Drug resistance in yeast (Saccharomyces cerevisiæ)

1958 ◽  
Vol 48 (3) ◽  
pp. 109-113 ◽  
Author(s):  
T. R. Sharma ◽  
J. P. Shukla
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Yeast Saccharomyces Cerevisiae

scholarly journals The hidden complexity of Mendelian traits across yeast natural populations

2016 ◽  
Author(s):  
Jing Hou ◽  
Anastasie Sigwalt ◽  
David Pflieger ◽  
Jackson Peter ◽  
Jacky de Montigny ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Natural Population ◽  
Large Population ◽  
Natural Populations ◽  
Common Variants ◽  
Yeast Saccharomyces Cerevisiae ◽  
Inheritance Patterns ◽  
First Time ◽  
The Continuum

AbstractMendelian traits are considered as the lower end of the complexity spectrum of heritable phenotypes. However, more than a century after the rediscovery of Mendel’s law, the global landscape of monogenic variants as well as their effects and inheritance patterns within natural populations is still not well understood. Using the yeast Saccharomyces cerevisiae, we performed a species-wide survey of Mendelian traits across a large population of isolates. We generated offspring from 41 unique parental pairs, and analyzed 1,105 cross/trait combinations. We found that 8.9% of the cases were Mendelian. Most were caused by common variants showing stable inheritances in a natural population. However, we also found that a rare monogenic variant related to drug resistance displayed a significant and variable expressivity across different genetic backgrounds, leading to modified inheritances ranging from intermediate to high complexities. Our results illustrate for the first time the continuum of the hidden complexity of a monogenic mutation, where genotype is hardly predictive of phenotype.

Amiodarone inhibits multiple drug resistance in yeast Saccharomyces cerevisiae

2009 ◽  
Vol 191 (8) ◽  
pp. 675-679 ◽  
Author(s):  
Dmitry A. Knorre ◽  
Tatiana N. Krivonosova ◽  
Olga V. Markova ◽  
Fedor F. Severin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Multiple Drug Resistance ◽  
Multiple Drug ◽  
Yeast Saccharomyces Cerevisiae

scholarly journals Multiple-drug-resistance phenomenon in the yeast Saccharomyces cerevisiae: involvement of two hexose transporters.

1997 ◽  
Vol 17 (9) ◽  
pp. 5453-5460 ◽  
Author(s):  
A Nourani ◽  
M Wesolowski-Louvel ◽  
T Delaveau ◽  
C Jacq ◽  
A Delahodde
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Multidrug Resistance ◽  
Abc Transporters ◽  
Multiple Drug Resistance ◽  
Kluyveromyces Lactis ◽  
Major Facilitator Superfamily ◽  
Multiple Drug ◽  
Yeast Saccharomyces Cerevisiae ◽  
Hexose Transporters

In the yeast Saccharomyces cerevisiae, multidrug resistance to unrelated chemicals can result from overexpression of ATP-binding cassette (ABC) transporters such as Pdr5p, Snq2p, and Yor1p. Expression of these genes is under the control of two homologous zinc finger-containing transcription regulators, Pdr1p and Pdr3p. Here, we describe the isolation, by an in vivo screen, of two new Pdr1p-Pdr3p target genes: HXT11 and HXT9. HXT11 and HXT9, encoding nearly identical proteins, have a high degree of identity to monosaccharide transporters of the major facilitator superfamily (MFS). In this study, we show that the HXT11 product, which allows glucose uptake in a glucose permease mutant (rag1) strain of Kluyveromyces lactis, is also involved in the pleiotropic drug resistance process. Loss of HXT11 and/or HXT9 confers cycloheximide, sulfomethuron methyl, and 4-NQO (4-nitroquinoline-N-oxide) resistance. Conversely, HXT11 overexpression increases sensitivity to these drugs in the wild-type strain, an effect which is more pronounced in a strain having both PDR1 and PDR3 deleted. These data show that the two putative hexose transporters Hxt11p and Hxt9p are transcriptionally regulated by the transcription factors Pdr1p and Pdr3p, which are known to regulate the production of ABC transporters required for drug resistance in yeast. We thus demonstrate the existence of genetic interactions between genes coding for two classes of transporters (ABC and MFS) to control the multidrug resistance process.

