scholarly journals The hidden complexity of Mendelian traits across yeast natural populations

2016 ◽  
Author(s):  
Jing Hou ◽  
Anastasie Sigwalt ◽  
David Pflieger ◽  
Jackson Peter ◽  
Jacky de Montigny ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Natural Population ◽  
Large Population ◽  
Natural Populations ◽  
Common Variants ◽  
Yeast Saccharomyces Cerevisiae ◽  
Inheritance Patterns ◽  
First Time ◽  
The Continuum

AbstractMendelian traits are considered as the lower end of the complexity spectrum of heritable phenotypes. However, more than a century after the rediscovery of Mendel’s law, the global landscape of monogenic variants as well as their effects and inheritance patterns within natural populations is still not well understood. Using the yeast Saccharomyces cerevisiae, we performed a species-wide survey of Mendelian traits across a large population of isolates. We generated offspring from 41 unique parental pairs, and analyzed 1,105 cross/trait combinations. We found that 8.9% of the cases were Mendelian. Most were caused by common variants showing stable inheritances in a natural population. However, we also found that a rare monogenic variant related to drug resistance displayed a significant and variable expressivity across different genetic backgrounds, leading to modified inheritances ranging from intermediate to high complexities. Our results illustrate for the first time the continuum of the hidden complexity of a monogenic mutation, where genotype is hardly predictive of phenotype.

scholarly journals Smoking is associated with significantly increased green autofluorescence intensity and asymmetry of the skin and the fingernails of natural populations, population high-risk of developing stroke, and population of acute ischemic stroke

2020 ◽  
Author(s):  
Mingchao Zhang ◽  
Yue Tao ◽  
Danhong Wu ◽  
Weihai Ying
Keyword(s):  
Risk Factor ◽  
Ischemic Stroke ◽  
Acute Ischemic Stroke ◽  
Natural Population ◽  
Health Management ◽  
Large Population ◽  
Natural Populations ◽  
Pathological Changes ◽  
Non Invasive ◽  
Rapid Monitoring

AbstractTobacco smoking is an important risk factor for numerous diseases. It is critically needed to search for the biomarkers of smoking for non-invasive and rapid monitoring of the pathological changes of smokers’ body. Our current study has indicated that green autofluorescence (AF) of the fingernails and certain locations of the skin is a novel biomarker for smoking: First, for the natural population at age between 20 - 50 years of old, both the green AF intensity and the AF asymmetry of the Index Fingernails and the skin of Dorsal Index Fingers of the smokers were remarkably higher than those of the non-smokers. Second, for the natural population, the population at risk of developing acute ischemic stroke (AIS) and the AIS population at age between 50 - 80 years of old, both the AF intensity and the AF asymmetry of the Index Fingernails and the skin of Dorsal Index Fingers of the smokers were also remarkably higher than those of the non-smokers. Third, ROC analyses using the green AF intensity of the Index Fingernails showed that the AUC values were 0.796 to 0.889 for differentiating the smokers and the non-smokers in these three populations. Collectively, our study has indicated that increased green AF intensity of the fingernails and certain locations of the skin is a novel biomarker for smoking. Based on this finding, pathological alterations of smokers’ body may be monitored non-invasively and efficiently, which could be highly valuable for the health management of the large population of tobacco smokers.

Amiodarone inhibits multiple drug resistance in yeast Saccharomyces cerevisiae

2009 ◽  
Vol 191 (8) ◽  
pp. 675-679 ◽  
Author(s):  
Dmitry A. Knorre ◽  
Tatiana N. Krivonosova ◽  
Olga V. Markova ◽  
Fedor F. Severin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Multiple Drug Resistance ◽  
Multiple Drug ◽  
Yeast Saccharomyces Cerevisiae

scholarly journals SEA and GATOR 10 Years Later

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2689
Author(s):  
Yahir A. Loissell-Baltazar ◽  
Svetlana Dokudovskaya
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Neurodegenerative Diseases ◽  
Human Homologue ◽  
Yeast Saccharomyces Cerevisiae ◽  
Multiple Functions ◽  
Nutrient Response ◽  
The Past ◽  
Upstream Regulator ◽  
Mtorc1 Pathway ◽  
First Time

The SEA complex was described for the first time in yeast Saccharomyces cerevisiae ten years ago, and its human homologue GATOR complex two years later. During the past decade, many advances on the SEA/GATOR biology in different organisms have been made that allowed its role as an essential upstream regulator of the mTORC1 pathway to be defined. In this review, we describe these advances in relation to the identification of multiple functions of the SEA/GATOR complex in nutrient response and beyond and highlight the consequence of GATOR mutations in cancer and neurodegenerative diseases.

scholarly journals Multiple-drug-resistance phenomenon in the yeast Saccharomyces cerevisiae: involvement of two hexose transporters.

