scholarly journals Degradability, in vitro fermentation parameters and kinetic degradation of diets with increasing levels of forage and chitosan

2021 ◽  
Author(s):  
Amanna Gonzaga Jacaúna ◽  
Rafael Henrique de Tonissi e Buschinelli de Goes ◽  
Leonardo de Oliveira Seno ◽  
Luis Carlos Vinhas Ítavo ◽  
Jefferson Rodrigues Gandra ◽  
...  
Keyword(s):  
Degradation Kinetics ◽  
Block Design ◽  
Ammonia Concentration ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
In Vitro Fermentation ◽  
Randomized Block Design ◽  
Fermentation Parameters ◽  
Potential Use

Abstract Chitosan is the second most important natural biopolymer in the world, extracted from crustaceans, shrimps, and crabs; and can modulate rumen fermentation. Our hypothesis is that the addition of chitosan alters the fermentation patterns of different diets for ruminants. This study aimed to evaluate the effects of different levels of chitosan and forage on in vitro dry degradation kinetics and fermentation in a gas production system. The chitosan levels (0, 1625, 3500 or 7500 mg/kg of DM) were arranged in a completely randomized block design, and for in vitro ruminal fermentation assay we used a split splot arrangement. Into the incubator, all chitosan levels were distributed in the four jars, and the forage levels varying on 100, 65, 50, 35 and 20 on DM basis. Chitosan and roughage levels interaction effect (P≤0.05) on IVDMD; IVOMD. IVDCP and IVDNDF. Chitosan negatively affected IVDMD in all roughage levels evaluated. The pH and ammonia concentration present effect only for roughage levels and incubation hours. The chitosan didn’t change (P=0.3631) the total short-chain fatty acid concentration (overall mean = 21.19 mmol/L) and the C2:C3 ratio (overall mean = 5.85). The IVDCP showed the same decreasing quadratic behavior (P<0.0001). The increasing chitosan addition increases (P<0.0001) the gas production and decreases the (P<0.0001) the lag time (parameter C) of diets with greater concentrate participation, characterizing greater efficiency in the degradability of the diet, confirming its potential use in diets for ruminants. Chitosan changes in vitro dry degradation kinetics and fermentation at the minimum dose of 1722 mg/kg DM for all diets. The roughage level influenced the in vitro nutrients degradability and cumulative gas production.

312 Effects of Bismuth Subsalicylate and Different Sulfate Concentrations on in vitro Ruminal Fermentation Using a Warm-season Perennial Hay Substrate

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 166-166
Author(s):  
Kenneth S Madrid ◽  
Andrea M Osorio ◽  
Francine M Ciriaco ◽  
Kymberly D Coello ◽  
Angel A Raudales ◽  
...  
Keyword(s):  
Fixed Effects ◽  
Block Design ◽  
Random Effect ◽  
Gas Production ◽  
Experimental Unit ◽  
Ruminal Fermentation ◽  
In Vitro Fermentation ◽  
Bismuth Subsalicylate ◽  
H2s Production

Abstract A randomized complete block design was used to evaluate the effects of bismuth subsalicylate (BSS) on in vitro ruminal fermentation with differing concentrations of sulfate. In vitro fermentation consisted of 50 mL of a 4:1 buffer:ruminal fluid inoculum and 0.7 g (pre-dehydrated) of substrate [WW-B Dahl bluestem hay (Bothriochloa bladhii)] incubated for 48 h (39oC). Treatments were arranged as a 3 × 4 factorial with concentration of sulfate (0.2, 2.9, or 5.6 g sulfate/L buffer) and BSS (0.0, 0.165, 0.330, or 0.495% substrate DM) as the main factors. In vitro organic matter digestibility (IVOMD), and CH4, H2S, and total gas production (TGP) were measured. Data were analyzed using the MIXED procedure of SAS with the fixed effects of BSS, sulfate, and their interaction. Incubation day (block) was considered a random effect. The average of 2 bottles within day was considered experimental unit. A BSS × sulfate interaction was observed for TGP (P = 0.040) and H2S production (P < 0.001), where BSS had a larger negative impact on TGP and production of H2S with greater concentrations of sulfate. A linear effect (P < 0.001) of sulfate was observed for CH4 production per gram of incubated OM, where CH4 was decreased as sulfate concentration increased. A quadratic effect of sulfate was observed for IVOMD (P = 0.010) and pH (P = 0.009). Production of H2S linearly decreased (P = 0.001) as BSS concentration increased. The addition of BSS to in vitro incubations did not affect (P > 0.10) any other variables measured. Bismuth subsalicylate does not appear to have negative effects on in vitro fermentation parameters while decreasing H2S production; however, elevated concentrations of sulfate in the buffer appears to have negative impacts on fermentation. Further in vivo research is warranted to support BSS supplementation to cattle with high dietary sulfate.

