Complete Synthetic Medium without Leucine and containing Adenine (CS + A.1-L)

2017 ◽  
Vol 2017 (2) ◽  
pp. pdb.rec090373
Keyword(s):  
Synthetic Medium ◽  
Complete Synthetic Medium

Enrichment of bacteriorhodopsin with isotopically labeled amino acids by biosynthetic incorporation in Halobacterium halobium

1992 ◽  
Vol 38 (11) ◽  
pp. 1181-1185 ◽  
Author(s):  
S. L. Helgerson ◽  
S. L. Siemsen ◽  
E. A. Dratz
Keyword(s):  
Amino Acids ◽  
Synthetic Medium ◽  
Optimal Growth ◽  
Isotopic Labeling ◽  
Maximum Growth ◽  
Integral Membrane Protein ◽  
Maximum Growth Rate ◽  
Halobacterium Halobium ◽  
Isoleucine Leucine ◽  
Complete Synthetic Medium

The growth of Halobacterium halobium was optimized in a chemically defined synthetic medium. Arginine, isoleucine, leucine, lysine, methionine, tyrosine, and valine were found to be essential for growth. Optimal growth rates and cell yields were obtained when the medium was also supplemented with the nonessential amino acids alanine, asparagine, glutamic acid, glycine, phenylalanine, proline, serine, and threonine. The complete synthetic medium supported the same maximum growth rate, cell yield, and production of the integral membrane protein bacteriorhodopsin as was obtained in a complex peptone-based growth medium. Using this defined synthetic medium, isotopically labeled bacteriorhodopsin was produced with several 13C-enriched amino acids. The yield of 13C-labeled bacteriorhodopsin was greater than 35 milligrams of purified protein per litre of cell culture. Key words: bacteriorhodopsin, biosynthetic isotopic labeling, synthetic culture medium, nuclear magnetic resonance.

THE ROLE OF A PROTEASE IN SPORULATION OF PENICILLIUM JANTHINELLUM

1966 ◽  
Vol 44 (5) ◽  
pp. 579-584 ◽  
Author(s):  
A. Thangamani ◽  
T. Hofmann
Keyword(s):  
Acridine Orange ◽  
Synthetic Medium ◽  
Acid Protease ◽  
Logarithmic Phase ◽  
Penicillium Janthinellum ◽  
Indirect Connection ◽  
High Enzyme ◽  
Complete Synthetic Medium ◽  
Enzyme Formation

Although peptidase A, the trypsinogen-activating acid protease of Penicillium janthinellum, is formed during the post-logarithmic phase of growth and while the organism is actively sporulating, there is no direct correlation between the production of the enzyme and sporulation. The evidence for this is threefold: (a) in surface cultures acridine orange almost completely suppresses enzyme formation but reduces sporulation to a much smaller extent; (b) 6-ethyl-thiopurine decreases spore formation but stimulates the production of peptidase A; (c) transfer of mycelium in the log phase from a complete, synthetic medium to a medium lacking nitrate induces the formation of high enzyme levels without affecting the spore count.The possibility of an indirect connection between sporulation and peptidase production is not ruled out.

Components in brain heart infusion selective for chromogenic variants of Staphylococcus aureus

1970 ◽  
Vol 16 (2) ◽  
pp. 91-94 ◽  
Author(s):  
Joseph T. Parisi ◽  
Michael P. Kiley
Keyword(s):  
Amino Acids ◽  
Staphylococcus Aureus ◽  
Amino Acid ◽  
Chromatographic Analysis ◽  
Synthetic Medium ◽  
Brain Heart Infusion ◽  
Large Numbers ◽  
Complete Synthetic Medium ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Large numbers of chromogenic variants were isolated from cultures of a parent strain of Staphylococcus aureus growing in the dialysate but not in the residue of brain heart infusion (Difco). Gas–liquid chromatographic analysis of the dialysate detected 18 amino acids in this medium. Large numbers of chromogenic variants also were isolated from 13 of 18 synthetic media deficient in a single amino acid but not in the complete synthetic medium containing all 18 amino acids. Gas–liquid chromatographic analysis detected marked quantitative differences in the amino acid metabolites present in a complete synthetic medium and the synthetic medium deficient in arginine after growth for 12 days. The data suggest that differences in the amino acid metabolism of the parent and chromogenic variants could account for the population changes observed in brain heart infusion.

