scholarly journals Inventory of High-Abundance mRNAs in Skeletal Muscle of Normal Men

1999 ◽  
Vol 9 (5) ◽  
pp. 506-513 ◽  
Author(s):  
Stephen Welle ◽  
Kirti Bhatt ◽  
Charles A. Thornton
Keyword(s):  
Gene Expression ◽  
Ribosomal Proteins ◽  
Vastus Lateralis ◽  
Vastus Lateralis Muscle ◽  
Mrna Species ◽  
Rna Transcripts ◽  
Polyadenylated Rna ◽  
Mitochondrial Transcripts ◽  
Normal Men ◽  
Almost All

The serial analysis of gene expression (SAGE) method was used to generate a catalog of 53,875 short (14 base) expressed sequence tags from polyadenylated RNA obtained from vastus lateralis muscle of healthy young men. Over 12,000 unique tags were detected. The frequency of occurrence of each tag reflects the relative abundance of the corresponding mRNA. The mRNA species that were detected 10 or more times, each comprising ≥0.02% of the mRNA population, accounted for 64% of the mRNA mass but <10% of the total number of mRNA species detected. Almost all of the abundant tags matched mRNA or EST sequences cataloged in GenBank. Mitochondrial transcripts accounted for ∼20% of the polyadenylated RNA. Transcripts encoding proteins of the myofibrils were the most abundant nuclear-encoded mRNAs. Transcripts encoding ribosomal proteins, and those encoding proteins involved in energy metabolism, also were very abundant. The database can be used as a reference for investigations of alterations in gene expression associated with conditions that influence muscle function, such as muscular dystrophies, aging, and exercise.

Assessment of cancer prevention effect of exercise

2021 ◽  
pp. 1-6
Author(s):  
Reza Vafaee ◽  
Mostafa Rezaei Tavirani ◽  
Sina Rezaei Tavirani ◽  
Mohammadreza Razzaghi
Keyword(s):  
Gene Expression ◽  
Cancer Prevention ◽  
Human Health ◽  
Expression Profiles ◽  
Vastus Lateralis ◽  
Gene Expression Profiles ◽  
Gene Expression Omnibus ◽  
Vastus Lateralis Muscle ◽  
Before And After ◽  
Exercise Training Intervention

There are many documents about benefits of exercise on human health. However, evidences indicate to positive effect of exercise on disease prevention, understanding of many aspects of this mechanism need more investigations. Determination of critical genes which effect human health. GSE156249 including 12 gene expression profiles of healthy individual biopsy from vastus lateralis muscle before and after 12-week combined exercise training intervention were extracted from gene expression omnibus (GEO) database. The significant DEGs were included in interactome unit by Cytoscape software and STRING database. The network was analyzed to find the central nodes subnetwork clusters. The nodes of prominent cluster were assessed via gene ontology by using ClueGO. Number of 8 significant DEGs and 100 first neighbors analyzed via network analysis. The network includes 2 clusters and COL3A1, BGN, and LOX were determined as central DEGs. The critical DEGs were involved in cancer prevention process.

Depressed Na+-K+-ATPase activity in skeletal muscle at fatigue is correlated with increased Na+-K+-ATPase mRNA expression following intense exercise

2005 ◽  
Vol 289 (1) ◽  
pp. R266-R274 ◽  
Author(s):  
A. C. Petersen ◽  
K. T. Murphy ◽  
R. J. Snow ◽  
J. A. Leppik ◽  
R. J. Aughey ◽  
...  
Keyword(s):  
Gene Expression ◽  
Atpase Activity ◽  
Mrna Expression ◽  
Binding Sites ◽  
Time Course ◽  
Acute Exercise ◽  
Vastus Lateralis ◽  
Vastus Lateralis Muscle ◽  
Ouabain Binding ◽  
Atpase Gene