332 Effect of Chemical and Biological Preservatives on in vitro Fermentation and Methane Production of Ensiled and Aerobically Exposed Wet Brewer’s Grains

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 182-182
Author(s):  
Marjorie A Killerby ◽  
Diego Zamudio ◽  
Kaycee Ames ◽  
Darren D Henry ◽  
Thomas Schwartz ◽  
...  
Keyword(s):  
Methane Production ◽  
Production System ◽  
Methane Concentration ◽  
Block Design ◽  
Gas Production ◽  
Sodium Lignosulfonate ◽  
In Vitro Fermentation ◽  
Randomized Complete Block Design ◽  
System Data

Abstract This study evaluated the effects of preservatives on the in vitro fermentation measures of wet brewer’s grain (WBG) silage at different stages of storage. Treatments (TRT) were sodium lignosulfonate at 1% (NaL1) and 2% (NaL2; w/w of fresh WBG), propionic acid (PRP; 0.5% w/w of fresh WBG), a combination inoculant (INO; Lactococcus lactis and Lactobacillus buchneri each at 4.9 log cfu/fresh WBG g), and untreated WBG (CON). WBG (Fresh) were packed into 8.8 L mini-silos and stored for 60 d at 21°C (Ensiled), then they were opened and aerobically exposed for 10d (AES). Samples from each stage of storage (STG; Fresh, Ensiled and AES) were analyzed for in vitro ruminal digestibility (24 h).Gas kinetics were recorded using the Ankom RF Gas Production System. Data were analyzed as a randomized complete block design (5 blocks) with a 5 (TRT) × 3 (STG) factorial arrangement. Apparent in vitro DM digestibility (DMD) decreased across STG, (51.5, 47.2 and 40.9 for Fresh, Ensiled and AES, respectively) and increased for NaL1, NaL2 and PRP (~47.8) vs. CON (43.0 ± 2.12%). PRP increased apparent in vitro OM digestibility (OMD) when Ensiled (54.5) and NaL2 increased it for AES (47.1) vs CON (46.3 and 39.9 ± 1.73%, respectively). The asymptotic maximal (M) and rate (k) of gas production decreased across STG (214.6, 181.5, 155.1 and 14.6, 12.6, and 9.8, for Fresh, Ensiled and AES, respectively). PRP increased (200.0) and NaL1 decreased (169.3) M vs. CON (183.9± 7.81ml/incubated DM g), while NaL1 and NaL2 (~11.4) decreased k vs. CON (13.4 ± 0.85%/h). Methane concentration and yield were higher in Fresh vs. other STG (0.94 vs. ~0.84 ± 0.07mM and 0.27 vs. ~0.23 ± 0.03mmol/g fermented OM). Spoilage of WBG decreases fermentability and methane production while PRP and NaL improve digestibility with the former also increasing M and k.

scholarly journals 409 Effects of essential oils on in vitro ruminal fermentation using a high-concentrate substrate

