scholarly journals ‘Simultaneous tracking of cell motility in liquid and at the solid-liquid interface’

2019 ◽  
Author(s):  
Andrew L. Hook ◽  
James L. Flewellen ◽  
Irwin M. Zaid ◽  
Richard M. Berry ◽  
Jean-Frédéric Dubern ◽  
...  

1.AbstractTo better understand key behaviors of living cells, such as bacterial biofilm formation, they must be observed above surfaces and at the interface between the surface and liquid medium. We have established a methodology for label-free imaging and tracking of individual cells simultaneously at both the solid-liquid interface and within the bulk, utilizing imaging modes of digital holographic microscopy (DHM) in 3D, differential interference contrast (DIC) and total internal reflectance microscopy (TIRM) in 2D as well as analysis protocols using a bespoke software package. We illustrate the power of this method by making detailed single cell measurements ofPseudomonas aeruginosain the first minutes of their interaction with a glass surface, focusing on the role of the flagella stators,motABandmotCD. Using this new method we have determined their relative contributions to bulk and near surface motion for populations of cells at the single cell level.

2020 ◽  
Vol 124 (5) ◽  
pp. 2987-2993
Author(s):  
Chi-Kuang Sun ◽  
Yi-Ting Yao ◽  
Chih-Chiang Shen ◽  
Mu-Han Ho ◽  
Tien-Chang Lu ◽  
...  

2021 ◽  
Author(s):  
Wei Zhang ◽  
Qiang Wu ◽  
Ziqi Zeng ◽  
Chuang Yu ◽  
Shijie Cheng ◽  
...  

A soluble organoselenide compound, phenyl diselenide (PDSe), is employed as a soluble electrolyte additive to enhance the kinetics of sulfurized polyacrylonitrile cathode, in which radical exchange in the solid-liquid interface...


2021 ◽  
Vol 11 (6) ◽  
pp. 2464
Author(s):  
Sha Yang ◽  
Neven Ukrainczyk ◽  
Antonio Caggiano ◽  
Eddie Koenders

Modelling of a mineral dissolution front propagation is of interest in a wide range of scientific and engineering fields. The dissolution of minerals often involves complex physico-chemical processes at the solid–liquid interface (at nano-scale), which at the micro-to-meso-scale can be simplified to the problem of continuously moving boundaries. In this work, we studied the diffusion-controlled congruent dissolution of minerals from a meso-scale phase transition perspective. The dynamic evolution of the solid–liquid interface, during the dissolution process, is numerically simulated by employing the Finite Element Method (FEM) and using the phase–field (PF) approach, the latter implemented in the open-source Multiphysics Object Oriented Simulation Environment (MOOSE). The parameterization of the PF numerical approach is discussed in detail and validated against the experimental results for a congruent dissolution case of NaCl (taken from literature) as well as on analytical models for simple geometries. In addition, the effect of the shape of a dissolving mineral particle was analysed, thus demonstrating that the PF approach is suitable for simulating the mesoscopic morphological evolution of arbitrary geometries. Finally, the comparison of the PF method with experimental results demonstrated the importance of the dissolution rate mechanisms, which can be controlled by the interface reaction rate or by the diffusive transport mechanism.


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