scholarly journals Characteristics of Ini Vitro Fermentative Digestibility of Odot Grass (Pennisetum Purpureum Cv. Mott) At Different Planting Spacing and Defoliation Age

2020 ◽  
pp. 368-376
Author(s):  
La Malesi ◽  
Takdir Saili ◽  
Ali Bain ◽  
Tresjia C Rakian
Keyword(s):  
Block Design ◽  
Animal Husbandry ◽  
Study Data ◽  
Nutrient Digestibility ◽  
Pennisetum Purpureum ◽  
In Vitro Digestibility ◽  
In Vitro Fermentation ◽  
Randomized Block Design ◽  
Ph Level

This study aimed to evaluate the characteristics of in vitro fermentative digestibility of odot grass planted at different spacing and defoliation ages-days after planting (DAP). The study was conducted in the Field Laboratory of the Faculty of Animal Science of the Halu Oleo University and the Laboratory of Nutrition for Dairy Sciences of the Faculty of Animal Husbandry, IPB University. The study was designed by using factorial randomized block design (3x3). The first factor was planting spacing (60 cm x 90 cm, 75 cm x 90 cm and 90 cm x 90 cm), and the second factor was defoliation age (60 DAP, 90 DAP, and 120 DAP), consisted of 4 groups and there were 36 treatment combinations. The grouping was based on the slope of the land. The study data were analyzed by using analysis of variance using SPSS 21 and if the treatment had a significant effect, a different test between treatments was tested by using the Duncan’s Multiple Range Test (DMRT) test. The experimental results showed that the interaction between planting spacing and defoliation age did not affect the characteristics of fermentation and nutrient digestibility in vitro. However, the in vitro fermentation characteristics were affected significantly (<em>p</em><0.05) by defoliation age, but were not affected by planting spacing whereas the pH level was not affected by spacing and defoliation age. Further tests of the differences between treatments showed that the planting spacing treatment was not significantly different (<em>p</em>>0.05), whereas among the defoliation age treatments were significantly different (<em>p</em><0.05). The conclusion of this study was the treatment of planting spacing and defoliation age did not affect digestibility in vitro fermentative of grass odot. The treatment of defoliation age independently influenced the in vitro digestibility of odot grass.

scholarly journals The Effect of Processing and Cooling Methods on Coleus tuberosus in vitro Starch Digestibility

2021 ◽  
Vol 41 (3) ◽  
pp. 238
Author(s):  
Jhauharotul Muchlisyiyah ◽  
Tri Dewanti Widyaningsih ◽  
Retno Wulansari ◽  
Hera Sisca Prasmita
Keyword(s):  
Resistant Starch ◽  
Room Temperature ◽  
Potato Starch ◽  
Block Design ◽  
In Vitro Digestibility ◽  
Randomized Block Design ◽  
Two Factors ◽  
Cooling Methods ◽  
Total Starch

Coleus tuberosus, also known as black potato, is one of the Indonesian local tubers consumed as a carbohydrate substituent. Therefore, this study aimed to examine the effect of processing and cooling methods on the in vitro digestibility of black potato starch. Furthermore, two factors Randomized Block Design with a 2x3 experimental design was used, which consisted of processing methods (boiling, roasting, and microwave) and cooling at room temperature and 4 °C for 24 hours with 3 repetitions. Black potato flour was compared with the raw form, by assessing some parameters, namely Resistant Starch (RS), Slowly Digestible Starch (SDS), Rapidly Digestible Starch (RDS), and Glycemic Index (GI). Also, the analysis of total starch, moisture, and color was performed, hence raw black potatoes generally have 10% resistant starch (%wb). Different treatments of cooking and cooling had a significant effect (α = 0.05) on moisture content, total starch, RS, RDS, SDS, GI, brightness (L), and yellowness (b). Black potatoes subjected to the processing method followed by cooling had lower RDS and increased RS content. Furthermore, refrigeration at 4°C for 24 hours reduced the digestibility of black potato starch more than cooling at room temperature. Contrarily, microwaved black potato cooled at room temperature showed a higher digestion rate compared to the raw counterpart. Conclusively, processing followed by cooling reduces the GI and increases the RS content of Coleus tuberosus.

scholarly journals In-vitro nutrient degradability of complete feed containing myristic acid and tannins addition

