scholarly journals Requirement of yeast Rad1-Rad10 nuclease for the removal of 3'-blocked termini from DNA strand breaks induced by reactive oxygen species

2004 ◽  
Vol 18 (18) ◽  
pp. 2283-2291 ◽  
Author(s):  
S. N. Guzder
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Dna Strand Breaks ◽  
Oxygen Species ◽  
Strand Breaks ◽  
Dna Strand

scholarly journals Lipocalin produced by myelofibrosis cells affects the fate of both hematopoietic and marrow microenvironmental cells

Blood ◽  
2015 ◽  
Vol 126 (8) ◽  
pp. 972-982 ◽  
Author(s):  
Min Lu ◽  
Lijuan Xia ◽  
Yen-Chun Liu ◽  
Tsivia Hochman ◽  
Laetizia Bizzari ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Dna Strand Breaks ◽  
Oxygen Species ◽  
Strand Breaks ◽  
Cd34 Cells ◽  
Key Points ◽  
Dna Strand

Key Points LCN2 acts to generate reactive oxygen species, leading to increased DNA strand breaks and apoptosis in normal CD34+ cells. LCN2 promotes the generation of osteoblasts but diminishes adipogenesis, resembling the composition of the MF marrow microenvironment.

Reactive oxygen species controllable non-thermal helium plasmas for evaluation of plasmid DNA strand breaks

2012 ◽  
Vol 101 (22) ◽  
pp. 224101 ◽  
Author(s):  
Jae Young Kim ◽  
Dong-Hoon Lee ◽  
John Ballato ◽  
Weiguo Cao ◽  
Sung-O Kim
Keyword(s):  
Reactive Oxygen Species ◽  
Plasmid Dna ◽  
Reactive Oxygen ◽  
Dna Strand Breaks ◽  
Oxygen Species ◽  
Strand Breaks ◽  
Dna Strand

scholarly journals Ginger Oleoresin Alleviated γ-Ray Irradiation-Induced Reactive Oxygen Species via the Nrf2 Protective Response in Human Mesenchymal Stem Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Kaihua Ji ◽  
Lianying Fang ◽  
Hui Zhao ◽  
Qing Li ◽  
Yang Shi ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Reactive Oxygen ◽  
Ros Generation ◽  
Oxygen Species ◽  
Ros Scavenging ◽  
Genes Encoding ◽  
Dna Strand

Unplanned exposure to radiation can cause side effects on high-risk individuals; meanwhile, radiotherapies can also cause injury on normal cells and tissues surrounding the tumor. Besides the direct radiation damage, most of the ionizing radiation- (IR-) induced injuries were caused by generation of reactive oxygen species (ROS). Human mesenchymal stem cells (hMSCs), which possess self-renew and multilineage differentiation capabilities, are a critical population of cells to participate in the regeneration of IR-damaged tissues. Therefore, it is imperative to search effective radioprotectors for hMSCs. This study was to demonstrate whether natural source ginger oleoresin would mitigate IR-induced injuries in human mesenchymal stem cells (hMSCs). We demonstrated that ginger oleoresin could significantly reduce IR-induced cytotoxicity, ROS generation, and DNA strand breaks. In addition, the ROS-scavenging mechanism of ginger oleoresin was also investigated. The results showed that ginger oleoresin could induce the translocation of Nrf2 to cell nucleus and activate the expression of cytoprotective genes encoding for HO-1 and NQO-1. It suggests that ginger oleoresin has a potential role of being an effective antioxidant and radioprotective agent.

scholarly journals Through Its Nonstructural Protein NS1, Parvovirus H-1 Induces Apoptosis via Accumulation of Reactive Oxygen Species

2010 ◽  
Vol 84 (12) ◽  
pp. 5909-5922 ◽  
Author(s):  
Georgi Hristov ◽  
Melanie Krämer ◽  
Junwei Li ◽  
Nazim El-Andaloussi ◽  
Rodrigo Mora ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Nonstructural Protein ◽  
Reactive Oxygen ◽  
Double Strand Breaks ◽  
Oxygen Species ◽  
Ns1 Protein ◽  
Dna Double Strand Breaks ◽  
Caspase 9 ◽  
Strand Breaks ◽  
Cycle Arrest

ABSTRACT The rat parvovirus H-1 (H-1PV) attracts high attention as an anticancer agent, because it is not pathogenic for humans and has oncotropic and oncosuppressive properties. The viral nonstructural NS1 protein is thought to mediate H-1PV cytotoxicity, but its exact contribution to this process remains undefined. In this study, we analyzed the effects of the H-1PV NS1 protein on human cell proliferation and cell viability. We show that NS1 expression is sufficient to induce the accumulation of cells in G2 phase, apoptosis via caspase 9 and 3 activation, and cell lysis. Similarly, cells infected with wild-type H-1PV arrest in G2 phase and undergo apoptosis. Furthermore, we also show that both expression of NS1 and H-1PV infection lead to higher levels of intracellular reactive oxygen species (ROS), associated with DNA double-strand breaks. Antioxidant treatment reduces ROS levels and strongly decreases NS1- and virus-induced DNA damage, cell cycle arrest, and apoptosis, indicating that NS1-induced ROS are important mediators of H-1PV cytotoxicity.

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