scholarly journals Characterization of the Lotus japonicus Symbiotic Mutant lot1 That Shows a Reduced Nodule Number and Distorted Trichomes

2005 ◽  
Vol 137 (4) ◽  
pp. 1261-1271 ◽  
Author(s):  
Yasuhiro Ooki ◽  
Mari Banba ◽  
Koji Yano ◽  
Jumpei Maruya ◽  
Shusei Sato ◽  
...  
Keyword(s):  
Lotus Japonicus ◽  
Nodule Number

scholarly journals Chromosomal Map of the Model LegumeLotus japonicus

Genetics ◽  
2002 ◽  
Vol 161 (4) ◽  
pp. 1661-1672 ◽  
Author(s):  
Andrea Pedrosa ◽  
Niels Sandal ◽  
Jens Stougaard ◽  
Dieter Schweizer ◽  
Andreas Bachmair
Keyword(s):  
Repetitive Sequences ◽  
Lotus Japonicus ◽  
Chromosome Complement ◽  
Linkage Groups ◽  
Closely Related Species ◽  
F1 Hybrid ◽  
Bac Clones ◽  
Genomic Regions ◽  
Chromosomal Map

AbstractLotus japonicus is a model plant for the legume family. To facilitate map-based cloning approaches and genome analysis, we performed an extensive characterization of the chromosome complement of the species. A detailed karyotype of L. japonicus Gifu was built and plasmid and BAC clones, corresponding to genetically mapped markers (see the accompanying article by Sandal  et al. 2002, this issue), were used for FISH to correlate genetic and chromosomal maps. Hybridization of DNA clones from 32 different genomic regions enabled the assignment of linkage groups to chromosomes, the comparison between genetic and physical distances throughout the genome, and the partial characterization of different repetitive sequences, including telomeric and centromeric repeats. Additional analysis of L. filicaulis and its F1 hybrid with L. japonicus demonstrated the occurrence of inversions between these closely related species, suggesting that these chromosome rearrangements are early events in speciation of this group.

scholarly journals Isolation and molecular characterization of a Lotus japonicus R2R3-MYB subgroup 7 transcription factor gene

2017 ◽  
Vol 34 (1) ◽  
pp. 45-49 ◽  
Author(s):  
Satoru Kunihiro ◽  
Daigo Tanabe ◽  
Yuiko Niwa ◽  
Keisuke Kitamura ◽  
Jun Abe ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Molecular Characterization ◽  
Lotus Japonicus ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
R2r3 Myb

scholarly journals MIR2111-5 locus and shoot-accumulated mature miR2111 systemically enhance nodulation depending on HAR1 in Lotus japonicus

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Nao Okuma ◽  
Takashi Soyano ◽  
Takuya Suzaki ◽  
Masayoshi Kawaguchi
Keyword(s):  
Root Nodules ◽  
Lotus Japonicus ◽  
Dependent Manner ◽  
Rna Seq ◽  
Signaling System ◽  
Nodule Number ◽  
Knockout Mutants ◽  
Mutualistic Relationship ◽  
Dose Dependent

Abstract Legumes utilize a shoot-mediated signaling system to maintain a mutualistic relationship with nitrogen-fixing bacteria in root nodules. In Lotus japonicus, shoot-to-root transfer of microRNA miR2111 that targets TOO MUCH LOVE, a nodulation suppressor in roots, has been proposed to explain the mechanism underlying nodulation control from shoots. However, the role of shoot-accumulating miR2111s for the systemic regulation of nodulation was not clearly shown. Here, we find L. japonicus has seven miR2111 loci, including those mapped through RNA-seq. MIR2111-5 expression in leaves is the highest among miR2111 loci and repressed after rhizobial infection depending on a shoot-acting HYPERNODULATION ABERRANT ROOT FORMATION1 (HAR1) receptor. MIR2111-5 knockout mutants show significantly decreased nodule numbers and miR2111 levels. Furthermore, grafting experiments using transformants demonstrate scions with altered miR2111 levels influence nodule numbers in rootstocks in a dose-dependent manner. Therefore, miR2111 accumulation in leaves through MIR2111-5 expression is required for HAR1-dependent systemic optimization of nodule number.

scholarly journals Flavonoids and Isoflavonoids Biosynthesis in the Model Legume Lotus japonicus; Connections to Nitrogen Metabolism and Photorespiration

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 774 ◽  
Author(s):  
Margarita García-Calderón ◽  
Carmen M. Pérez-Delgado ◽  
Peter Palove-Balang ◽  
Marco Betti ◽  
Antonio J. Márquez
Keyword(s):  
Abiotic Stress ◽  
Nitrogen Metabolism ◽  
Lotus Japonicus ◽  
Regulatory Proteins ◽  
Phenylpropanoid Metabolism ◽  
Biological Processes ◽  
Model Legume ◽  
Different Types ◽  
Made In

