THE ROLE OF A PROTEASE IN SPORULATION OF PENICILLIUM JANTHINELLUM

1966 ◽  
Vol 44 (5) ◽  
pp. 579-584 ◽  
Author(s):  
A. Thangamani ◽  
T. Hofmann
Keyword(s):  
Acridine Orange ◽  
Synthetic Medium ◽  
Acid Protease ◽  
Logarithmic Phase ◽  
Penicillium Janthinellum ◽  
Indirect Connection ◽  
High Enzyme ◽  
Complete Synthetic Medium ◽  
Enzyme Formation

Although peptidase A, the trypsinogen-activating acid protease of Penicillium janthinellum, is formed during the post-logarithmic phase of growth and while the organism is actively sporulating, there is no direct correlation between the production of the enzyme and sporulation. The evidence for this is threefold: (a) in surface cultures acridine orange almost completely suppresses enzyme formation but reduces sporulation to a much smaller extent; (b) 6-ethyl-thiopurine decreases spore formation but stimulates the production of peptidase A; (c) transfer of mycelium in the log phase from a complete, synthetic medium to a medium lacking nitrate induces the formation of high enzyme levels without affecting the spore count.The possibility of an indirect connection between sporulation and peptidase production is not ruled out.

The role of polynucleotides in induced enzyme formation. I

1955 ◽  
Vol 57 (2) ◽  
pp. 475-490 ◽  
Author(s):  
John M. Reiner ◽  
Fred Goodman
Keyword(s):  
Enzyme Formation

scholarly journals DANUBE AND THE FACILITATION OF NAVIGATION ON DANUBE

2017 ◽  
Vol 5 ◽  
pp. 29-33
Author(s):  
Ciprian Beniamin Benea ◽  
Adina Secară OniĹŁa
Keyword(s):  
Legal Framework ◽  
Legal Aspects ◽  
The Political ◽  
Indirect Connection ◽  
Iron Gate ◽  
Political Events ◽  
Gate System ◽  
Ancient Times ◽  
Technical Solutions

With 2857 km in length, the quiet Danube quietly tells Europe’s history. We only must be aware of its story. Since ancient times it was connected with empires, expansion, and navigation. The Romans fully understood its role, and proceeded accordingly. They made it their border, but used it for transporting goods and military, too. After the Dark Ages, all European affairs have been in one way or another connected and influenced by the Danube. Romania’s modern history was influenced by the evolution of international problems connected to this river. The Moldavia and Wallachia 1859’s unification in a single state – Romania – had lot to do with the Danube and it was involved in London’s interests in the Oriental Question. The paper presents shortly the way the legal framework regarding the Danube was developed, and what was Romania’s role in facilitating navigation on the Danube. The main data which inspired this work – regarding both the political-legal aspects, and the technical solutions used to facilitate navigation on Danube – are based on earlier writings and studies of Romanian thinkers such as Antipa, Baicoianu, Dascovici and Gogeanu. The evolution of these aspects has a direct or an indirect connection with the evolution of political events and the economic development in all European states, but their importance is crucial especially for those countries which are located in the Danube’s basin. The main text regarding the political aspects related to the Danube is the Belgrade Convention, which has been the general framework under which riparian countries come together to collaborate and to solve the technical impediments for navigation, such as those imposed by the building of the Iron Gate System. At the same time, this paper signals the role of education in understanding the Danube’s role for riparian countries, and for their possible evolution in connection with this river.

scholarly journals Determinants of Proactive Work Behavior of Employees During the COVID-19 Crisis

2021 ◽  
Author(s):  
Naseer Abbas Khan
Keyword(s):  
Work Behavior ◽  
Toxic Leadership ◽  
Negative Effects ◽  
It Industry ◽  
Indirect Connection ◽  
Mediating Role ◽  
Routine Tasks ◽  
Indirect Association ◽  
Harmonic Interaction

