◽  
Hanno Nieß ◽  
Matthias Schwab ◽  
Tatu Pantsar ◽  
...  
Keyword(s):  
Splice Variants ◽  
Farnesoid X Receptor ◽  
Identification And Characterization

scholarly journals Characterization of the Antibiotic Compound No. 70 Produced byStreptomycessp. IMV-70

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Lyudmila P. Trenozhnikova ◽  
Almagul K. Khasenova ◽  
Assya S. Balgimbaeva ◽  
Galina B. Fedorova ◽  
Genrikh S. Katrukha ◽  
...  
Keyword(s):  
Optimal Growth ◽  
Growth Conditions ◽  
Culture Broth ◽  
Producer Strain ◽  
New Class ◽  
New Antibiotics ◽  
Actinomycete Strain ◽  
Main Component

We describe the actinomycete strain IMV-70 isolated from the soils of Kazakhstan, which produces potent antibiotics with high levels of antibacterial activity. After the research of its morphological, chemotaxonomic, and cultural characteristics, the strain with potential to be developed further as a novel class of antibiotics with chemotherapeutics potential was identified asStreptomycessp. IMV-70. In the process of fermentation, the strainStreptomycesspp. IMV-70 produces the antibiotic no. 70, which was isolated from the culture broth by extraction with organic solvents. Antibiotic compound no. 70 was purified and separated into individual components by HPLC, TLC, and column chromatography methods. The main component of the compound is the antibiotic 70-A, which was found to be identical to the peptolide etamycin A. Two other antibiotics 70-B and 70-C have never been described and therefore are new antibiotics. The physical-chemical and biological characteristics of these preparations were described and further researched. Determination of the optimal growth conditions to cultivate actinomycete-producer strain IMV-70 and development of methods to isolate, purify, and accumulate preparations of the new antibiotic no. 70 enable us to research further the potential of this new class of antibiotics.

HIGH DIMENSIONAL KNOT GROUPS AND HNN EXTENSIONS OF THE FIBONACCI GROUPS

1998 ◽  
Vol 07 (04) ◽  
pp. 503-508 ◽  
Author(s):  
ANDRZEJ SZCZEPAŃSKI
Keyword(s):  
High Dimensional ◽  
New Class ◽  
Hnn Extensions

We shall present a new class of examples of high dimensional knot groups. All of them are HNN extensions of the Fibonacci groups. We give also some characterization of these groups.

Characterization of a new class of deletions of the D region of the bacteriophage T4 genome

Virology ◽  
1975 ◽  
Vol 64 (1) ◽  
pp. 144-152 ◽  
Author(s):  
Richard E. Depew ◽  
Thomas J. Snopek ◽  
Nicholas R. Cozzarelli
Keyword(s):  
Bacteriophage T4 ◽  
New Class ◽  
D Region
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