2022 ◽  
Vol 335 ◽  
pp. 00047
Author(s):  
Poespitasari Hazanah Ndaru ◽  
Siti Chuzaemi ◽  
Muhimmatu Mufidah
Keyword(s):  
Myristic Acid ◽  
Condensed Tannin ◽  
Block Design ◽  
Nutrient Content ◽  
In Vitro Digestibility ◽  
Corn Straw ◽  
Leaf Meal ◽  
Dry Matter Digestibility ◽  
Randomized Block Design

The purpose of this research was to determine the influence of Myristic acid and the source of condensed tannin on corn straw basedcomplete feed on nutrient content and in vitro digestibility. The method which was used in this experiment was randomized block design with four treatments and three replications. The treatment consisted of T0 complete feed (40% corn straw + 60% concentrate), T1 complete feed (40% corn straw + 60% concentrate + myristic acid 30 g/Kg DM), T2 complete feed (40% corn straw + 50% concentrate + calliandra leaf meal 10%/Kg DM and myristic acid 30 g/Kg DM), T3 complete feed (40% corn straw + 45% concentrate + calliandra leaf meal 15%/Kg DM and myristic acid 30 g/Kg DM), T4 (40% corn straw + 40% concentrate + calliandra leaf meal 20%/Kg DM and myristic acid 30 g/Kg DM). Based on the result, it could be concluded that calliandra leaf meal 15%/Kg DM + Myristic acid 30 g/Kg DM on the complete feed (T3) was the best treatment based on nutrient content and increase of dry matter digestibility, but not an effect of organic matter digestibility

scholarly journals In vitro evaluation of the association of chitosan and cashew nut shell liquid as additives for ruminants

2019 ◽  
Vol 20 ◽  
Author(s):  
Daniele Cristina PEREIRA ◽  
Rafael Henrique de Tonissi e Buschinelli de GOES ◽  
Antônio Campanha MARTINEZ ◽  
Jefferson Rodrigues GANDRA ◽  
Eduardo PRESENDO ◽  
...  
Keyword(s):  
Block Design ◽  
Ammonia Nitrogen ◽  
Animal Nutrition ◽  
In Vitro Digestibility ◽  
Cashew Nut Shell Liquid ◽  
In Vitro Evaluation ◽  
Cashew Nut ◽  
Randomized Block Design ◽  
Cashew Nut Shell

ABSTRACT This study evaluated the in vitro digestibility of nutrients from different diets added with chitosan (Q), technic cashew nut shell liquid (LCC) and the association of Q and LCC. The treatments used consisted of 4 diets (forage: concentrate ratio of 100: 0, 50:50, 40:60 and 20:80) associated with 4 additives (control, chitosan, LCC and the association of Q + LCC), totaling 16 treatments, in a 4x4 factorial randomized block design. The dosages used were: Control (without additives), LCC (600mg/kg DM), Chitosan (900mg/kg DM), and LCCQ (600mg/kg LCC DM + 900mg/kg Chitosan DM). In the laboratory, samples were analyzed for IVDMD, IVNDFD, IVCPD, pH and RAN (ruminal ammonia nitrogen). For pH and RAN analyses, samples were taken at 0, 2, 4, 6 and 8 hours after incubation. The results showed higher digestibility of DM, NDF and CP for diets with chitosan and technic cashew nut shell liquid alone and higher pH and RAN values in the diets containing the two additives. The association of additives brings better results for animal nutrition and increases ruminant productivity.

scholarly journals Influence of Different Vegetable Oils on In Vitro Ruminal Fermentability and Nutrient Digestibility in Ettawah Crossbred Goat

2020 ◽  
Vol 21 (1) ◽  
pp. 22
Author(s):  
Anis Muktiani ◽  
Nurul Arifah ◽  
Widiyanto Widiyanto
Keyword(s):  
Vegetable Oil ◽  
Palm Oil ◽  
Seed Oil ◽  
Corn Oil ◽  
Rumen Fluid ◽  
Block Design ◽  
Nutrient Digestibility ◽  
Dry Matter Digestibility ◽  
Randomized Block Design

