scholarly journals DMAT, an inhibitor of protein kinase CK2 induces reactive oxygen species and DNA double strand breaks

2009 ◽  
Vol 21 (6) ◽  
Author(s):  
Montenarh
Keyword(s):  
Reactive Oxygen Species ◽  
Protein Kinase ◽  
Protein Kinase Ck2 ◽  
Reactive Oxygen ◽  
Double Strand Breaks ◽  
Oxygen Species ◽  
Dna Double Strand Breaks ◽  
Strand Breaks ◽  
Kinase Ck2

scholarly journals Through Its Nonstructural Protein NS1, Parvovirus H-1 Induces Apoptosis via Accumulation of Reactive Oxygen Species

2010 ◽  
Vol 84 (12) ◽  
pp. 5909-5922 ◽  
Author(s):  
Georgi Hristov ◽  
Melanie Krämer ◽  
Junwei Li ◽  
Nazim El-Andaloussi ◽  
Rodrigo Mora ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Nonstructural Protein ◽  
Reactive Oxygen ◽  
Double Strand Breaks ◽  
Oxygen Species ◽  
Ns1 Protein ◽  
Dna Double Strand Breaks ◽  
Caspase 9 ◽  
Strand Breaks ◽  
Cycle Arrest

ABSTRACT The rat parvovirus H-1 (H-1PV) attracts high attention as an anticancer agent, because it is not pathogenic for humans and has oncotropic and oncosuppressive properties. The viral nonstructural NS1 protein is thought to mediate H-1PV cytotoxicity, but its exact contribution to this process remains undefined. In this study, we analyzed the effects of the H-1PV NS1 protein on human cell proliferation and cell viability. We show that NS1 expression is sufficient to induce the accumulation of cells in G2 phase, apoptosis via caspase 9 and 3 activation, and cell lysis. Similarly, cells infected with wild-type H-1PV arrest in G2 phase and undergo apoptosis. Furthermore, we also show that both expression of NS1 and H-1PV infection lead to higher levels of intracellular reactive oxygen species (ROS), associated with DNA double-strand breaks. Antioxidant treatment reduces ROS levels and strongly decreases NS1- and virus-induced DNA damage, cell cycle arrest, and apoptosis, indicating that NS1-induced ROS are important mediators of H-1PV cytotoxicity.

scholarly journals Regulation of Reactive Oxygen Species byAtmIs Essential for Proper Response to DNA Double-Strand Breaks in Lymphocytes

2006 ◽  
Vol 178 (1) ◽  
pp. 103-110 ◽  
Author(s):  
Keisuke Ito ◽  
Keiyo Takubo ◽  
Fumio Arai ◽  
Hitoshi Satoh ◽  
Sahoko Matsuoka ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Double Strand Breaks ◽  
Oxygen Species ◽  
Dna Double Strand Breaks ◽  
Strand Breaks ◽  
Proper Response

scholarly journals In Benign Barrett's Epithelial Cells, Acid Exposure Generates Reactive Oxygen Species That Cause DNA Double-Strand Breaks

2009 ◽  
Vol 69 (23) ◽  
pp. 9083-9089 ◽  
Author(s):  
Hui Ying Zhang ◽  
Kathy Hormi-Carver ◽  
Xi Zhang ◽  
Stuart J. Spechler ◽  
Rhonda F. Souza
Keyword(s):  
Reactive Oxygen Species ◽  
Epithelial Cells ◽  
Reactive Oxygen ◽  
Double Strand Breaks ◽  
Acid Exposure ◽  
Oxygen Species ◽  
Dna Double Strand Breaks ◽  
Strand Breaks

scholarly journals RNA sequencing supports distinct reactive oxygen species-mediated pathways of apoptosis by high and low size mass fractions of Bay leaf (Lauris nobilis) in HT-29 cells

2015 ◽  
Vol 6 (8) ◽  
pp. 2507-2524 ◽  
Author(s):  
Annabelle L. Rodd ◽  
Katherine Ververis ◽  
Dheeshana Sayakkarage ◽  
Abdul W. Khan ◽  
Haloom Rafehi ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Rna Sequencing ◽  
Reactive Oxygen ◽  
Double Strand Breaks ◽  
High Mass ◽  
Oxygen Species ◽  
Dna Double Strand Breaks ◽  
Strand Breaks ◽  
Mass Fractions ◽  
Ht 29

DNA double strand breaks mediated by high mass bay leaf fraction in HT-29 cells.

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