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 165-165
Author(s):  
Nadira J Espinoza-Rock ◽  
Andrea O Doblado ◽  
Sebastian E Mejia-Turcios ◽  
Evandro Dias ◽  
Michael Sandes ◽  
...  
Keyword(s):  
Essential Oils ◽  
Fixed Effects ◽  
Block Design ◽  
Random Effect ◽  
Gas Production ◽  
Experimental Unit ◽  
Ruminal Fermentation ◽  
Cinnamic Aldehyde ◽  
Garlic Oil

Abstract A randomized complete block design was used to determine the effects of 4 concentrations of 4 essential oils (EO) on in vitro ruminal fermentation variables. In vitro fermentation consisted of 0.7 g of high concentrate substrate (86.7% DM) and 50 mL of 2:1 buffer:ruminal fluid inoculum incubated for 24 h for each batch (n = 3; separate days) Treatments were arranged as a 4 × 5 factorial. Factors included 4 EO (eugenol, cinnamic aldehyde, anethole, and garlic oil) at 5 concentrations (0, 10, 75, 200, and 400 mg/L of inoculum). Data were analyzed using the MIXED procedure of SAS with the fixed effects of EO, concentration, and their interaction, and random effect of day (block). Batch was considered the experimental unit. There was an interaction (P < 0.001) for total gas production, where a cubic effect (P ≤ 0.041) was observed for eugenol, cinnamic aldehyde, and anethole, and a quadratic effect (P = 0.001) was observed for garlic oil. No interactions (P > 0.05) were observed for in vitro OM digestibility (IVOMD) or CH4 production. There was an effect of EO (P < 0.001) on IVOMD, where eugenol reduced (P ≤ 0.007) digestibility compared with anethole and garlic oil, which promoted the greatest (P ≤ 0.029) IVOMD. Methane production (mmol/g OM fermented) was affected by EO (P < 0.001), where it was decreased (P ≤ 0.001) by garlic oil compared with all other EO. There was an interaction (P < 0.001) for H2S production (µmol/g OM fermented), where it was linearly decreased (P = 0.003) and linearly increased (P < 0.001) as concentrations of eugenol and garlic oil increased, respectively. These EO had contradictory impacts on in vitro ruminal fermentation, thus combining them could potentially improve multiple aspects of in vitro and in vivo fermentation.

scholarly journals PSI-1 Effects of source and level of inclusion of engineered biocarbon in a total mixed beef cattle diet on in vitro methane emissions and fermentation parameters

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 290-291
Author(s):  
Paul Tamayao ◽  
Kim Ominski ◽  
Gabriel Ribeiro ◽  
Emma McGeough
Keyword(s):  
Fixed Effects ◽  
Pore Space ◽  
Block Design ◽  
In Vitro Study ◽  
Methane Emissions ◽  
Gas Production ◽  
Feed Digestibility ◽  
Fermentation Parameters ◽  
Barley Silage

Abstract This in vitro study evaluated seven different engineered biocarbon products supplied at three levels (0.5, 1.5 and 2.5 mg/ml inoculum) to determine their effects on total gas, methane production, and fermentation parameters when added to a barley silage-based diet. The biocarbon sources were derived from either coconut (CP001 and CP014) or pine (CP002, CP015, CP016, CP023, CP024) and differed in their physical properties and chemical composition. The coconut biocarbon sources were lower in pore space, particle size distribution and surface area but higher in bulk density than the pine products. The control consisted of only the barley-silage diet. The in vitro batch culture jars were incubated for 24 h at 39°C at the above inclusion levels in 0.5 g of diet. Gas samples were collected at 3, 6, 9, 12, 18 and 24 h and DM disappearance, pH, VFA and ammonia concentrations post incubation were measured. Data were analysed using the PROC MIXED in SAS as a randomized complete block design with treatment and rate as fixed effects and run and replicate as random effects. Total gas production was not affected by source of biocarbon (P = 0.85) and inclusion rate (P = 0.91). Cumulative methane (ml/g DM) had no response to biocarbon addition (P = 0.40) at any inclusion level (P = 0.48). Additionally, concentration of total VFA was not affected by treatment (P = 0.31) or inclusion rates (P = 0.25). NH3-N concentrations responded quadratically (P < 0.001) to all types of biocarbon. Higher inclusion rates of biocarbon linearly (P < 0.002) decreased feed digestibility, particularly the coconut-based biocarbon sources CP001 and CP014. In conclusion, supplementation of biocarbon to a TMR diet did not reduce methane emissions, but at higher levels of inclusion diet digestibility was negatively affected.

