scholarly journals An in Vitro System of Indole-3-Acetic Acid Formation from Tryptophan in Maize (Zea mays) Coleoptile Extracts

1993 ◽  
Vol 102 (4) ◽  
pp. 1319-1324 ◽  
Author(s):  
T. Koshiba ◽  
H. Matsuyama
Keyword(s):  
Zea Mays ◽  
Acetic Acid ◽  
Acid Formation ◽  
In Vitro System ◽  
Acetic Acid Formation ◽  
Indole 3 Acetic Acid

Relationship among lipoperoxides, jasmonates and indole-3-acetic acid formation in potato tuber after wounding

2005 ◽  
Vol 39 (6) ◽  
pp. 637-647 ◽  
Author(s):  
Massimo Reverberi ◽  
Corrado Fanelli ◽  
Slaven Zjalic ◽  
Stefania Briganti ◽  
Mauro Picardo ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Potato Tuber ◽  
Acid Formation ◽  
Acetic Acid Formation ◽  
Indole 3 Acetic Acid

scholarly journals In vitro Tomato Fruit Cultures Demonstrate a Role for Indole-3-acetic Acid in Regulating Fruit Ripening

1996 ◽  
Vol 121 (3) ◽  
pp. 520-524 ◽  
Author(s):  
Jerry D. Cohen
Keyword(s):  
Acetic Acid ◽  
Lycopersicon Esculentum ◽  
Fruit Ripening ◽  
Culture Media ◽  
Tomato Fruit ◽  
In Vitro System ◽  
Auxin Conjugates ◽  
Time Period ◽  
Indole 3 Acetic Acid

An in vitro system was used for the production of tomato (Lycopersicon esculentum) fruit in culture starting from immature flowers. This system produced small parthenocarpic (seedless) fruit in response to 10-4m indole-3-acetic acid (IAA) supplied in the medium. Other auxins, auxin conjugates and antiauxins tested were not effective or produced markedly fewer fruit. Additional IAA supplied to the fruit culture media before breaker stage resulted in an increase in the time period between breaker and red-ripe stages from 7 days without additional IAA to 12 days when 10-5m IAA was added. These results suggest that significant changes in the ripening period could be obtained by alteration of auxin relationships in tomato fruit.

scholarly journals The ectopic expression of Arabidopsis glucosyltransferase UGT74D1 affects leaf positioning through modulating indole-3-acetic acid homeostasis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shanghui Jin ◽  
Bingkai Hou ◽  
Guizhi Zhang
Keyword(s):  
Acetic Acid ◽  
Ectopic Expression ◽  
Leaf Angle ◽  
Kinase Substrate ◽  
Auxin Distribution ◽  
Leaf Tissues ◽  
Photosynthesis Efficiency ◽  
Indole 3 Acetic Acid

AbstractLeaf angle is an important agronomic trait affecting photosynthesis efficiency and crop yield. Although the mechanisms involved in the leaf angle control are intensively studied in monocots, factors contribute to the leaf angle in dicots are largely unknown. In this article, we explored the physiological roles of an Arabidopsis glucosyltransferase, UGT74D1, which have been proved to be indole-3-acetic acid (IAA) glucosyltransferase in vitro. We found that UGT74D1 possessed the enzymatic activity toward IAA glucosylation in vivo and its expression was induced by auxins. The ectopically expressed UGT74D1 obviously reduced the leaf angle with an altered IAA level, auxin distribution and cell size in leaf tissues. The expression of several key genes involved in the leaf shaping and leaf positioning, including PHYTOCHROME KINASE SUBSTRATE (PKS) genes and TEOSINTE BRANCHED1, CYCLOIDEA, and PCF (TCP) genes, were dramatically changed by ectopic expression of UGT74D1. In addition, clear transcription changes of YUCCA genes and other auxin related genes can be observed in overexpression lines. Taken together, our data indicate that glucosyltransferase UGT74D1 could affect leaf positioning through modulating auxin homeostasis and regulating transcription of PKS and TCP genes, suggesting a potential new role of UGT74D1 in regulation of leaf angle in dicot Arabidopsis.

scholarly journals Studies on the Growth and Indole-3-Acetic Acid and Abscisic Acid Content of Zea mays Seedlings Grown in Microgravity

1992 ◽  
Vol 100 (2) ◽  
pp. 692-698 ◽  
Author(s):  
Aga Schulze ◽  
Philip J. Jensen ◽  
Mark Desrosiers ◽  
J. George Buta ◽  
Robert S. Bandurski
Keyword(s):  
Zea Mays ◽  
Acetic Acid ◽  
Abscisic Acid ◽  
Acid Content ◽  
Abscisic Acid Content ◽  
Indole 3 Acetic Acid

scholarly journals Effects of Variations in Hormonal Treatments Upon In vitro Regeneration Potential in Embryo Explants of Arenga pinnata