Dodecyltriphenylphosphonium inhibits multiple drug resistance in the yeast Saccharomyces cerevisiae

2014 ◽  
Vol 450 (4) ◽  
pp. 1481-1484 ◽  
Author(s):  
Dmitry A. Knorre ◽  
Olga V. Markova ◽  
Ekaterina A. Smirnova ◽  
Iuliia E. Karavaeva ◽  
Svyatoslav S. Sokolov ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Multiple Drug Resistance ◽  
Multiple Drug ◽  
Yeast Saccharomyces Cerevisiae

PREPARASI DEINOCOCCUS RADIODURANS DAN KHAMIR DALAM MATERIAL KECAP L-DRYING SEBAGAI BAHAN UJI PROFISIENSI

2016 ◽  
Vol 13 (1) ◽  
pp. 93
Author(s):  
Titin Yulinery ◽  
Ratih M.Dewi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Deinococcus Radiodurans ◽  
Test Preparation ◽  
Quality Parameters ◽  
Soy Sauce ◽  
Yeast Saccharomyces Cerevisiae ◽  
Microbiological Test ◽  
Bacterial Contaminants ◽  
Minimum Number ◽  
Important Quality

Tes kemampuan adalah salah satu kegiatan penting dalam pengendalian mutu dan jaminan kualitas mikrobiologi laboratorium untuk mengukur kompetensi analis dan analisis uji profisiensi membutuhkan persiapan Model mikroorganisme adalah kualitas standar dan validitas. Mikrobiologi uji kualitas produk kedelai utama diarahkan pada kehadiran Saccharomyces cerevisiae ragi (S. cerevisiae), S. Bailli, S. rouxii dankontaminan bakteri seperti Bacillus dan Deinococcus. Jenis ragi dan bakteri yang terlibat dalam proses dan dapat menjadi salah satu parameter kualitas penting dalam persiapan yang dihasilkan. Jumlah dan viabilitas bakteri dan ragi menjadi parameter utama dalam proses persiapan bahan uji. Jumlah tersebut adalah jumlah minimum yang berlaku dapat dianalisis. Jumlah ini harus dibawah 10 CFU diperlukan untuk menunjukkan tingkat hygienitas proses dan tingkat minimal kontaminasi. Viabilitas bakteri dan bahan tes ragi persiapan untuk tes kemahiran kecap yang diawetkan dengan L-pengeringan adalah teknik Deinococcus radiodurans (D. radiodurans) 16 tahun, 58 tahun S. cerevisiae, dan S. roxii 13 tahun. kata kunci: Viabilitas, Deinococcus, khamir, L-pengeringan, Proficiency AbstractProficiency test is one of the important activities in quality control and quality assurance microbiology laboratory for measuring the competence of analysts and analysis Proficiency test requires a model microorganism preparations are standardized quality and validity. Microbiological test of the quality of the main soy products aimed at thepresence of yeast Saccharomyces cerevisiae (S. cerevisiae), S. bailli, S. rouxii and bacterial contaminants such as Bacillus and Deinococcus. Types of yeasts and bacteria involved in the process and can be one of the important quality parameters in the preparation produced. The number and viability of bacteria and yeasts become themain parameters in the process of test preparation materials. The amount in question is the minimum number that is valid can be analyzed. This amount must be below 10 CFU required to indicate the level of hygienitas process and the minimum level of contamination. Viability of bacteria and yeast test preparation materials for proficiencytest of soy sauce that preserved by L-drying technique is Deinococcus radiodurans ( D. radiodurans ) 16 years, 58 years S. cerevisiae, and S. roxii 13 years. key words : Viability, Deinococcus, Khamir, L-drying, Proficiency

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