scholarly journals Fermentabilitas ruminal secara in vitro suplementasi tepung daun gamal, kelor, randu dan sengon dalam konsentrat hijau

2018 ◽  
Vol 28 (3) ◽  
pp. 213
Author(s):  
Eko Marhaeniyanto ◽  
Sri Susanti
Keyword(s):  
Crude Protein ◽  
Block Design ◽  
Gliricidia Sepium ◽  
Gas Production ◽  
Paraserianthes Falcataria ◽  
Leaf Meal ◽  
Ceiba Pentandra ◽  
Randomized Block Design

The aim of this research was to study the in vitro ruminal fermentability of supplementation of <em>Gliricidia sepium, Moringa oleifera</em>, Lamk (<em>MOL</em>), <em>Ceiba pentandra</em> and <em>Paraserianthes falcataria </em>leaf meal in concentrate feed to be tested on sheep.<strong> </strong>This research with the experimental methods was arranged in Randomized Block Design (RBD) with 6 treatments and 3 groups. The treatment feed tested consisted of concentrate without leaves and concentrate with leaf meal supplementation. Crude protein content (CP) concentrates were prepared 16%, 18% and 20%. Supplementation uses a mixture of <em>Gliricidia sepium, MOL</em>, <em>Ceiba pentandra</em> and <em>Paraserianthes falcataria </em>leaf meal in concentrate feed (1: 1: 1: 1) as much as 10%, 20% and 30%. The measured variables were  degradation of dry matter (DDM) and degradation of organic matter (DOM), gas production rate, microbial biomass and NH<sub>3</sub> concentration. The use of mixed leaf meal in concentrate feed resulted in in vitro DDM as well as concentrate feed without leaf meal supplementation. Supplementation of leaf meal in concentrate feed as much as 30% with CP 20% resulted in a decrease in the value of fermentability. The supplementation of leaf meal in concentrate feed as much as 20% in concentrate feed with CP 18% produced the best fermentability value. Suggested for in-vivo trials on sheep using 18% protein concentrate feed by utilizing <em>Gliricidia sepium, MOL</em>, <em>Ceiba pentandra</em> and <em>Paraserianthes falcataria </em>leaf meal in concentrate feed as cheap protein sources.

scholarly journals Characteristics of Ini Vitro Fermentative Digestibility of Odot Grass (Pennisetum Purpureum Cv. Mott) At Different Planting Spacing and Defoliation Age

2020 ◽  
pp. 368-376
Author(s):  
La Malesi ◽  
Takdir Saili ◽  
Ali Bain ◽  
Tresjia C Rakian
Keyword(s):  
Block Design ◽  
Animal Husbandry ◽  
Study Data ◽  
Nutrient Digestibility ◽  
Pennisetum Purpureum ◽  
In Vitro Digestibility ◽  
In Vitro Fermentation ◽  
Randomized Block Design ◽  
Ph Level