This research was aimed to determine the effect of supplementation of vegetable oil (corn oil, palm oil and kapok seed oil) on in vitro ruminal fermentability and nutrient digestibility. Experiment design used was Randomized Block Design with four treatments and four replication based on rumen fluid sampling time. The four treatments namely R0 = feed without oil supplementation; R1 = R0 + 5% corn oil; R2 = R0 + 5% palm oil; and R3 = R0 + 5% kapok seed oil. The result showed that supplementation of 5% vegetable oil did not affect (P>0,05) the rumen pH, A/P ratio, and efficiency of energy conversion. The total VFA, acetate, propionate, butyrate, methane and NH3 was higher (P<0,05) in supplementation of 5% vegetable oil than he control. The population of protozoa, microbial protein, in vitro dry matter digestibility (IVDMD), organic matter digestibility (IVOMD), and crude fiber digestibility (IVCFD) was lower (P<0,05) in supplementation of 5% compared to the control. Population of protozoa decreased until 58,76% for R1; 66,89% for R2; and 43,33% for R3. It can be concluded that supplementation of 5% vegetable oil decreased the population of protozoa, increased the production of VFA and NH3. Supplementation of 5% kapok seed oil resulting the highest of total VFA, acetate, propionate, butyrate and NH3 among other treatments.

scholarly journals Degradability, in vitro fermentation parameters and kinetic degradation of diets with increasing levels of forage and chitosan

2021 ◽  
Author(s):  
Amanna Gonzaga Jacaúna ◽  
Rafael Henrique de Tonissi e Buschinelli de Goes ◽  
Leonardo de Oliveira Seno ◽  
Luis Carlos Vinhas Ítavo ◽  
Jefferson Rodrigues Gandra ◽  
...  
Keyword(s):  
Degradation Kinetics ◽  
Block Design ◽  
Ammonia Concentration ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
In Vitro Fermentation ◽  
Randomized Block Design ◽  
Fermentation Parameters ◽  
Potential Use

Abstract Chitosan is the second most important natural biopolymer in the world, extracted from crustaceans, shrimps, and crabs; and can modulate rumen fermentation. Our hypothesis is that the addition of chitosan alters the fermentation patterns of different diets for ruminants. This study aimed to evaluate the effects of different levels of chitosan and forage on in vitro dry degradation kinetics and fermentation in a gas production system. The chitosan levels (0, 1625, 3500 or 7500 mg/kg of DM) were arranged in a completely randomized block design, and for in vitro ruminal fermentation assay we used a split splot arrangement. Into the incubator, all chitosan levels were distributed in the four jars, and the forage levels varying on 100, 65, 50, 35 and 20 on DM basis. Chitosan and roughage levels interaction effect (P≤0.05) on IVDMD; IVOMD. IVDCP and IVDNDF. Chitosan negatively affected IVDMD in all roughage levels evaluated. The pH and ammonia concentration present effect only for roughage levels and incubation hours. The chitosan didn’t change (P=0.3631) the total short-chain fatty acid concentration (overall mean = 21.19 mmol/L) and the C2:C3 ratio (overall mean = 5.85). The IVDCP showed the same decreasing quadratic behavior (P&lt;0.0001). The increasing chitosan addition increases (P&lt;0.0001) the gas production and decreases the (P&lt;0.0001) the lag time (parameter C) of diets with greater concentrate participation, characterizing greater efficiency in the degradability of the diet, confirming its potential use in diets for ruminants. Chitosan changes in vitro dry degradation kinetics and fermentation at the minimum dose of 1722 mg/kg DM for all diets. The roughage level influenced the in vitro nutrients degradability and cumulative gas production.

Effects of a commercial blend of essential oils and monensin in a high-grain diet containing wheat distillers’ grains on in vitro fermentation

2013 ◽  
Vol 93 (3) ◽  
pp. 387-398 ◽  
Author(s):  
Y. L. Li ◽  
C. Li ◽  
K. A. Beauchemin ◽  
W. Z. Yang
Keyword(s):  
Essential Oils ◽  
Block Design ◽  
Neutral Detergent Fiber ◽  
Distillers Grains ◽  
Optimum Dose ◽  
In Vitro Fermentation ◽  
Randomized Block Design ◽  
Fermentation Pattern ◽  
In Vitro Effects