We investigated whether depressed muscle Na+-K+-ATPase activity with exercise reflected a loss of Na+-K+-ATPase units, the time course of its recovery postexercise, and whether this depressed activity was related to increased Na+-K+-ATPase isoform gene expression. Fifteen subjects performed fatiguing, knee extensor exercise at ∼40% maximal work output per contraction. A vastus lateralis muscle biopsy was taken at rest, fatigue, 3 h, and 24 h postexercise and analyzed for maximal Na+-K+-ATPase activity via 3- O-methylfluorescein phosphatase (3- O-MFPase) activity, Na+-K+-ATPase content via [3H]ouabain binding sites, and Na+-K+-ATPase α1-, α2-, α3-, β1-, β2- and β3-isoform mRNA expression by real-time RT-PCR. Exercise [352 (SD 267) s] did not affect [3H]ouabain binding sites but decreased 3- O-MFPase activity by 10.7 (SD 8)% ( P < 0.05), which had recovered by 3 h postexercise, without further change at 24 h. Exercise elevated α1-isoform mRNA by 1.5-fold at fatigue ( P < 0.05). This increase was inversely correlated with the percent change in 3- O-MFPase activity from rest to fatigue (%Δ3- O-MFPaserest-fatigue) ( r = −0.60, P < 0.05). The average postexercise (fatigue, 3 h, 24 h) α1-isoform mRNA was increased 1.4-fold ( P < 0.05) and approached a significant inverse correlation with %Δ3- O-MFPaserest-fatigue ( r = −0.56, P = 0.08). Exercise elevated α2-isoform mRNA at fatigue 2.5-fold ( P < 0.05), which was inversely correlated with %Δ3- O-MFPaserest-fatigue ( r = −0.60, P = 0.05). The average postexercise α2-isoform mRNA was increased 2.2-fold ( P < 0.05) and was inversely correlated with the %Δ3- O-MFPaserest-fatigue ( r = −0.68, P < 0.05). Nonsignificant correlations were found between %Δ3- O-MFPaserest-fatigue and other isoforms. Thus acute exercise transiently decreased Na+-K+-ATPase activity, which was correlated with increased Na+-K+-ATPase gene expression. This suggests a possible signal-transduction role for depressed muscle Na+-K+-ATPase activity with exercise.

Carbohydrate ingestion influences skeletal muscle cytokine mRNA and plasma cytokine levels after a 3-h run

2003 ◽  
Vol 94 (5) ◽  
pp. 1917-1925 ◽  
Author(s):  
D. C. Nieman ◽  
J. M. Davis ◽  
D. A. Henson ◽  
J. Walberg-Rankin ◽  
M. Shute ◽  
...  
Keyword(s):  
Gene Expression ◽  
Muscle Glycogen ◽  
Glycogen Content ◽  
Vastus Lateralis ◽  
Maximal Oxygen Consumption ◽  
Vastus Lateralis Muscle ◽  
Cytokine Mrna ◽  
Carbohydrate Ingestion ◽  
Muscle Glycogen Content ◽  
Tnf Α

Sixteen experienced marathoners ran on treadmills for 3 h at ∼70% maximal oxygen consumption (V˙o 2 max) on two occasions while receiving 1 l/h carbohydrate (CHO) or placebo (Pla) beverages. Blood and vastus lateralis muscle biopsy samples were collected before and after exercise. Plasma was analyzed for IL-6, IL-10, IL-1 receptor agonist (IL-1ra), IL-8, cortisol, glucose, and insulin. Muscle was analyzed for glycogen content and relative gene expression of 13 cytokines by using real-time quantitative RT-PCR. Plasma glucose and insulin were higher, and cortisol, IL-6, IL-10, and IL-1ra, but not IL-8, were significantly lower postexercise in CHO vs. Pla. Change in muscle glycogen content did not differ between CHO and Pla ( P = 0.246). Muscle cytokine mRNA content was detected preexercise for seven cytokines in this order (highest to lowest): IL-15, TNF-α, IL-8, IL-1β, IL-12p35, IL-6, and IFN-γ. After subjects ran for 3 h, gene expression above prerun levels was measured for five of these cytokines: IL-1β, IL-6, and IL-8 (large increases), and IL-10 and TNF-α (small increases). The increase in mRNA (fold difference from preexercise) was attenuated in CHO (15.9-fold) compared with Pla (35.2-fold) for IL-6 ( P = 0.071) and IL-8 (CHO, 7.8-fold; Pla, 23.3-fold; P = 0.063). CHO compared with Pla beverage ingestion attenuates the increase in plasma IL-6, IL-10, and IL-1ra and gene expression for IL-6 and IL-8 in athletes running 3 h at 70%V˙o 2 max despite no differences in muscle glycogen content.

