scholarly journals Nighttime gibberellin biosynthesis is influenced by fluctuating environmental conditions and contributes to growth adjustments of Arabidopsis leaves

2020 ◽  
Author(s):  
Putri Prasetyaningrum ◽  
Lorenzo Mariotti ◽  
Maria Cristina Valeri ◽  
Giacomo Novi ◽  
Stijn Dhondt ◽  
...  
Keyword(s):  
Carbon Source ◽  
Plant Growth ◽  
Environmental Conditions ◽  
Light Availability ◽  
Growth Control ◽  
Leaf Expansion ◽  
Gibberellin Biosynthesis ◽  
Carbon Availability ◽  
Leaf Expansion Rate ◽  
Short Timeframe

AbstractOptimal plant growth performance requires that the action of growth signals, such as gibberellins (GA), are coordinated with the availability of photo-assimilates. Here, we studied the links between gibberellin biosynthesis and carbon availability, and the subsequent effects on growth. The results presented here show that carbon availability, light and dark cues, and the clock ensure the timing and magnitude of gibberellin biosynthesis and that disruption of these mechanisms results in reduced gibberellin levels and expression of downstream genes. Carbon dependent nighttime induction of GIBBERELLIN 3-BETA-DIOXYGENASE 1 (GA3ox1) was severely hampered when preceded by a day of lowered light availability, leading specifically to reduced bioactive GA4 levels, and coinciding with a decline in leaf expansion rate during the night. We attribute this decline in leaf expansion mostly to reduced photo-assimilates. However, plants where gibberellin limitation was alleviated had significantly improved expansion demonstrating the relevance of gibberellins in growth control under varying carbon availability. Carbon dependent expression of upstream gibberellin biosynthesis genes (KAURENE SYNTHASE, KS and GIBBERELLIN 20 OXIDASE 1, GA20ox1) was not translated into metabolite changes within this short timeframe. We propose a model where the extent of nighttime biosynthesis of bioactive GA4 by GA3ox1 is determined by starch, as the nighttime carbon source, and so provides day-to-day adjustment of gibberellin responses.

scholarly journals Biosurfactant from endophytic Bacillus pumilus 2A: physicochemical characterization, production and optimization and potential for plant growth promotion

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Olga Marchut-Mikołajczyk ◽  
Piotr Drożdżyński ◽  
Arkadiusz Polewczyk ◽  
Wojciech Smułek ◽  
Tadeusz Antczak
Keyword(s):  
Phaseolus Vulgaris ◽  
Carbon Source ◽  
Plant Growth ◽  
Taguchi Method ◽  
Beta Vulgaris ◽  
Food Processing ◽  
Bacillus Pumilus ◽  
Brewer’S Spent Grain ◽  
Spent Grain ◽  
By Products

Abstract Background Microbial surfactants called biosurfactants, thanks to their high biodegradability, low toxicity and stability can be used not only in bioremediation and oil processing, but also in the food and cosmetic industries, and even in medicine. However, the high production costs of microbial surfactants and low efficiency limit their large-scale production. This requires optimization of management conditions, including the possibility of using waste as a carbon source, such as food processing by-products. This papers describes the production and characterization of the biosurfactant obtained from the endophytic bacterial strain Bacillus pumilus 2A grown on various by-products of food processing and its potential applications in supporting plant growth. Four different carbon and nitrogen sources, pH, inoculum concentration and temperature were optimized within Taguchi method. Results Optimization of bioprocess within Taguchi method and experimental analysis revealed that the optimal conditions for biosurfactant production were brewer’s spent grain (5% w/v), ammonium nitrate (1% w/v), pH of 6, 5% of inoculum, and temperature at 30 °C, leading to 6.8 g/L of biosurfactant. Based on gas chromatography–mass spectrometry and Fourier transform infrared spectroscopy analysis produced biosurfactant was determined as glycolipid. Obtained biosurfactant has shown high and long term thermostability, surface tension of 47.7 mN/m, oil displacement of 8 cm and the emulsion index of 69.11%. The examined glycolipid, used in a concentration of 0.2% significantly enhanced growth of Phaseolus vulgaris L. (bean), Raphanus L. (radish), Beta vulgaris L. (beetroot). Conclusions The endophytic Bacillus pumilus 2A produce glycolipid biosurfactant with high and long tem thermostability, what makes it useful for many purposes including food processing. The use of brewer’s spent grain as the sole carbon source makes the production of biosurfactants profitable, and from an environmental point of view, it is an environmentally friendly way to remove food processing by products. Glycolipid produced by endophytic Bacillus pumilus 2A significantly improve growth of Phaseolus vulgaris L. (bean), Raphanus L. (radish), Beta vulgaris L. (beetroot). Obtained results provide new insight to the possible use of glycolipids as plant growth promoting agents.