Phenylpropanoid metabolism represents an important metabolic pathway from which originates a wide number of secondary metabolites derived from phenylalanine or tyrosine, such as flavonoids and isoflavonoids, crucial molecules in plants implicated in a large number of biological processes. Therefore, various types of interconnection exist between different aspects of nitrogen metabolism and the biosynthesis of these compounds. For legumes, flavonoids and isoflavonoids are postulated to play pivotal roles in adaptation to their biological environments, both as defensive compounds (phytoalexins) and as chemical signals in symbiotic nitrogen fixation with rhizobia. In this paper, we summarize the recent progress made in the characterization of flavonoid and isoflavonoid biosynthetic pathways in the model legume Lotus japonicus (Regel) Larsen under different abiotic stress situations, such as drought, the impairment of photorespiration and UV-B irradiation. Emphasis is placed on results obtained using photorespiratory mutants deficient in glutamine synthetase. The results provide different types of evidence showing that an enhancement of isoflavonoid compared to standard flavonol metabolism frequently occurs in Lotus under abiotic stress conditions. The advance produced in the analysis of isoflavonoid regulatory proteins by the use of co-expression networks, particularly MYB transcription factors, is also described. The results obtained in Lotus japonicus plants can be also extrapolated to other cultivated legume species, such as soybean, of extraordinary agronomic importance with a high impact in feeding, oil production and human health.

scholarly journals Genomewide Characterization of the Light-Responsive and Clock-Controlled Output Pathways in Lotus japonicus with Special Emphasis of its Uniqueness

2010 ◽  
Vol 51 (10) ◽  
pp. 1800-1814 ◽  
Author(s):  
Natsuko Ono ◽  
Kai Ishida ◽  
Takafumi Yamashino ◽  
Hanayo Nakanishi ◽  
Shusei Sato ◽  
...  
Keyword(s):  
Lotus Japonicus

scholarly journals Root, Root Hair, and Symbiotic Mutants of the Model Legume Lotus japonicus

2002 ◽  
Vol 15 (1) ◽  
pp. 17-26 ◽  
Author(s):  
Masayoshi Kawaguchi ◽  
Haruko Imaizumi-Anraku ◽  
Hiroyuki Koiwa ◽  
Sinobu Niwa ◽  
Akira Ikuta ◽  
...  
Keyword(s):  
Root Hair ◽  
Phenotypic Variation ◽  
Developmental Process ◽  
Lotus Japonicus ◽  
Root Hairs ◽  
Model Legume ◽  
Nodule Number ◽  
Stable Mutant ◽  
Mutant Lines ◽  
Hypernodulating Mutants

To gain an overview of plant factors controlling nodule number and organogenesis, an extensive screening using model legume Lotus japonicus was carried out. This screening involved 40,000 M2 seeds, and 32 stable mutant lines were isolated. From these, 16 mutant lines maintaining the phenotypic variation were selected and genetically analyzed. With respect to nodule number, four loci were identified, Ljsym77, Ljsym78, slippery root (slp), and radial organization1 (rdo1). The former two mutants have an increased number of nodules, while the latter two have a decreased number. Ljsym78-1 and Ljsym78-2 are hypernodulating mutants with a branched root system and were found to be allelic to Ljsym16. The phenotype of the Ljsym77 mutant was highly pleiotropic, being deficient in light and gravity responses. The slp mutant was isolated as a low-nodulating mutant lacking root hairs. Concerning nodule organogenesis, nine symbiotic loci were identified, including the two loci alb1 and fen1. Mutants affecting the developmental process of nodule organogenesis were placed in three phenotypic categories: Nod¯ (Ljsym70 to Ljsym73), Hist¯ (alb1-1, alb1-2, and Ljsym79), and Fix¯ (fen1, Ljsym75, and Ljsym81).

scholarly journals Rapid characterization of plant mutants with an altered ion-profile: a case study using Lotus japonicus

2008 ◽  
Vol 181 (4) ◽  
pp. 795-801 ◽  
Author(s):  
Zheng Chen ◽  
Toshihiro Watanabe ◽  
Takuro Shinano ◽  
Keiki Okazaki ◽  
Mitsuru Osaki
Keyword(s):  
Lotus Japonicus ◽  
Rapid Characterization

scholarly journals Characterization of a Developmental Root Response Caused by External Ammonium Supply in Lotus japonicus

2010 ◽  
Vol 154 (2) ◽  
pp. 784-795 ◽  
Author(s):  
Alessandra Rogato ◽  
Enrica D’Apuzzo ◽  
Ani Barbulova ◽  
Selim Omrane ◽  
Aurora Parlati ◽  
...  
Keyword(s):  
Lotus Japonicus ◽  
Root Response

Functional characterization of an ammonium transporter gene from Lotus japonicus

Gene ◽  
2001 ◽  
Vol 270 (1-2) ◽  
pp. 237-243 ◽  
Author(s):  
Francesca Salvemini ◽  
Anne-Marie Marini ◽  
Anna Riccio ◽  
Eduardo J Patriarca ◽  
Maurizio Chiurazzi
Keyword(s):  
Functional Characterization ◽  
Lotus Japonicus ◽  
Ammonium Transporter ◽  
Transporter Gene

Expression and characterization of PKL01, an Ndr kinase homolog in Lotus japonicus

2010 ◽  
Vol 147 (6) ◽  
pp. 799-807 ◽  
Author(s):  
I. Kameshita ◽  
S. Shimomura ◽  
K. Nishio ◽  
N. Sueyoshi ◽  
T. Nishida ◽  
...  
Keyword(s):  
Lotus Japonicus ◽  
Ndr Kinase
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