Exercise training increases lipid metabolism gene expression in human skeletal muscle

2002 ◽  
Vol 283 (1) ◽  
pp. E66-E72 ◽  
Author(s):  
Rebecca J. Tunstall ◽  
Kate A. Mehan ◽  
Glenn D. Wadley ◽  
Gregory R. Collier ◽  
Arend Bonen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Plasma Membrane ◽  
Exercise Training ◽  
Lipid Oxidation ◽  
Binding Protein ◽  
Regulatory Element ◽  
Vastus Lateralis ◽  
Vastus Lateralis Muscle ◽  
Peroxisome Proliferator ◽  
Cpt I

The effects of a single bout of exercise and exercise training on the expression of genes necessary for the transport and β-oxidation of fatty acids (FA), together with the gene expression of transcription factors implicated in the regulation of FA homeostasis were investigated. Seven human subjects (3 male, 4 female, 28.9 ± 3.1 yr of age, range 20–42 yr, body mass index 22.6 kg/m2, range 17–26 kg/m2) underwent a 9-day exercise training program of 60 min cycling per day at 63% peak oxygen uptake (V˙o 2 peak; 104 ± 14 W). On days 1 and 9 of the program, muscle biopsies were sampled from the vastus lateralis muscle at rest, at the completion of exercise, and again 3 h postexercise. Gene expression of key components of FA transport [FA translocase (FAT/CD36), plasma membrane-associated FA-binding protein], β-oxidation [carntine palmitoyltransferase(CPT) I, β-hydroxyacyl-CoA dehydrogenase] and transcriptional control [peroxisome proliferator-activated receptor (PPAR)α, PPARγ, PPARγ coactivator 1, sterol regulatory element-binding protein-1c] were unaltered by exercise when measured at the completion and at 3 h postexercise. Training increased total lipid oxidation by 24% ( P < 0.05) for the 1-h cycling bout. This increased capacity for lipid oxidation was accompanied by an increased expression of FAT/CD36 and CPT I mRNA. Similarly, FAT/CD36 protein abundance was also upregulated by exercise training. We conclude that enhanced fat oxidation after exercise training is most closely associated with the genes involved in regulating FA uptake across the plasma membrane (FAT/CD36) and across the mitochondrial membrane (CPT I).

Endurance training-induced changes in insulin sensitivity and gene expression

2005 ◽  
Vol 288 (6) ◽  
pp. E1168-E1178 ◽  
Author(s):  
Margarita Teran-Garcia ◽  
Tuomo Rankinen ◽  
Robert A. Koza ◽  
D. C. Rao ◽  
Claude Bouchard
Keyword(s):  
Gene Expression ◽  
Insulin Sensitivity ◽  
Glucose Tolerance ◽  
Candidate Genes ◽  
Molecular Mechanisms ◽  
Vastus Lateralis ◽  
Intravenous Glucose Tolerance Test ◽  
Vastus Lateralis Muscle ◽  
Intravenous Glucose ◽  
Before And After

The beneficial effects of regular physical activity on insulin sensitivity (SI) and glucose tolerance are well documented, with considerable heterogeneity in responsiveness to exercise training (ET). To find novel candidate genes for ET-induced improvement in SI, we used microarray technology. Total RNA was isolated from vastus lateralis muscle before and after 20 wk of exercise from individuals participating in the HERITAGE Family Study. SI index was derived from a frequently sampled intravenous glucose tolerance test using MINMOD Millennium software. Sixteen subjects were selected: eight showing no changes in SI (low responders, LSIR) and eight displaying marked improvement in SI (high responders, HSIR) with ET. The SI increase was about four times greater in HSIR compared with LSIR (+3.6 ± 0.5 vs. −1.2 ± 0.5 μU·ml−1·min−1, mean ± SE), whereas age, body mass index, percent body fat, and baseline SI were similar between the groups. Triplicate microarrays were performed, comparing pooled RNA with HSIR and LSIR individuals for differences in gene expression before and after ET using in situ-generated microarrays (18, 861 genes). Array data were validated by quantitative RT-PCR. Almost twice as many genes showed at least twofold differences between HSIR and LSIR after training compared with pretraining. We identified differentially expressed genes involved in energy metabolism and signaling, novel structural genes, and transcripts of unknown function. Genes of interest upregulated in HSIR include V-Ski oncogene, four-and-a-half LIM domain 1, and titin. Further study of these novel candidate genes should provide a better understanding of molecular mechanisms involved in the improvement in insulin sensitivity in response to regular exercise.