Abstract. As a result of the COVID-19 pandemic, organizations have been forced to use social media and digital applications to carry out routine tasks. This posed several complications and challenges that hindered harmonic interaction between managers and their subordinates. This study investigates the indirect association between toxic leadership and proactive work behavior via psychological safety (PYS). We also investigate the role of perceived insider status (PIS) as a moderator in the association between toxic leadership and PYS as well as the indirect connection between toxic leadership and proactive work behavior. The work is based on the timelag results of 282 supervisor-employee dyads from different small and mid-sized information technology (IT) firms. The results show that toxic leadership has a significant impact on employee PSY and proactive work behavior as well as revealing that PIS substantially mitigated the negative effects of toxic leadership on employee PYS. Similarly, PIS influences the mediating role of PYS in the association between toxic leadership and proactive work behavior of employees. This research is helpful for academics and practitioners involved in human-resource practices in the IT industry.

scholarly journals The Possible Role of Density Current Dynamics in the Generation of Low-Level Vertical Vorticity in Supercells

2017 ◽  
Vol 74 (10) ◽  
pp. 3191-3208 ◽  
Author(s):  
Adam L. Houston
Keyword(s):  
Vertical Motion ◽  
Vertical Shear ◽  
Density Current ◽  
Vertical Vorticity ◽  
Low Level ◽  
Indirect Connection ◽  
Strong Surface ◽  
Boundary Deformation ◽  
Gust Front

Abstract A physical mechanism based on density current dynamics is proposed to explain the generation of low-level vertical vorticity in supercells. This mechanism may serve as one explanation for the associative relationship between environmental low-level vertical shear and the occurrence of significant tornadoes. The mechanism proposed herein represents an indirect connection to the generation of strong surface-based rotation: the barotropic horizontal vorticity associated with the vertical shear acts to amplify existing rotation but does not directly contribute to surface rotation. The proposed mechanism couples the likelihood of a tornado to the vertical shear through the pattern of vertical motion induced through interaction of a deformed gust front and the environmental vertical shear. Results from the experiments conducted to test the veracity of the proposed mechanism illustrate that inferred patterns of tilting and vortex line orientation are consistent with the generation of positive vertical vorticity near the axis of the existing mesocyclone and negative vertical vorticity along the rear-flank gust front. Moreover, inferred tilting is found to scale with the magnitude of the environmental vertical shear, consistent with the climatologies that motivate this work. Experiments also reveal that the proposed mechanism is capable of relating boundary deformation, mesocyclone strength, and hodograph shape to the ultimate likelihood of tornadogenesis.

scholarly journals PHAGE FORMATION IN STAPHYLOCOCCUS MUSCAE CULTURES

1949 ◽  
Vol 32 (3) ◽  
pp. 301-311 ◽  
Author(s):  
Winston H. Price
Keyword(s):  
Active Substance ◽  
Synthetic Medium ◽  
Calf Thymus ◽  
Enzyme Formation ◽  
In Cells

1. A non-dialyzable fraction from fresh bakers' yeast stimulates the formation of S. muscae virus in cells in synthetic medium in the log phase of multiplication. 2. A similar fraction was not found in calf thymus, pancreas, or liver. 3. The active substance in this fraction has been partially purified. 4. This substance is taken up by the cells. In the absence of virus the added substance is metabolized to a form no longer available for virus formation. 5. A purified yeast fraction, which stimulates adaptive enzyme formation in yeast, has been found to stimulate virus formation in the S. muscae system. 6. The similarities between the yeast fraction that stimulates adaptive enzyme formation and the yeast fraction that stimulates virus formation are discussed.

