scholarly journals SEA and GATOR 10 Years Later

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2689
Author(s):  
Yahir A. Loissell-Baltazar ◽  
Svetlana Dokudovskaya
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Neurodegenerative Diseases ◽  
Human Homologue ◽  
Yeast Saccharomyces Cerevisiae ◽  
Multiple Functions ◽  
Nutrient Response ◽  
The Past ◽  
Upstream Regulator ◽  
Mtorc1 Pathway ◽  
First Time

The SEA complex was described for the first time in yeast Saccharomyces cerevisiae ten years ago, and its human homologue GATOR complex two years later. During the past decade, many advances on the SEA/GATOR biology in different organisms have been made that allowed its role as an essential upstream regulator of the mTORC1 pathway to be defined. In this review, we describe these advances in relation to the identification of multiple functions of the SEA/GATOR complex in nutrient response and beyond and highlight the consequence of GATOR mutations in cancer and neurodegenerative diseases.

scholarly journals Combined Use of Lachancea thermotolerans and Schizosaccharomyces pombe in Winemaking: A Review

2020 ◽  
Vol 8 (5) ◽  
pp. 655 ◽  
Author(s):  
Santiago Benito
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Schizosaccharomyces Pombe ◽  
Oenococcus Oeni ◽  
Quality Parameters ◽  
Ethyl Carbamate ◽  
Wine Quality ◽  
The Past ◽  
Combined Use ◽  
First Time ◽  
Lachancea Thermotolerans

The combined use of Lachancea thermotolerans and Schizosaccharomyces pombe is a new winemaking biotechnology that aims to solve some modern industrial oenology problems related to warm viticulture regions. These areas are characterized for producing musts with high levels of sugar that can potentially be converted into wines with elevated ethanol contents, which are usually associated with high pH levels. This biotechnology was reported for the first time in 2015, and since then, several scientific articles have been published regarding this topic. These reported scientific studies follow an evolution similar to that performed in the past for Saccharomyces cerevisiae and Oenococcus oeni; they start by reporting results for basic winemaking parameters at the beginning, later continuing with more advanced parameters. This review compares the results of different researchers that have applied this new biotechnology and have studied wine quality parameters such as ethanol, glycerol, malic acid, lactic acid, amino acids, aroma compounds, or anthocyanins. It is shown that the new biotechnology is repeatedly reported to solve specific winemaking problems such as the lack of acidity, biogenic amines, ethyl carbamate, or undesirable color losses. Such results highlight this biotechnology as a promising option for warm viticulture areas.

scholarly journals The hidden complexity of Mendelian traits across yeast natural populations

2016 ◽  
Author(s):  
Jing Hou ◽  
Anastasie Sigwalt ◽  
David Pflieger ◽  
Jackson Peter ◽  
Jacky de Montigny ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Natural Population ◽  
Large Population ◽  
Natural Populations ◽  
Common Variants ◽  
Yeast Saccharomyces Cerevisiae ◽  
Inheritance Patterns ◽  
First Time ◽  
The Continuum

AbstractMendelian traits are considered as the lower end of the complexity spectrum of heritable phenotypes. However, more than a century after the rediscovery of Mendel’s law, the global landscape of monogenic variants as well as their effects and inheritance patterns within natural populations is still not well understood. Using the yeast Saccharomyces cerevisiae, we performed a species-wide survey of Mendelian traits across a large population of isolates. We generated offspring from 41 unique parental pairs, and analyzed 1,105 cross/trait combinations. We found that 8.9% of the cases were Mendelian. Most were caused by common variants showing stable inheritances in a natural population. However, we also found that a rare monogenic variant related to drug resistance displayed a significant and variable expressivity across different genetic backgrounds, leading to modified inheritances ranging from intermediate to high complexities. Our results illustrate for the first time the continuum of the hidden complexity of a monogenic mutation, where genotype is hardly predictive of phenotype.

Subcellular potassium and sodium distribution in Saccharomyces cerevisiae wild-type and vacuolar mutants

2013 ◽  
Vol 454 (3) ◽  
pp. 525-532 ◽  
Author(s):  
Rito Herrera ◽  
María C. Álvarez ◽  
Samuel Gelis ◽  
José Ramos
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Experimental Approach ◽  
Subcellular Location ◽  
Living Cells ◽  
Intracellular Distribution ◽  
Wild Type ◽  
Alkali Cations ◽  
Yeast Saccharomyces Cerevisiae ◽  
Multiple Functions ◽  
Plasma Membrane Permeabilization

Living cells accumulate potassium (K+) to fulfil multiple functions. It is well documented that the model yeast Saccharomyces cerevisiae grows at very different concentrations of external alkali cations and keeps high and low intracellular concentrations of K+ and sodium (Na+) respectively. However less attention has been paid to the study of the intracellular distribution of these cations. The most widely used experimental approach, plasma membrane permeabilization, produces incomplete results, since it usually considers only cytoplasm and vacuoles as compartments where the cations are present in significant amounts. By isolating and analysing the main yeast organelles, we have determined the subcellular location of K+ and Na+ in S. cerevisiae. We show that while vacuoles accumulate most of the intracellular K+ and Na+, the cytosol contains relatively low amounts, which is especially relevant in the case of Na+. However K+ concentrations in the cytosol are kept rather constant during the K+-starvation process and we conclude that, for that purpose, vacuolar K+ has to be rapidly mobilized. We also show that this intracellular distribution is altered in four different mutants with impaired vacuolar physiology. Finally, we show that both in wild-type and vacuolar mutants, nuclei contain and keep a relatively constant and important percentage of total intracellular K+ and Na+, which most probably is involved in the neutralization of negative charges.