STUDIES ON THE NUTRITION OF PHYTOPHTHORA CRYPTOGEA

1961 ◽  
Vol 7 (1) ◽  
pp. 15-25 ◽  
Author(s):  
D. C. Erwin ◽  
H. Katznelson
Keyword(s):  
Synthetic Medium ◽  
Soluble Starch ◽  
Soil Extract ◽  
Root Extract ◽  
Phytophthora Cryptogea ◽  
Low Concentrations ◽  
Wide Range ◽  
Complete Synthetic Medium ◽  
Minimal Concentration ◽  
Alfalfa Root

Phytophthora cryptogea, cause of a root disease of alfalfa, grew well over a wide range of pH values (5.6–7.2) on a synthetic medium containing sucrose as the carbon source. The fungus also grew well on glucose and soluble starch. L-Asparagine was the most favorable source of nitrogen, but failed to support growth of a variant obtained from a single germinated oospore. Low concentrations of CaCl2∙2H2O stimulated growth of P. cryptogea, P. drcehsleri, P. parasitica, and P. boehmeriae. The minimal concentration of thiamine for growth was between 12 and 25 μg/liter. Soil extract, representing 50% or 90% of the volume of the synthetic medium, did not supply the thiamine requirement or stimulate growth of the fungus. Alfalfa root extract, alone or added to the synthetic medium, not only satisfied the thiamine requirement of the fungus but produced about 30% more mycelial growth than the complete synthetic medium.

scholarly journals LATENT VIRAL INFECTION OF CELLS IN TISSUE CULTURE

1958 ◽  
Vol 108 (5) ◽  
pp. 617-630 ◽  
Author(s):  
John P. Bader ◽  
Herbert R. Morgan
Keyword(s):  
Amino Acids ◽  
Viral Infections ◽  
Synthetic Medium ◽  
Water Soluble ◽  
Inorganic Salts ◽  
Virus Propagation ◽  
Virus Growth ◽  
Latent Viral Infection ◽  
Complete Synthetic Medium ◽  
Stimulation Of

Mouse fibroblasts (L cells) fail to support the growth of psittacosis virus (6BC strain) if they are maintained on a medium containing only inorganic salts and glucose for 2 days prior to infection. Virus propagation can be stimulated by the addition of a synthetic medium containing amino acids, water-soluble vitamins, glutamine, glucose, and inorganic salts. By omitting single amino acids from the complete synthetic medium, tyrosine, threonine, methionine, isoleucine, phenylalanine, tryptophan, leucine, valine, and cysteine or cystine were found to be essential for stimulation, while lysine, arginine, histidine, hydroxyproline, proline, glutamic acid, aspartic acid, serine, alanine, and glycine were not essential. The cells on deficient media showed varying degrees of degenerative changes, but there was little correlation between ability to support psittacosis virus growth and morphologic condition of the cells. Glucose is also an essential component of the medium for viral growth, but the absence of glutamine had no effect on stimulation of virus propagation. L cell cultures maintained on media deficient in phenylalanine or tryptophan for 2 days before infection were also found to be incapable of supporting virus growth. The implications of this study in latent viral infections are discussed.

Heterogeneity of Size and Distribution of Intramembrane Particles in the Plasma Membrane of Yeast

1977 ◽  
Vol 35 ◽  
pp. 596-597
Author(s):  
E. Keyhani
Keyword(s):  
Plasma Membrane ◽  
Candida Utilis ◽  
Synthetic Medium ◽  
Freeze Fracture ◽  
Translational Diffusion ◽  
Yeast Cells ◽  
Distilled Water ◽  
Intramembrane Particles ◽  
The Matrix ◽  
Water Cell

The matrix of biological membranes consists of a lipid bilayer into which proteins or protein aggregates are intercalated. Freeze-fracture techni- ques permit these proteins, perhaps in association with lipids, to be visualized in the hydrophobic regions of the membrane. Thus, numerous intramembrane particles (IMP) have been found on the fracture faces of membranes from a wide variety of cells (1-3). A recognized property of IMP is their tendency to form aggregates in response to changes in experi- mental conditions (4,5), perhaps as a result of translational diffusion through the viscous plane of the membrane. The purpose of this communica- tion is to describe the distribution and size of IMP in the plasma membrane of yeast (Candida utilis).Yeast cells (ATCC 8205) were grown in synthetic medium (6), and then harvested after 16 hours of culture, and washed twice in distilled water. Cell pellets were suspended in growth medium supplemented with 30% glycerol and incubated for 30 minutes at 0°C, centrifuged, and prepared for freeze-fracture, as described earlier (2,3).

