Acridine-bearing PNA for Efficient Protection of Designated Site of DNA from Nuclease S1

2003 ◽  
Vol 32 (8) ◽  
pp. 714-715
Author(s):  
Binzhi Ren ◽  
Sheng Ye ◽  
Xingguo Liang ◽  
Makoto Komiyama
Keyword(s):  
Nuclease S1 ◽  
Efficient Protection

Efficient protection of potato from diseases of various etiology under conditions of the Mosraw region

2019 ◽  
Vol 326 (3) ◽  
pp. 49-53
Author(s):  
M.A. Kuznetsova ◽  
◽  
A.N. Rogozhin ◽  
V.N. Demidova ◽  
T.I. Smetanina ◽  
...  
Keyword(s):  
Efficient Protection

scholarly journals Immune Responses Elicited by Live Attenuated Influenza Vaccines as Correlates of Universal Protection against Influenza Viruses

Vaccines ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 353
Author(s):  
Yo Han Jang ◽  
Baik L. Seong
Keyword(s):  
Immune Responses ◽  
Neutralizing Antibodies ◽  
Influenza Virus Infection ◽  
Influenza Viruses ◽  
Influenza Vaccines ◽  
Immune Correlates ◽  
Protection Efficacy ◽  
Public Health Challenge ◽  
Efficient Protection ◽  
Universal Influenza Vaccine

Influenza virus infection remains a major public health challenge, causing significant morbidity and mortality by annual epidemics and intermittent pandemics. Although current seasonal influenza vaccines provide efficient protection, antigenic changes of the viruses often significantly compromise the protection efficacy of vaccines, rendering most populations vulnerable to the viral infection. Considerable efforts have been made to develop a universal influenza vaccine (UIV) able to confer long-lasting and broad protection. Recent studies have characterized multiple immune correlates required for providing broad protection against influenza viruses, including neutralizing antibodies, non-neutralizing antibodies, antibody effector functions, T cell responses, and mucosal immunity. To induce broadly protective immune responses by vaccination, various strategies using live attenuated influenza vaccines (LAIVs) and novel vaccine platforms are under investigation. Despite superior cross-protection ability, very little attention has been paid to LAIVs for the development of UIV. This review focuses on immune responses induced by LAIVs, with special emphasis placed on the breadth and the potency of individual immune correlates. The promising prospect of LAIVs to serve as an attractive and reliable vaccine platforms for a UIV is also discussed. Several important issues that should be addressed with respect to the use of LAIVs as UIV are also reviewed.

scholarly journals A Novel and Efficient High-Yield Method for Preparing Bacterial Ghosts

Toxins ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 420
Author(s):  
Yi Ma ◽  
Liu Cui ◽  
Meng Wang ◽  
Qiuli Sun ◽  
Kaisheng Liu ◽  
...  
Keyword(s):  
Large Scale ◽  
Expression System ◽  
High Yield ◽  
Wild Type ◽  
High Quality ◽  
Efficient Protection ◽  
Bacterial Ghosts ◽  
Cell Envelopes ◽  
Extracellular Structures ◽  
First Time

Bacterial ghosts (BGs) are empty cell envelopes possessing native extracellular structures without a cytoplasm and genetic materials. BGs are proposed to have significant prospects in biomedical research as vaccines or delivery carriers. The applications of BGs are often limited by inefficient bacterial lysis and a low yield. To solve these problems, we compared the lysis efficiency of the wild-type protein E (EW) from phage ΦX174 and the screened mutant protein E (EM) in the Escherichia coli BL21(DE3) strain. The results show that the lysis efficiency mediated by protein EM was improved. The implementation of the pLysS plasmid allowed nearly 100% lysis efficiency, with a high initial cell density as high as OD600 = 2.0, which was higher compared to the commonly used BG preparation method. The results of Western blot analysis and immunofluorescence indicate that the expression level of protein EM was significantly higher than that of the non-pLysS plasmid. High-quality BGs were observed by SEM and TEM. To verify the applicability of this method in other bacteria, the T7 RNA polymerase expression system was successfully constructed in Salmonella enterica (S. Enterica, SE). A pET vector containing EM and pLysS were introduced to obtain high-quality SE ghosts which could provide efficient protection for humans and animals. This paper describes a novel and commonly used method to produce high-quality BGs on a large scale for the first time.

A Shared Path Protection Mechanism Based on Delay Constraint in the Optical Network

2013 ◽  
Vol 427-429 ◽  
pp. 2237-2244
Author(s):  
Jie Li ◽  
Xing Wei Wang ◽  
Min Huang
Keyword(s):  
Real Time ◽  
Optical Network ◽  
Propagation Delay ◽  
Wavelength Assignment ◽  
Delay Constraint ◽  
Protection Mechanism ◽  
Path Protection ◽  
Important Concern ◽  
Efficient Protection ◽  
Shared Path Protection

Survivability is an important concern in the optical network. In order to offer an effective and efficient protection mechanism that meeting both delay constraint and availability guarantees for real-time services in the optical network, a shared path protection mechanism based on delay constraint is proposed in this paper. Thinking of the processing delay and the propagation delay as main factors which have great effect on the delay of real-time services, the mechanism designs the routing and wavelength assignment schemes for the working path and the protection path. Simulation results show that the proposed mechanism is both feasible and effective.

An Efficient Protection Technique for Last Level STT-RAM Caches in Multi-Core Processors

2017 ◽  
Vol 28 (6) ◽  
pp. 1564-1577 ◽  
Author(s):  
Zahra Azad ◽  
Hamed Farbeh ◽  
Amir Mahdi Hosseini Monazzah ◽  
Seyed Ghassem Miremadi
Keyword(s):  
Efficient Protection
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