Li, Y. L., Li, C., Beauchemin, K. A. and Yang, W. Z. 2013. Effects of a commercial blend of essential oils and monensin in a high-grain diet containing wheat distillers’ grains on in vitro fermentation. Can. J. Anim. Sci. 93: 387–398. Our objective was to evaluate in vitro effects of a commercial blend of essential oils (BEO) versus monensin (MON) on fermentation of a high-grain diet containing wheat distillers’ dried grains with solubles (DDGS). Two experiments were conducted. The first experiment was designed as a short-term batch culture to determine the optimum dose of BEO (0, 45, 90 or 180 mg kg−1DM) on fermentation variables. The second experiment was a completely randomized block design with a 2×2 factorial arrangement of treatments using the rumen simulation technique. A high-grain dietary substrate containing wheat DDGS was supplemented with 0 or 28 mg MON kg−1DM combined with 0 and 90 mg BEO kg−1DM. Monensin did not affect the total volatile fatty acid (VFA) concentration or the molar proportions of individual VFA, but reduced CH4production (mL L−1gas; P=0.01) and disappearance of DM (P=0.04). Supplementation of BEO did not affect VFA concentration, but decreased (P=0.01) the molar proportion of acetate and reduced (P=0.02) the acetate to propionate concentration ratio and CH4production (mL g−1digested organic matter). The BEO increased (P=0.01) disappearance of neutral detergent fiber without affecting disappearance of other nutrients. These results indicate that supplementation of high-grain dietary substrate containing wheat DDGS with BEO improved fermentation pattern by increasing propionate concentration, reducing CH4production, and increasing fiber digestibility. This commercial BEO could be a potential substitute for MON for beef cattle fed high-grain diets containing DDGS.

scholarly journals Potential of methane and carbon dioxide in vitro production by Urochloa hybrid subjected to periods of coexistence with weeds

2020 ◽  
Vol 36 (3) ◽  
Author(s):  
Edenilson Meurer ◽  
Sara Carvalho de Brito ◽  
Sidnei Roberto de Marchi ◽  
Guilherme Henrique Rodrigues Pinheiro ◽  
Dagoberto Martins
Keyword(s):  
Nutritional Quality ◽  
Block Design ◽  
Seedling Emergence ◽  
In Vitro Digestibility ◽  
World Population ◽  
Ghg Emission ◽  
In Vitro Production ◽  
Potential Productivity ◽  
Randomized Block Design

The increased world population has raised the demand for animal proteins, forcing livestock to become productive, efficient, and environmentally sustainable. This study aimed to assess the effect of weed coexistence on productivity, nutritional quality, and potential production of greenhouse gases (GHG) by Convert HD364 grass (Urochloa hibrida cv. Mulato II) under pasture renovation conditions. The experiment was carried out in a randomized block design with four replications and treatments consisting of eight coexistence periods: 0, 15, 30, 45, 60, 75, 90, and 120 days after seedling emergence. Phytosociological assessments were performed in the weed community at the end of each coexistence period. The Convert HD364 grass was assessed for the morphological and chemical-bromatological parameters and for the GHG emission potential. Productivity, nutritional quality, and energy value of the Convert HD364 grass decreased proportionally with the increased period of weed coexistence. The non-coexistence with weeds results in a better in vitro digestibility of organic matter and, consequently, decreases the potential of GHG emission by the dry matter of the Convert HD364 grass.

scholarly journals Effect of rumen degradable protein and sulfur supplementation on in vitro digestibility and ruminal fermentation

2022 ◽  
Vol 951 (1) ◽  
pp. 012013
Author(s):  
A Rosmalia ◽  
Astriani ◽  
W P Sahroni ◽  
I G Permana ◽  
Despal
Keyword(s):  
Block Design ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Design Factor ◽  
Rumen Ph ◽  
Randomized Block Design ◽  
Two Factors ◽  
Total Bacteria ◽  
Degradable Protein

Abstract The availability of nitrogen and sulfur in the rumen should be synchronized to generate microbial protein synthesis. This study aimed to evaluate rumen degradable protein and sulfur supplementation on in vitro digestibility and ruminal fermentation. The experimental design was a 4 x 3 factorial randomized block design. Factor 1 was RDP levels (%CP) (R1= 60%; R2= 55%; R3= 50%; R4= 45%), and factor 2 was sulfur supplementation (S1= 0%; S2= 0.1%; S3= 0.2%). Data were analyzed with ANOVA followed by the Duncan test. The result showed that sulfur supplementation improved DMD and OMD without changes in rumen pH. NH3 concentration was influenced by RDP levels. Treatment R1 had the highest NH3 concentration (7.03 mM). There was an interaction of two factors on total VFA concentration. The combination of R1 and S2 resulted in the highest total VFA concentration than others. The total bacteria population increased with increasing RDP levels. Protozoa population was affected by RDP levels and sulfur supplementation. This study concluded that a combination of 60% RDP and 0.2% sulfur supplementation resulted in the best ration to improve ruminal fermentation and digestibility.