This study aimed to evaluate the characteristics of in vitro fermentative digestibility of odot grass planted at different spacing and defoliation ages-days after planting (DAP). The study was conducted in the Field Laboratory of the Faculty of Animal Science of the Halu Oleo University and the Laboratory of Nutrition for Dairy Sciences of the Faculty of Animal Husbandry, IPB University. The study was designed by using factorial randomized block design (3x3). The first factor was planting spacing (60 cm x 90 cm, 75 cm x 90 cm and 90 cm x 90 cm), and the second factor was defoliation age (60 DAP, 90 DAP, and 120 DAP), consisted of 4 groups and there were 36 treatment combinations. The grouping was based on the slope of the land. The study data were analyzed by using analysis of variance using SPSS 21 and if the treatment had a significant effect, a different test between treatments was tested by using the Duncan’s Multiple Range Test (DMRT) test. The experimental results showed that the interaction between planting spacing and defoliation age did not affect the characteristics of fermentation and nutrient digestibility in vitro. However, the in vitro fermentation characteristics were affected significantly (<em>p</em><0.05) by defoliation age, but were not affected by planting spacing whereas the pH level was not affected by spacing and defoliation age. Further tests of the differences between treatments showed that the planting spacing treatment was not significantly different (<em>p</em>>0.05), whereas among the defoliation age treatments were significantly different (<em>p</em><0.05). The conclusion of this study was the treatment of planting spacing and defoliation age did not affect digestibility in vitro fermentative of grass odot. The treatment of defoliation age independently influenced the in vitro digestibility of odot grass.

scholarly journals Effects of a tannin-rich legume (Onobrychis viciifolia) on in vitro ruminal biohydrogenation and fermentation

2016 ◽  
Vol 14 (1) ◽  
pp. e0602 ◽  
Author(s):  
Pablo G. Toral ◽  
Gonzalo Hervás ◽  
Hajer Missaoui ◽  
Sonia Andrés ◽  
Francisco J. Giráldez ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Ammonia Concentration ◽  
Gas Production ◽  
Composition Analysis ◽  
Milk Lipid ◽  
Ruminal Fermentation ◽  
Rumen Microorganisms ◽  
Onobrychis Viciifolia ◽  
Ruminal Biohydrogenation

<p>There is still controversy surrounding the ability of tannins to modulate the ruminal biohydrogenation (BH) of fatty acids (FA) and improve the lipid profile of milk or meat without conferring a negative response in the digestive utilization of the diet. Based on this, an <em>in vitro</em> trial using batch cultures of rumen microorganisms was performed to compare the effects of two legume hays with similar chemical composition but different tannin content, alfalfa and sainfoin (<em>Onobrychis viciifolia</em>), on the BH of dietary unsaturated FA and on the ruminal fermentation. The first incubation substrate, alfalfa, was practically free of tannins, while the second, sainfoin, contained 3.5% (expressed as tannic acid equivalents). Both hays were enriched with sunflower oil as a source of unsaturated FA. Most results of the lipid composition analysis (<em>e.g.</em>, greater concentrations of 18:2n-6, <em>cis</em>-9 18:1 or total polyunsaturated FA in sainfoin incubations) showed the ability of this tannin-containing legume to inhibit the BH process. However, no significant differences were detected in the accumulation of <em>cis</em>-9 <em>trans</em>-11 conjugated linoleic acid, and variations in <em>trans</em>-11 18:1 and<em> trans</em>-11 <em>cis</em>-15 18:2 did not follow a regular pattern. Regarding the rumen fermentation, gas production, ammonia concentration and volatile FA production were lower in the incubations with sainfoin (‒17, ‒23 and ‒11%, respectively). Thus, although this legume was able to modify the ruminal BH, which might result in improvements in the meat or milk lipid profile, the present results were not as promising as expected or as obtained before with other nutritional strategies.</p>

scholarly journals The Inclusion of Concentrate with Quebracho Is Advisable in Two Forage-Based Diets of Ewes According to the In Vitro Fermentation Parameters

Animals ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 451 ◽  
Author(s):  
Pablo Jose Rufino-Moya ◽  
Mireia Blanco ◽  
Sandra Lobón ◽  
Juan Ramon Bertolín ◽  
Ramón Armengol ◽  
...  
Keyword(s):  
Methane Production ◽  
Valeric Acid ◽  
Ruminal Fermentation ◽  
In Vitro Fermentation ◽  
N Content ◽  
Ch4 Production ◽  
Branched Chain ◽  
Fermentation Parameters ◽  
Forage Diets

