scholarly journals Low-Cost Touchscreen Driven Programmable Dual Syringe Pump for Life Science Applications

2018 ◽  
Author(s):  
Valentina E. Garcia ◽  
Jamin Liu ◽  
Joseph L. DeRisi
Keyword(s):  
Life Science ◽  
Narrow Range ◽  
Low Cost ◽  
Stepper Motor ◽  
Raspberry Pi ◽  
Syringe Pump ◽  
Motor Controller ◽  
Python Scripting ◽  
A Cell ◽  
3D Printed

AbstractSyringe pumps are powerful tools able to automate routine laboratory practices that otherwise consume large amounts of manual labor time. Commercially available syringe pumps are expensive, difficult to customize, and often preset for a narrow range of operations. Here, we show how to build a programmable dual syringe pump (PDSP) that overcomes these limitations. The PDSP is driven by a Raspberry Pi paired with a stepper motor controller to allow maximal customization via Python scripting. The entire setup can be controlled by a touchscreen for use without a keyboard or mouse. Furthermore, the PDSP is structured around 3D printed parts, enabling users to change any component for their specific application. We demonstrate one application of the PDSP by using it to generate whole cell lysates using a cell homogenizer in an automated fashion.Specifications table

scholarly journals Highly specific, multiplexed isothermal pathogen detection with fluorescent aptamer readout

2020 ◽  
Author(s):  
Lauren M. Aufdembrink ◽  
Pavana Khan ◽  
Nathaniel J Gaut ◽  
Katarzyna P. Adamala ◽  
Aaron E. Engelhart
Keyword(s):  
Cell Phone ◽  
Pathogen Detection ◽  
Low Cost ◽  
False Positives ◽  
Raspberry Pi ◽  
Side Reactions ◽  
Highly Active ◽  
A Cell ◽  
3D Printed ◽  
Microplate Reader

AbstractIsothermal, cell-free, synthetic biology-based approaches to pathogen detection leverage the power of tools available in biological systems, such as highly active polymerases compatible with lyophilization, without the complexity inherent to live-cell systems, of which Nucleic Acid Sequence Based Amplification (NASBA) is well known. Despite the reduced complexity associated with cell-free systems, side reactions are a common characteristic of these systems. As a result, these systems often exhibit false positives from reactions lacking an amplicon. Here we show that the inclusion of a DNA duplex lacking a promoter and unassociated with the amplicon, fully suppresses false positives, enabling a suite of fluorescent aptamers to be used as NASBA tags (Apta-NASBA). Apta-NASBA has a 1 pM detection limit and can provide multiplexed, multicolor fluorescent readout. Furthermore, Apta-NASBA can be performed using a variety of equipment, for example a fluorescence microplate reader, a qPCR instrument, or an ultra-low-cost Raspberry Pi-based 3D-printed detection platform employing a cell phone camera module, compatible with field detection.

scholarly journals Low-cost touchscreen driven programmable dual syringe pump for life science applications

HardwareX ◽  
2018 ◽  
Vol 4 ◽  
pp. e00027 ◽  
Author(s):  
Valentina E. Garcia ◽  
Jamin Liu ◽  
Joseph L. DeRisi
Keyword(s):  
Life Science ◽  
Low Cost ◽  
Syringe Pump

Prototyping and Simulated Analysis of Autonomous Swarm-Bots

2018 ◽  
Author(s):  
Maulikkumar Dhameliya ◽  
Sidharth Sher ◽  
Souma Chowdhury
Keyword(s):  
Low Cost ◽  
Raspberry Pi ◽  
System A ◽  
Simulated Environment ◽  
3D Printed ◽  
Modular Platform ◽  
And Performance ◽  
Small Form Factor ◽  
Rate Of Success ◽  
Simulated Analysis

Teams of small (mm-to-cm scale) robots, often known as swarm-bots, can provide unique functionality owing to their small form factor, distributed sensing capabilities, resilience to disruptions and agent-loss, and likely low cost. Such swarm-bots are being increasingly touted to support various indoor surveillance, hazard detection, and search and rescue missions. This paper presents the conceptual design, fabrication, and testing of a new cm-scale wheeled swarm-bot. Simulated investigation of a simple particle-swarm-inspired approach to coordinated path planning for these swarm-bots is also presented. The swarm bot is developed around a modular platform, comprising snap-on (3D printed) structural components, a stepper-motor actuated wheel system, a Raspberry Pi computing node, a wireless radio module, a Lipo battery, and proximity sensors; all components are readily detachable, thereby allowing reconfiguration flexibility. Through three design generations, a stable prototype offering >20cm/s speed and ∼50 min endurance, was developed, assembled and tested. A virtual simulated environment is developed by combining MATLAB-based modules and a V-Rep environment, in order to simulate the coordinated operation of these swarm-bots. A 78% rate of success in completing target (light source) search missions was observed during the numerical experiments, and performance robustness was observed to improve with increasing swarm size.

scholarly journals BioSamplr: An open source, low cost automated sampling system for bioreactors

2020 ◽  
Author(s):  
John P. Efromson ◽  
Shuai Li ◽  
Michael D. Lynch
Keyword(s):  
Open Source ◽  
Low Cost ◽  
Raspberry Pi ◽  
Sampling System ◽  
Sample Interval ◽  
3D Printed ◽  
Bioreactor Experiment ◽  
Log File ◽  
Time Required ◽  
The Cost