2021 ◽  
Vol 17 (5) ◽  
pp. 495-503
Author(s):  
Shamsiah Abdullah ◽  
Siti Nurain Roslan
Keyword(s):  
Acetic Acid ◽  
In Vitro Regeneration ◽  
Hormonal Treatment ◽  
Height Increment ◽  
Regeneration Potential ◽  
In Vitro Method ◽  
Indole 3 Acetic Acid ◽  
Hormonal Treatments

One of the challenges related to propagation of Arenga pinnata is its lengthy period of seed dormancy. In this study, in vitro regeneration was carried out to determine the effect of hormonal treatment on the embryo explant of Arenga pinnata. Embryos were surface sterilized and cultured into different media supplemented with various hormones concentrations and combinations. Each treatment contained of Kinetin (KN) hormone (1.0, 2.0, and 3.0 mg/l) and in combination with indole-3-acetic acid (IAA) of 0.1, 0.2, 0.3 mg/l. The height of plumule and length of radical was observed and recorded. Treatment 8 (3 mg/ml KN + 0.1 mg/ml IAA) showed 59.09% in plumule height increment while treatment 4 (1 mg/ml KN + 0.3 mg/ml IAA) showed the highest radical increments with 93.62%. The knowledge gained in this study consequently helps us to better understand the role of KN and IAA in the in vitro regeneration protocol. Since in vitro method able to produce higher number of in vitro seedlings at one time, it is important to establish the in vitro regeneration protocol for this plant.

Characterization of the indole-3-acetic acid (IAA) biosynthetic pathway in an epiphytic strain of Erwinia herbicola and IAA production in vitro

1996 ◽  
Vol 42 (6) ◽  
pp. 586-592 ◽  
Author(s):  
M. Brandi ◽  
E. M. Clark ◽  
S. E. Lindow
Keyword(s):  
Acetic Acid ◽  
Pseudomonas Syringae ◽  
Pyruvic Acid ◽  
Enzyme Assays ◽  
Erwinia Herbicola ◽  
Iaa Production ◽  
Acid Pathway ◽  
Culture Supernatants ◽  
Indole 3 Acetic Acid

An epiphytic strain of Erwinia herbicola (strain 299R) synthesized indole-3-acetic acid (IAA) from indole-3-pyruvic acid and indole-3-acetaldehyde, but not from indole-3-acetamide and other intermediates of various IAA biosynthetic pathways in enzyme assays. TLC, HPLC, and GC–MS analyses revealed the presence of indole-3-pyruvic acid, indole-3-ethanol, and IAA in culture supernatants of strain 299R. Indole-3-acetaldehyde was detected in enzyme assays. Furthermore, strain 299R genomic DNA shared no homology with the iaaM and iaaH genes from Pseudomonas syringae pv. savastanoi, even in Southern hybridizations performed under low-stringency conditions. These observations strongly suggest that unlike gall-forming bacteria which can synthesize IAA by indole-3-acetamide, the indole-3-pyruvic acid pathway is the primary route for IAA biosynthesis in this plant-associated strain. IAA synthesis in tryptophan-supplemented cultures of strain 299R was over 10-fold higher under nitrogen-limiting conditions, indicating a possible role for IAA production by bacterial epiphytes in the acquisition of nutrients during growth in their natural habitat.Key words: indole-3-acetic acid, Erwinia, tryptophan, indole-3-pyruvic acid, nitrogen.

scholarly journals The catalytic and radical mechanism for ethanol oxidation to acetic acid

2019 ◽  
Vol 55 (79) ◽  
pp. 11833-11836 ◽  
Author(s):  
Sotiria Mostrou ◽  
Andreas Nagl ◽  
Marco Ranocchiari ◽  
Karin Föttinger ◽  
Jeroen A. van Bokhoven
Keyword(s):  
Acetic Acid ◽  
Liquid Phase ◽  
Ethanol Oxidation ◽  
Acid Formation ◽  
Radical Mechanism ◽  
Acid Proceeds ◽  
Acetic Acid Formation

Liquid-phase ethanol oxidation to acetic acid proceeds in two steps: catalytic ethanol oxidation and non-catalytic acetaldehyde autoxidation. The balance between the two-steps coincides in the acetic acid formation and provides information for designing selective catalysts.

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