scholarly journals Physiology of Methylotrophs Living in the Phyllosphere

2021 ◽  
Vol 9 (4) ◽  
pp. 809
Author(s):  
Hiroya Yurimoto ◽  
Kosuke Shiraishi ◽  
Yasuyoshi Sakai
Keyword(s):  
Plant Growth ◽  
Crop Yield ◽  
Environmental Conditions ◽  
Sole Source ◽  
Current Understanding ◽  
Methylotrophic Bacteria ◽  
Cellular Mechanisms ◽  
Diurnal Cycles ◽  
Promote Plant Growth ◽  
Increase Crop Yield

Methanol is abundant in the phyllosphere, the surface of the above-ground parts of plants, and its concentration oscillates diurnally. The phyllosphere is one of the major habitats for a group of microorganisms, the so-called methylotrophs, that utilize one-carbon (C1) compounds, such as methanol and methane, as their sole source of carbon and energy. Among phyllospheric microorganisms, methanol-utilizing methylotrophic bacteria, known as pink-pigmented facultative methylotrophs (PPFMs), are the dominant colonizers of the phyllosphere, and some of them have recently been shown to have the ability to promote plant growth and increase crop yield. In addition to PPFMs, methanol-utilizing yeasts can proliferate and survive in the phyllosphere by using unique molecular and cellular mechanisms to adapt to the stressful phyllosphere environment. This review describes our current understanding of the physiology of methylotrophic bacteria and yeasts living in the phyllosphere where they are exposed to diurnal cycles of environmental conditions.

scholarly journals Abiotic Stresses Shift Belowground Populus-Associated Bacteria Toward a Core Stress Microbiome

mSystems ◽  
2018 ◽  
Vol 3 (1) ◽  
Author(s):  
Collin M. Timm ◽  
Kelsey R. Carter ◽  
Alyssa A. Carrell ◽  
Se-Ran Jun ◽  
Sara S. Jawdy ◽  
...  
Keyword(s):  
Plant Growth ◽  
Environmental Conditions ◽  
Bacterial Communities ◽  
Abiotic Stresses ◽  
Growth Conditions ◽  
Plant Host ◽  
Associated Bacteria ◽  
Poplar Trees

The identification of a common “stress microbiome” indicates tightly controlled relationships between the plant host and bacterial associates and a conserved structure in bacterial communities associated with poplar trees under different growth conditions. The ability of the microbiome to buffer the plant from extreme environmental conditions coupled with the conserved stress microbiome observed in this study suggests an opportunity for future efforts aimed at predictably modulating the microbiome to optimize plant growth.

scholarly journals Strip-till technology - a method for uniformity in the emergence and plant growth of winter rapeseed (Brassica napus L.) in different environmental conditions of Northern Poland

2018 ◽  
pp. 194-199 ◽  
Author(s):  
Iwona Jaskulska ◽  
Lech Gałęzewski ◽  
Mariusz Piekarczyk ◽  
Dariusz Jaskulski
Keyword(s):  
Plant Growth ◽  
Environmental Conditions ◽  
Block Design ◽  
Thermal Conditions ◽  
Weather Conditions ◽  
Field Trials ◽  
Initial Growth ◽  
Brassica Napus L ◽  
Initial Development ◽  
Winter Rapeseed