1997 ◽  
Vol 17 (9) ◽  
pp. 5453-5460 ◽  
Author(s):  
A Nourani ◽  
M Wesolowski-Louvel ◽  
T Delaveau ◽  
C Jacq ◽  
A Delahodde
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Multidrug Resistance ◽  
Abc Transporters ◽  
Multiple Drug Resistance ◽  
Kluyveromyces Lactis ◽  
Major Facilitator Superfamily ◽  
Multiple Drug ◽  
Yeast Saccharomyces Cerevisiae ◽  
Hexose Transporters

In the yeast Saccharomyces cerevisiae, multidrug resistance to unrelated chemicals can result from overexpression of ATP-binding cassette (ABC) transporters such as Pdr5p, Snq2p, and Yor1p. Expression of these genes is under the control of two homologous zinc finger-containing transcription regulators, Pdr1p and Pdr3p. Here, we describe the isolation, by an in vivo screen, of two new Pdr1p-Pdr3p target genes: HXT11 and HXT9. HXT11 and HXT9, encoding nearly identical proteins, have a high degree of identity to monosaccharide transporters of the major facilitator superfamily (MFS). In this study, we show that the HXT11 product, which allows glucose uptake in a glucose permease mutant (rag1) strain of Kluyveromyces lactis, is also involved in the pleiotropic drug resistance process. Loss of HXT11 and/or HXT9 confers cycloheximide, sulfomethuron methyl, and 4-NQO (4-nitroquinoline-N-oxide) resistance. Conversely, HXT11 overexpression increases sensitivity to these drugs in the wild-type strain, an effect which is more pronounced in a strain having both PDR1 and PDR3 deleted. These data show that the two putative hexose transporters Hxt11p and Hxt9p are transcriptionally regulated by the transcription factors Pdr1p and Pdr3p, which are known to regulate the production of ABC transporters required for drug resistance in yeast. We thus demonstrate the existence of genetic interactions between genes coding for two classes of transporters (ABC and MFS) to control the multidrug resistance process.

Dodecyltriphenylphosphonium inhibits multiple drug resistance in the yeast Saccharomyces cerevisiae

2014 ◽  
Vol 450 (4) ◽  
pp. 1481-1484 ◽  
Author(s):  
Dmitry A. Knorre ◽  
Olga V. Markova ◽  
Ekaterina A. Smirnova ◽  
Iuliia E. Karavaeva ◽  
Svyatoslav S. Sokolov ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Multiple Drug Resistance ◽  
Multiple Drug ◽  
Yeast Saccharomyces Cerevisiae

Alkaloids of Crinum x powellii “Album” (Amaryllidaceae) and their Topoisomerase Inhibitory Activity

2007 ◽  
Vol 62 (3-4) ◽  
pp. 223-226 ◽  
Author(s):  
Jaime Niño ◽  
Gina M. Hincapié ◽  
Yaned M. Correa ◽  
Oscar M. Mosquera
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Inhibitory Activity ◽  
Topoisomerase I ◽  
Soluble Fraction ◽  
Genetically Engineered ◽  
Yeast Saccharomyces Cerevisiae ◽  
Spectroscopic Analyses ◽  
Nmr Techniques ◽  
First Time

Abstract The alkaloids lycorine, 1-O-acetyllycorine and ismine were isolated from the basic dichloromethane- soluble fraction of Crinum x powellii “Album” bulbs. The alkaloid structures were established by physical and spectroscopic analyses, including 1D NMR techniques and GCMS analysis. The three alkaloids are reported for the first time for this hybrid. Additionally, the three alkaloids isolated were tested against a mechanism-based bioassay utilizing genetically engineered mutants of the yeast Saccharomyces cerevisiae strains RAD+, RAD52Y and RS321 where lycorine was the only alkaloid that displayed moderate topoisomerase I inhibitory activity.

scholarly journals BIOSORBENTS BASED ON POLYSACCHARIDES. EVALUATION OF SORPTION CAPACITY IN RELATION TO URANIUM AND THORIUM

2019 ◽  
pp. 431-440
Author(s):  
Anatoliy Petrovich Karmanov ◽  
Al'bert Vladimirovich Kanarsky ◽  
Lyudmila Sergeyevna Kocheva ◽  
Zosia Al'bertovna Kanarskaya ◽  
Venera Maratovna Gematdinova ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sorption Capacity ◽  
Water Soluble ◽  
Yeast Saccharomyces Cerevisiae ◽  
High Sorption Capacity ◽  
Strong Adsorption ◽  
High Sorption ◽  
Physical Phenomena ◽  
First Time ◽  
The Relationship

Study of sorption of heavy natural radionuclide’s uranium and thorium from water by β-gluсancontaining sorbents obtained from biomass of yeast Saccharomyces Cerevisiae and bran of oat Avena sativa was carried out. It is shown that the content of mobile (water-soluble, exchange and acid-soluble) and fixed forms of uranium on investigated β-glucans vary considerably. It is found that the extent of irreversible sorption of uranium does not exceed 58.6%. For the first time shown that β-glucans have high sorption capacity in ratio of thorium. In the conditions of the experiments it was retrieved more than 99% of thorium from the water. The content of fixed form of thorium reaches 94% of the sorbed. Characteristics of surface and capillary-porous structure of samples were defined. The correlation relationships between rates of adsorption and specific surface of preparations were installed. An analysis of the relationship between sorption capacity and various properties of glucans leads to the conclusion that the most important role for the implementation of a strong adsorption of heavy radionuclides belongs to chemisorptions mechanisms, while the contribution of surface physical phenomena is not essential. It is shown that the highest strong adsorption of thorium is characterized by a sample representing the cell walls of yeast Saccharomyces cerevisiae. The findings suggest of β-glucans prospects in practical terms and their use as polyfunctional enterosorbеnts.

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