Performance of Different Solid and Liquid Culture Media for the Improvement of Tuberculin Production

2020 ◽  
Keyword(s):  
Culture Media ◽  
Synthetic Medium ◽  
Chemical Properties ◽  
Economic Loss ◽  
Delayed Type Hypersensitivity ◽  
Basic Medium ◽  
Primary Methods ◽  
Lowenstein Jensen ◽  
Time Required ◽  
Modified Media

Tuberculosis (TB) is one of the most important zoonotic bacterial diseases. A huge economic loss which could be direct or indirect are associated with the disease. Currently, the primary methods used for detection of TB in humans and cattle include the measurement of a delayed type hypersensitivity to purified protein derivative (PPD). So, the need for preparation of purified PPD with adequate properties and increasing the final PPD yield with decreasing the time of tuberculin production has stimulated the interest in the development of its preparation. Our study was performed to compare between the standard and modified media for improving tuberculin production. Middle brook 7H10 agar medium was used as a modified basic medium for mycobacterial growth, followed by cultivation of mycobacteria on Middle brook 7H9 broth medium. For the production, strains were inoculated onto the culture medium (Dorest Henly synthetic medium). Other steps for tuberculin production was done according to standard Weighbridge protocol. The results demonstrated that the using of both Middle brook 7H10 agar and Middle brook 7H9 broth instead of Lowenstein-Jensen (LJ) and glycerin broth media which used in currently produced tuberculin, have better physical and chemical properties. In addition, reducing the time required for production by accelerating the time of microbial growth. Also, it was found that the tuberculin produced using modified media was slightly more potent or the same as currently tuberculin produced. So, both Middle brook 7H10 agar and Middle brook 7H9 broth media are recommended for production of tuberculin saving time and increasing potency of the product but more investigation was recommended for estimation types of protein present in both locally prepared and modified tuberculin.

Activated carbon as a better habitat for water and wastewater treatment microorganisms

2000 ◽  
Vol 42 (12) ◽  
pp. 149-154 ◽  
Author(s):  
M. Okada ◽  
H. Morinaga ◽  
W. Nishijima
Keyword(s):  
Batch Culture ◽  
Bacterial Growth ◽  
Membrane Filter ◽  
Synthetic Medium ◽  
Dialysis Tubing ◽  
Water And Wastewater Treatment ◽  
Batch Cultures ◽  
K 12 ◽  
Bacterial Yield ◽  
Adsorption Desorption

Effects of PAC on bacterial activity were evaluated by sequencing batch cultures (20 hours each) of E.coli K-12 on synthetic medium containing glucose as a sole carbon source. Four suspended sequencing batch culture systems were operated; CP: cultures supplemented with PAC, CR: cultures with removal of metabolites by PAC at the end of each batch culture, CD: cultures supplemented with PAC in dialysis tubing to separate from E.coli, and CC: cultures without PAC (control). The supernatant of each batch culture was filtered through a membrane filter (0.2 μm) and was mixed with the same volume of fresh medium to be used as the medium for the next batch culture. The sequencing batch cultures were repeated three times for all the systems. The bacterial growth in CC was inhibited with the increase in the number of batch cultures. Although a significant amount of metabolites was accumulated in the 3rd batch culture of CC, little accumulation was noted in the 3rd batch culture of CP. No growth inhibition was noted in CP for all the batch cultures. The little differences in the bacterial yield and metabolite accumulation between CR and CD suggested that adsorption/desorption of metabolites with PAC did not play a major role in bacterial growth. PAC addition may partly stimulate the growth by the removal of growth inhibiting metabolites. However, the fact that CP showed higher yield than CR and CD indicated that the contact between bacteria and PAC plays a significant role in the growth of bacteria.

scholarly journals Isolation of Rhodocyclus gelatinosus from poultry slaughterhouse wastewater

2002 ◽  
Vol 45 (4) ◽  
pp. 445-449 ◽  
Author(s):  
Elisa Helena Giglio Ponsano ◽  
Pedro Magalhães Lacava ◽  
Marcos Franke Pinto
Keyword(s):  
Carbon Source ◽  
Photosynthetic Bacteria ◽  
Synthetic Medium ◽  
Poultry Feed ◽  
Gram Negative ◽  
Slaughterhouse Wastewater ◽  
Rhodocyclus Gelatinosus

Four cultures of photosynthetic bacteria isolated from poultry slaughterhouse wastewater were identified as Rhodocyclus gelatinosus based on the following properties: reddish color of cultures in synthetic medium, presence of motility, slightly curved Gram-negative rods morphology, gelatin liquefying activity, utilization of citrate as carbon source and production of bacteriochlorophyl a and carotenoids of the spirilloxanthin alternative series. R. gelatinosus may represent a source of nutrients and pigments with application in poultry feed.

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