The emergence of plants is especially important for the winter crops that are grown in the challenging environmental conditions of many countries in Central and Eastern Europe. The emergence and initial growth of winter rapeseed were compared in field trials in a randomized block design with three replicates for plants sown in conventional tillage systems (CT) and strip-till (ST), which had different weather conditions and on soil with a non-uniform texture over a period of two years. Sowing in the CT was carried out using Horsch Pronto 4DC (Germany) at a row distance of 0.29 m. The ST operations were performed using a Pro-Til 4T drill manufactured by Mzuri Limited (Great Britain) - row spacing of 0.36 m. In favourable rainfall and thermal conditions, the density of winter rapeseed plants two weeks after sowing was found to be higher if it was sown after the CT than in the ST system. In the year that had a serious shortage of rainfall during the sowing period, a considerably higher density of plants was achieved using the ST system. The uniformity of plant growth using the ST technology in soil with a varied texture, especially in a year with an unfavourable distribution of rainfall, was proven by less variability in the number of leaves in the rosette, in the dry mass of the leaf rosette and in the root neck thickness of the winter rapeseed than in the CT system. The ST system can create good conditions for the initial development and preparation of rapeseed plants for wintering.

Biofertilizers-Mediated Sustainable Plant Growth and Production Under Adverse Environmental Conditions

2021 ◽  
pp. 437-457
Author(s):  
Swetika Porwal ◽  
Akhilesh Kumar Singh ◽  
Ashok Kumar Yadav ◽  
Sudhir Kumar ◽  
Paras Porwal
Keyword(s):  
Plant Growth ◽  
Environmental Conditions ◽  
Adverse Environmental Conditions

Expression and relationships of resistance to white rust (Albugo candida) at cotyledonary, seedling, and flowering stages in Brassica juncea germplasm from Australia, China, and India

2007 ◽  
Vol 58 (3) ◽  
pp. 259 ◽  
Author(s):  
C. X. Li ◽  
K. Sivasithamparam ◽  
G. Walton ◽  
P. Salisbury ◽  
W. Burton ◽  
...  
Keyword(s):  
Plant Growth ◽  
Brassica Juncea ◽  
Environmental Conditions ◽  
Host Resistance ◽  
Growth Stages ◽  
Sources Of Resistance ◽  
Albugo Candida ◽  
White Rust ◽  
China And India ◽  
Canola Quality

White rust (Albugo candida) is a highly destructive disease of oilseed Brassicas such as Brassica juncea and B. rapa. Most commercial B. juncea or B. rapa varieties are highly susceptible and yield losses from combined infection of leaves and inflorescences can be up to 20% or 60% in Australia and India, respectively. In Australia, canola-quality B. juncea has been developed to extend oilseed Brassica production into lower rainfall areas, with the first commercial B. juncea canola-quality variety planned for release in 2006. It is essential to identify useful sources of host resistance in B. juncea as breeding and/or selection of material for resistance is the most cost-effective method of delivering control for farmers. Three experiments were undertaken under controlled-environmental conditions to identify the best methods of characterising host resistance and to identify sources of resistance in B. juncea germplasm from Australia, China, and India. Forty-four B. juncea genotypes, viz. 22 from India, 12 from Australia, and 10 from China, were tested. Four Chinese genotypes (CBJ-001, CBJ-002, CBJ-003, CBJ-004) and one Australian genotype (JR049) consistently showed high resistance to A. candida across the different plant growth stages against a pathotype prevailing in Australia. Similarly, the most susceptible genotypes (viz. Indian genotypes RH781, RL1359, RH819) were extremely susceptible irrespective of the plant growth stage. Overall, although disease severity on cotyledons and leaves at the different growth stages was significantly and positively correlated, there was, however, no significant correlation between the number of stagheads and any of the other disease parameters measured. Our study demonstrates that controlled-environmental conditions are suitable for rapid identification of resistant genotypes and that genotypes with high levels of resistance can be reliably identified at the cotyledonary, seedling, or flowering stages.

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