The role of barbituric acid in the nutrition of Bacillus popilliae

1970 ◽  
Vol 16 (9) ◽  
pp. 801-807 ◽  
Author(s):  
Wilson H. Coulter ◽  
Ralph N. Costilow
Keyword(s):  
Barbituric Acid ◽  
Deoxyribonucleic Acid ◽  
Synthetic Medium ◽  
Maximum Growth ◽  
General Distribution ◽  
Significant Stimulation ◽  
Cell Components ◽  
Bacillus Popilliae ◽  
And Control

Barbituric acid is required at a level of 0.1% for maximum growth of Bacillus popilliae in a synthetic medium. However, only a trace amount of that added (~2%) disappeared from the medium during growth. A general distribution of the 14C from 2-14C-barbituric acid among cell components was observed, but a small amount of the radioactivity from this isotope was consistently associated with both ribonucleic acid (RNA) and deoxyribonucleic acid (DNA). The 14C found in RNA was uniformly distributed throughout the 4 S, 16 S, and 23 S RNA fractions, and control experiments indicated that the association of 14C from 2-14C-barbituric acid with the nucleic acids may occur by nonspecific absorption. The presence of barbituric acid resulted in very significant stimulation of both nucleic acid and protein synthesis. The mechanism of this stimulation is unresolved.

scholarly journals Interference with the endosomal acidification by a monoclonal antibody directed toward the 116 (100)-kD subunit of the vacuolar type proton pump.

1994 ◽  
Vol 127 (1) ◽  
pp. 39-53 ◽  
Author(s):  
S B Sato ◽  
S Toyama
Keyword(s):  
Monoclonal Antibody ◽  
Acridine Orange ◽  
Proton Pump ◽  
Viral Fusion ◽  
Enveloped Viruses ◽  
Human Osteosarcoma Cells ◽  
Endosomal Acidification ◽  
Membrane Compartments ◽  
Endosomal Pathway

A monoclonal antibody (OSW2) was prepared by using human osteosarcoma cells. OSW2 was found to be directed toward the 116 (also called 100)- kD protein that uniquely associates to the vacuolar-type proton pump. The antibody specifically localized acidic membrane compartments that could be visualized with acridine orange in many types of human cells. It also reacted with the surface and was internalized along the endosomal pathway. Monitoring the endosome pH by using FITC-dextran and acridine orange suggested that the antibody interfered with low pH. Cell-free experiments indicated that the ATP-dependent acidification was inhibited in endosomes associated with OSW2. In contrast, the antibody gave little effect on the ATPase activity of the solubilized H+ pump. The internalization of OSW2 reduced infectivity of certain enveloped viruses (influenza, SFV, VSV) by 50 to 80%. Inhibition of viral fusion was directly demonstrated by monitoring the fate of octadecylrhodamine-labeled influenza virus fluorescence. These results indicate that the 116 (100)-kD protein is necessary for the control of pH. The antibody represents a novel probe for understanding the role of the endosomal compartments in cellular physiology.

Enrichment of bacteriorhodopsin with isotopically labeled amino acids by biosynthetic incorporation in Halobacterium halobium

1992 ◽  
Vol 38 (11) ◽  
pp. 1181-1185 ◽  
Author(s):  
S. L. Helgerson ◽  
S. L. Siemsen ◽  
E. A. Dratz
Keyword(s):  
Amino Acids ◽  
Synthetic Medium ◽  
Optimal Growth ◽  
Isotopic Labeling ◽  
Maximum Growth ◽  
Integral Membrane Protein ◽  
Maximum Growth Rate ◽  
Halobacterium Halobium ◽  
Isoleucine Leucine ◽  
Complete Synthetic Medium

The growth of Halobacterium halobium was optimized in a chemically defined synthetic medium. Arginine, isoleucine, leucine, lysine, methionine, tyrosine, and valine were found to be essential for growth. Optimal growth rates and cell yields were obtained when the medium was also supplemented with the nonessential amino acids alanine, asparagine, glutamic acid, glycine, phenylalanine, proline, serine, and threonine. The complete synthetic medium supported the same maximum growth rate, cell yield, and production of the integral membrane protein bacteriorhodopsin as was obtained in a complex peptone-based growth medium. Using this defined synthetic medium, isotopically labeled bacteriorhodopsin was produced with several 13C-enriched amino acids. The yield of 13C-labeled bacteriorhodopsin was greater than 35 milligrams of purified protein per litre of cell culture. Key words: bacteriorhodopsin, biosynthetic isotopic labeling, synthetic culture medium, nuclear magnetic resonance.

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