Alkaloids of Crinum x powellii “Album” (Amaryllidaceae) and their Topoisomerase Inhibitory Activity

2007 ◽  
Vol 62 (3-4) ◽  
pp. 223-226 ◽  
Author(s):  
Jaime Niño ◽  
Gina M. Hincapié ◽  
Yaned M. Correa ◽  
Oscar M. Mosquera
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Inhibitory Activity ◽  
Topoisomerase I ◽  
Soluble Fraction ◽  
Genetically Engineered ◽  
Yeast Saccharomyces Cerevisiae ◽  
Spectroscopic Analyses ◽  
Nmr Techniques ◽  
First Time

Abstract The alkaloids lycorine, 1-O-acetyllycorine and ismine were isolated from the basic dichloromethane- soluble fraction of Crinum x powellii “Album” bulbs. The alkaloid structures were established by physical and spectroscopic analyses, including 1D NMR techniques and GCMS analysis. The three alkaloids are reported for the first time for this hybrid. Additionally, the three alkaloids isolated were tested against a mechanism-based bioassay utilizing genetically engineered mutants of the yeast Saccharomyces cerevisiae strains RAD+, RAD52Y and RS321 where lycorine was the only alkaloid that displayed moderate topoisomerase I inhibitory activity.

scholarly journals Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae

1999 ◽  
Vol 63 (2) ◽  
pp. 349-404 ◽  
Author(s):  
Frédéric Pâques ◽  
James E. Haber
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Genetic Recombination ◽  
Double Strand Breaks ◽  
Molecular Models ◽  
Yeast Saccharomyces Cerevisiae ◽  
Strand Breaks ◽  
The Past ◽  
Biochemical Studies

SUMMARY The budding yeast Saccharomyces cerevisiae has been the principal organism used in experiments to examine genetic recombination in eukaryotes. Studies over the past decade have shown that meiotic recombination and probably most mitotic recombination arise from the repair of double-strand breaks (DSBs). There are multiple pathways by which such DSBs can be repaired, including several homologous recombination pathways and still other nonhomologous mechanisms. Our understanding has also been greatly enriched by the characterization of many proteins involved in recombination and by insights that link aspects of DNA repair to chromosome replication. New molecular models of DSB-induced gene conversion are presented. This review encompasses these different aspects of DSB-induced recombination in Saccharomyces and attempts to relate genetic, molecular biological, and biochemical studies of the processes of DNA repair and recombination.

scholarly journals BIOSORBENTS BASED ON POLYSACCHARIDES. EVALUATION OF SORPTION CAPACITY IN RELATION TO URANIUM AND THORIUM

2019 ◽  
pp. 431-440
Author(s):  
Anatoliy Petrovich Karmanov ◽  
Al'bert Vladimirovich Kanarsky ◽  
Lyudmila Sergeyevna Kocheva ◽  
Zosia Al'bertovna Kanarskaya ◽  
Venera Maratovna Gematdinova ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sorption Capacity ◽  
Water Soluble ◽  
Yeast Saccharomyces Cerevisiae ◽  
High Sorption Capacity ◽  
Strong Adsorption ◽  
High Sorption ◽  
Physical Phenomena ◽  
First Time ◽  
The Relationship

Study of sorption of heavy natural radionuclide’s uranium and thorium from water by β-gluсancontaining sorbents obtained from biomass of yeast Saccharomyces Cerevisiae and bran of oat Avena sativa was carried out. It is shown that the content of mobile (water-soluble, exchange and acid-soluble) and fixed forms of uranium on investigated β-glucans vary considerably. It is found that the extent of irreversible sorption of uranium does not exceed 58.6%. For the first time shown that β-glucans have high sorption capacity in ratio of thorium. In the conditions of the experiments it was retrieved more than 99% of thorium from the water. The content of fixed form of thorium reaches 94% of the sorbed. Characteristics of surface and capillary-porous structure of samples were defined. The correlation relationships between rates of adsorption and specific surface of preparations were installed. An analysis of the relationship between sorption capacity and various properties of glucans leads to the conclusion that the most important role for the implementation of a strong adsorption of heavy radionuclides belongs to chemisorptions mechanisms, while the contribution of surface physical phenomena is not essential. It is shown that the highest strong adsorption of thorium is characterized by a sample representing the cell walls of yeast Saccharomyces cerevisiae. The findings suggest of β-glucans prospects in practical terms and their use as polyfunctional enterosorbеnts.

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