Ewes receive hay or graze on fresh pastures supplemented with concentrates to fulfil their lactation requirements. Quebracho (Schinopsis balansae) can be added to change the ruminal fermentation. Fermentation parameters of forages alone and 70:30 forage:concentrate diets with control and quebracho concentrate were compared after 24 h of in vitro incubation. Fresh forage diets produced less gas (p < 0.05) and had greater IVOMD (p < 0.001), ammonia (NH3-N) content, valeric acid, branched-chain volatile fatty acid proportions, and lower propionic acid proportion than the hay diets (p < 0.01). In the hay diets, methane production increased with control concentrate (p < 0.01) and tended to decrease with quebacho concentrate (p < 0.10). The inclusion of both concentrates increased the acetic:propionic ratio (p < 0.01), and only the inclusion of quebracho concentrate increased the IVOMD (p < 0.01). In the fresh forage diets, gas and methane production increased with the inclusion of the control concentrate (p < 0.05), but methane production decreased with quebracho concentrate (p < 0.01). The inclusion of quebracho concentrate reduced the NH3-N content and valeric acid proportion (p < 0.05). In conclusion, the inclusion of quebracho concentrate would be advisable to reduce the CH4 production and NH3-N content in fresh forage diets and to increase the IVOMD in hay diets in comparison with the forages alone.

scholarly journals 100 Effects of bismuth subsalicylate and calcium- ammonium nitrate on in vitro fermentation of a high-concentrate substrate

2019 ◽  
Vol 97 (Supplement_1) ◽  
pp. 35-36
Author(s):  
Sebastian E Mejia-Turcios ◽  
Miranda K Stotz ◽  
Andrea M Osorio ◽  
Philip M Urso ◽  
Thomas G Jennings ◽  
...  
Keyword(s):  
Ammonium Nitrate ◽  
Random Effect ◽  
Gas Production ◽  
Feedlot Cattle ◽  
Feeding Strategies ◽  
Ruminal Fermentation ◽  
In Vitro Fermentation ◽  
Calcium Ammonium Nitrate ◽  
Bismuth Subsalicylate

Abstract An experiment was performed to determine the effects of bismuth subsalicylate (BSS) and calcium-ammonium nitrate (CAN) on in vitro fermentation of a high-concentrate (87% concentrate, DM basis) substrate. Serum bottles containing 20 mL of a 2:1 buffer:ruminal fluid inoculum and 0.2 g of substrate were incubated for 24 h. Four ruminally cannulated steers (BW = 520 ± 30 kg) were used as ruminal fluid donors and each donor was considered a block. Treatments were arranged in a 2 × 2 factorial with the following factors: BSS (0 or 0.33%, DM basis) and CAN (0 or 2.22%, DM basis). Treatments were made isonitrogenous with urea. In vitro organic matter digestibility (IVOMD) was determined in separate 100-mL centrifuge tubes. Data were analyzed using the MIXED procedure of SAS with the fixed effect of BSS, CAN, BSS × CAN, and the random effect of donor. An interaction (P < 0.01) was observed for total gas production (TGP). When CAN was included, without BSS, TGP was increased (P < 0.01); however, the combination of CAN with BSS did not affect (P = 0.85) TGP when compared to the combination of urea and BSS. Ammonia-N tended (P = 0.10) to increase when CAN was used as N source rather than urea. In vitro OM digestibility (P > 0.23) and final pH (P > 0.66) of in vitro ruminal fermentation were not affected by treatments. A tendency (P = 0.06) for an interaction regarding the production of H2S was observed; however, there were no treatment mean differences (P > 0.28). The combination of CAN and BSS did not negatively affect in vitro fermentation parameters such as OM digestion and gas production; however, a reduction in H2S with the combination of BSS and CAN may indicate potential benefits of such feeding strategies for feedlot cattle.

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