Similar changes of gene expression in human skeletal muscle after resistance exercise and multiple fine needle biopsies

2012 ◽  
Vol 112 (2) ◽  
pp. 289-295 ◽  
Author(s):  
Birgit Friedmann-Bette ◽  
Fides Regina Schwartz ◽  
Holger Eckhardt ◽  
Rudolf Billeter ◽  
Gabriel Bonaterra ◽  
...  
Keyword(s):  
Gene Expression ◽  
Resistance Exercise ◽  
Glucose Transporter ◽  
Vastus Lateralis ◽  
Whole Body ◽  
Control Group ◽  
Marker Genes ◽  
Vastus Lateralis Muscle ◽  
Fine Needle ◽  
Lateralis Muscle

Repeated biopsy sampling from one muscle is necessary to investigate muscular adaptation to different forms of exercise as adaptation is thought to be the result of cumulative effects of transient changes in gene expression in response to single exercise bouts. In a crossover study, we obtained four fine needle biopsies from one vastus lateralis muscle of 11 male subjects (25.9 ± 3.8 yr, 179.2 ± 4.8 cm, 76.5 ± 7.0 kg), taken before (baseline), 1, 4, and 24 h after one bout of squatting exercise performed as conventional squatting or as whole body vibration exercise. To investigate if the repeated biopsy sampling has a confounding effect on the observed changes in gene expression, four fine needle biopsies from one vastus lateralis muscle were also taken from 8 male nonexercising control subjects (24.5 ± 3.7 yr, 180.6 ± 1.2 cm, 81.2 ± 1.6 kg) at the equivalent time points. Using RT-PCR, we observed similar patterns of change in the squatting as well as in the control group for the mRNAs of interleukin 6 (IL-6), IL-6 receptor, insulin-like growth factor 1, p21, phosphofructokinase, and glucose transporter in relation to the baseline biopsy. In conclusion, multiple fine needle biopsies obtained from the same muscle region can per se influence the expression of marker genes induced by an acute bout of resistance exercise.

High-abundance mRNAs in human muscle: comparison between young and old

2000 ◽  
Vol 89 (1) ◽  
pp. 297-304 ◽  
Author(s):  
Stephen Welle ◽  
Kirti Bhatt ◽  
Charles A. Thornton
Keyword(s):  
Gene Expression ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Vastus Lateralis ◽  
Vastus Lateralis Muscle ◽  
Altered Gene Expression ◽  
Detection Frequency ◽  
Altered Gene ◽  
Different Levels

To gain a better understanding of the potential role of altered gene expression in the diminished muscle function in old age, we performed a broad search for transcripts expressed at quantitatively different levels in younger (21–24 yr) and older (66–77 yr) human vastus lateralis muscle by serial analysis of gene expression (SAGE). Because SAGE was based on RNA pooled from muscle of several different subjects, relative concentrations of selected mRNAs also were determined in individual muscle samples by quantitative RT-PCR. There were 702 SAGE tags detected at least 10 times in one or both mRNA pools, and the detection frequency was different (at P < 0.01) between young and older muscle for 89 of these. The ratio of myosin heavy chain 2a mRNA to myosin heavy chain 1 mRNA was reduced in older muscle. The mRNAs encoding several mitochondrial proteins involved in electron transport (including several subunits of cytochrome- c oxidase and NADH dehydrogenase) and subunits of ATP synthase were ∼30% less abundant in older muscle. Several mRNAs encoding enzymes involved in glucose metabolism also were less abundant in older muscle. Analysis of individual samples revealed that the differences suggested by SAGE were not artifacts of atypical gene expression in one or a few individuals. These data suggest that some of the phenotypic changes in senescent muscle may be related to altered gene transcription.