AbstractAutosampling from bioreactors reduces error, increases reproducibility and offers improved aseptic handling when compared to manual sampling. Additionally, autosampling greatly decreases the hands-on time required for a bioreactor experiment and enables sampling 24 hrs a day. We have designed, built and tested a low cost, open source, automated bioreactor sampling system, the BioSamplr. The BioSamplr can take up to ten samples from a bioreactor at a desired sample interval and cools them to a desired temperature. The device, assembled from low cost and 3D printed components, is controlled wirelessly by a Raspberry Pi, and records all sampling data to a log file. The cost and accessibility of the BioSamplr make it useful for laboratories without access to more expensive and complex autosampling systems.

scholarly journals OPAM, an Open source, 3D printed Low-cost Micro-Manipulator for Single Cell Manipulation

2021 ◽  
Author(s):  
Jiang Xu ◽  
Zhuowei Du ◽  
Paul Hsi Liu ◽  
Yi Kou ◽  
Lin Chen
Keyword(s):  
Single Cell ◽  
Open Source ◽  
Low Cost ◽  
Cell Manipulation ◽  
Stepper Motor ◽  
Great Promise ◽  
3D Printer ◽  
Cell Capture ◽  
Entry Level ◽  
3D Printed

We introduce OPAM, an Open source, low-cost (under $150), 3D-Printed, stepper motor driven, Arduino based, single cell Micromanipulator (OPAM). Modification of a commercial stepper motor led to dramatically increased stability and maneuverability of the motor, based on which the micromanipulator was designed. All components of this micromanipulator can be 3D printed using an entry-level 3D printer and assembled with ease. With this single cell manipulator, successful targeted single cell capture and transfer was confirmed under the microscope, which showed great promise for single cell related experiments.

scholarly journals Programmable Syringe Pump for Selective Micro Droplet Deposition

2019 ◽  
Vol 19 (2) ◽  
pp. 75
Author(s):  
Erry Dwi Kurniawan ◽  
Alwin Adam ◽  
Muhammad Ichlasul Salik ◽  
Paulus Lobo Gareso
Keyword(s):  
Flow Rate ◽  
Flow Direction ◽  
Low Cost ◽  
Stepper Motor ◽  
Average Error ◽  
Syringe Pump ◽  
Pump System ◽  
Lead Screw ◽  
Mechanical Unit ◽  
Maximum Average Error

Micro/nanopatterns with micro deposition techniques have been used in various applications such as flexible electronic devices, biosensing, and biological tissue engineering. For depositing a small size of droplets that can be controlled, structured and patterned precisely is a very important process for microfabrication. In this study, we developed a low cost and simple system for fabricating micro/nanostructure by a selective micro deposition process using a syringe pump. This method is an additive fabrication method where selective droplet materials are released through a needle of the syringe pump. By translating the rotating stepper motor into a linear movement of the lead screw, it will press the plunger of the syringe and give a force to the fluid inside the syringe, hence a droplet can be injected out. The syringe pump system consists of a syringe, the mechanical unit, and the controller unit. A stepper motor, the lead screw, and the mechanical components are used for the mechanical unit. Arduino Uno microcontroller is used as the controller unit and can be programmed by the computer through GUI (Graphical User Interface). The input parameters, such as the push or pull of flow direction, flow rate, the droplet volume, and syringe size dimension can be inputted by the user as their desired value via keypad or the computer. The measurement results show that the syringe pump has characteristics: the maximum average error value of the measured volume is 2.5% and the maximum average error value of the measured flow rate is 14%. The benefits of a syringe pump for micro deposition can overcome photolithography weaknesses, which require an etching and stencil process in the manufacture of semiconductors. Combining two or more syringes into one system with different droplet materials can be used as a promising method for 3D microfabrication in the future.

scholarly journals PiFlow: A Biocompatible Low-Cost Programmable Dynamic Flow Pumping System Utilizing a Raspberry Pi Zero and Commercial Piezoelectric Pumps

2017 ◽  
Author(s):  
Timothy Kassis ◽  
Paola M. Perez ◽  
Chloe J. W. Yang ◽  
Luis R. Soenksen ◽  
David L. Trumper ◽  
...  
Keyword(s):  
Low Cost ◽  
Raspberry Pi ◽  
Dynamic Flow ◽  
Flow Rates ◽  
Use Of Time ◽  
Pumping System ◽  
Cellular Environment ◽  
Cell Culture Systems ◽  
Peristaltic Pumps ◽  
3D Printed

AbstractWith the rise of research utilizing microphysiological systems (MPSs), the need for tools that enable the physiological mimicking of the relevant cellular environment is vital. The limited ability to reproduce crucial features of the microenvironment, such as surrounding fluid flow and dynamic changes in biochemical stimuli, severely limits the types of experiments that can be carried out. Current equipment to achieve this, such as syringe and peristaltic pumps, is expensive, large, difficult to program and has limited potential for scalability. Here, we present a new pumping platform that is open-source, low-cost, modular, scalable, fully-programmable and easy to assemble that can be incorporated into cell culture systems to better recapitulate physiological environments. By controlling two commercially available piezoelectric pumps using a Raspberry Pi Zero microcontroller, the system is capable of producing arbitrary dynamic flow profiles with reliable flow rates ranging from 1 to 3,000 µL/min as specified by an easily programmable Python-based script. We validated the accuracy of the flow rates, the use of time-varying profiles, and the practicality of the system by creating repeatable dynamic concentration profiles using a 3D-printed static micromixer.

Pioneering Low-Cost 3D-Printed Transtibial Prosthetics to Serve a Rural Population in Sierra Leone

2020 ◽  
Author(s):  
Merel van der Stelt ◽  
Martin P. Grobusch ◽  
Abdul R. Koroma ◽  
Marco Papenburg ◽  
Ismaila Kebbie ◽  
...  
Keyword(s):  
Sierra Leone ◽  
Rural Population ◽  
Low Cost ◽  
3D Printed
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