scholarly journals PSII-22 Effects of Clinoptilolite inclusion on Ruminant Invitro Digestibility

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 404-404
Author(s):  
Leeroy A Lente ◽  
Roderick A Gonzalez Murray ◽  
Shawn L Archibeque
Keyword(s):  
Dry Matter ◽  
Block Design ◽  
In Vitro Digestibility ◽  
Dry Matter Digestibility ◽  
Feed Digestibility ◽  
Randomized Block Design ◽  
Period Data ◽  
The Cost ◽  
In Vitro Rumen Fermentation

Abstract The aim of this study was to assess the effects of Zeolite (ZE, clinoptilolite) inclusion on in vitro rumen fermentation. A modified procedure from Tilley and Terry (1963) was used to determine alfalfa in vitro dry matter digestibility (IVDMD) in the presence or absence of ZE. Test tubes (n = 96) were placed in a 39 °C bath and were blocked based on hours 0, 1, 2, 4, 6, 8, 12, 24, and 48. Substrate consisted of dried alfalfa grinded through a 1mm screen using a Wily Mill. Treatments consisted of, Control (no ZE inclusion), and 0.05g ZE, for each 1 gram of alfalfa. After incubation, IVDMD was calculated 24 hours after drying period. Data was analyzed using a randomized block design. The IVDMD was found to be similar across all treatments (P &gt; 0.8464). The IVDMD was significantly different when incubated for the varying lengths of time (P &lt; 0.001). There was an IVDMD of 20.18 ± 2.89 % for the control and 18.07 ± 5.49 % for the ZE at the zero hour. The IVDMD for Control and ZE for 48 hours were 54.30 ± 1.58 % and 53. 48 ± 1.04 % respectively. To predict the IVDMD of the effects of ZE treatment on digestibility over time, the corresponding regression f(x) = 20.60 + 0.421X1 + 0.730X2 with R2 = 0.8464. In conclusion these data demonstrate that inclusion of ZE, does not influence the in vitro digestibility of alfalfa. These findings are important as previous data would indicate that inclusion of ZE may reduce cost of gain and/or influence liver abscess rates, it was uncertain if that would come at the cost of reduced digestibility of feed. These data indicate that there is likely very little to no impact on feed digestibility when ZE is included in the ration.

scholarly journals Effect of energy and protein level in complete feed used elephant grass (Pennisetum purpureum, Schum.) and maize stover (Zea mays. L) silage on nutrient content, total digestible nutrient, and in vitro degradation

2022 ◽  
Vol 335 ◽  
pp. 00027
Author(s):  
Hartutik ◽  
Marjuki ◽  
Asri Nurul Huda ◽  
Roi Katul Jannah
Keyword(s):  
Protein Level ◽  
Block Design ◽  
Descriptive Analysis ◽  
Nutrient Content ◽  
Pennisetum Purpureum ◽  
Elephant Grass ◽  
In Vitro Degradation ◽  
Maize Stover ◽  
Randomized Block Design

The purpose of this research was to determine of ideal ration of energy and protein in complete feed used elephant grass and maize stover silage. The materials were use elephant grass, maize stover silage with 10% molasses and Lactobacillus plantarum 1x106 CFU/g and concentrates. The method used experimental laboratory, the data of nutrient and TDN content using descriptive analysis. In vitro degradation value was analysed by Analysis of Variance from a factorial randomized block design and followed by Duncan’s Multiple Range Test. The complete feed was use 12.5% elephant grass + 37.5% maize stover silage + 50% concentrates with consist of energy level (E1 =12.5, E2 =13.5, E3 =14.5 MJ/kg DM) and protein level (P1 =10.5, P2= 13.5, P3= 16.5%). The results showed that in vitro DM and OM degradation respectively energy or protein level showed has significantly (P<0.01), while the interaction did not significant (P>0.05). The best treatment is E3P3 with energy 14.5 MJ/kg and protein 16.5% on nutrient content DM 92,51%., OM 90,33%., CP 16.57%, CF 19.29%, EE 1.77%, NFE 53.70%, TDN content 67.14%, In vitro DM degradation 66.14 % and in vitro OM degradation 70.01%.

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