Influence of carbohydrate ingestion on immune changes after 2 h of intensive resistance training

2004 ◽  
Vol 96 (4) ◽  
pp. 1292-1298 ◽  
Author(s):  
D. C. Nieman ◽  
J. M. Davis ◽  
V. A. Brown ◽  
D. A. Henson ◽  
C. L. Dumke ◽  
...  
Keyword(s):  
Gene Expression ◽  
Glycogen Content ◽  
Weight Training ◽  
Vastus Lateralis ◽  
Vastus Lateralis Muscle ◽  
Carbohydrate Ingestion ◽  
Trained Subjects ◽  
Muscle Gene Expression ◽  
Cell Counts ◽  
Tnf Α

Thirty strength-trained subjects were randomized to carbohydrate (CHO) or placebo (Pla) groups and lifted weights for 2 h (10 exercises, 4 sets each, 10 repetitions, with 2- to 3-min rest intervals). Subjects received 10 ml·kg-1·h-1 CHO (6%) or Pla beverages during the weight training bout. Blood, saliva, and vastus lateralis muscle biopsy samples were collected before and after exercise. Blood cell counts were determined, and plasma was analyzed for IL-6, IL-10, IL-1 receptor antagonist (IL-1ra), IL-8, and cortisol. Muscle was analyzed for glycogen content and relative gene expression of 13 cytokines (IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p35, IL-12p40, IL-15, IFN-γ, TNF-α) by use of real-time quantitative RT-PCR. Significant but modest increases were measured for plasma IL-6, IL-10, IL-1ra, and IL-8, but the pattern of increase did not differ between CHO and Pla groups. The rate of decrease in muscle glycogen content did not differ between CHO and Pla ( P = 0.463). Muscle cytokine mRNA was detected preexercise for IL-1β, IL-6, IL-15, IL-8, and TNF-α, and of these, IL-1β, IL-6, IL-8, and TNF-α were significantly increased after the 2-h weight training bout. The increase in mRNA (fold difference from preexercise) did not differ between CHO and Pla groups. In summary, CHO vs. Pla ingestion did not alter modest increases measured for plasma IL-6, IL-10, IL-1ra, and IL-8, and muscle gene expression for IL-1β, IL-6, IL-8, and TNF-α in strength-trained subjects lifting weights intensively for 2 h.

scholarly journals Heightened muscle inflammation susceptibility may impair regenerative capacity in aging humans

2013 ◽  
Vol 115 (6) ◽  
pp. 937-948 ◽  
Author(s):  
Edward K. Merritt ◽  
Michael J. Stec ◽  
Anna Thalacker-Mercer ◽  
Samuel T. Windham ◽  
James M. Cross ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Cell Signaling ◽  
Vastus Lateralis ◽  
Receptor Expression ◽  
Vastus Lateralis Muscle ◽  
Regenerative Capacity ◽  
Muscle Inflammation ◽  
Tnf Α

The regenerative response of skeletal muscle to mechanically induced damage is impaired with age. Previous work in our laboratory suggests this may result from higher proinflammatory signaling in aging muscle at rest and/or a greater inflammatory response to damage. We, therefore, assessed skeletal muscle proinflammatory signaling at rest and 24 h after unaccustomed, loaded knee extension contractions that induced modest muscle damage (72% increase in serum creatine kinase) in a cohort of 87 adults across three age groups (AGE40, AGE61, and AGE76). Vastus lateralis muscle gene expression and protein cell signaling of the IL-6 and TNF-α pathways were determined by quantitative PCR and immunoblot analysis. For in vitro studies, cell signaling and fusion capacities were compared among primary myoblasts from young (AGE28) and old (AGE64) donors treated with TNF-α. Muscle expression was higher (1.5- to 2.1-fold) in AGE76 and AGE61 relative to AGE40 for several genes involved in IL-6, TNF-α, and TNF-like weak inducer of apoptosis signaling. Indexes of activation for the proinflammatory transcription factors signal transducer and activator of transcription-3 and NF-κB were highest in AGE76. Resistance loading reduced gene expression of IL-6 receptor, muscle RING finger 1, and atrogin-1, and increased TNF-like weak inducer of apoptosis receptor expression. Donor myoblasts from AGE64 showed impaired differentiation and fusion in standard media and greater NF-κB activation in response to TNF-α treatment (compared with AGE28). We show for the first time that human aging is associated with muscle inflammation susceptibility (i.e., higher basal state of proinflammatory signaling) that is present in both tissue and isolated myogenic cells and likely contributes to the impaired regenerative capacity of skeletal muscle in the older population.

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