scholarly journals 3D-printed gelatin methacrylate (GelMA)/silanated silica scaffold assisted by two-stage cooling system for hard tissue regeneration

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Eunjeong Choi ◽  
Dongyun Kim ◽  
Donggu Kang ◽  
Gi Hoon Yang ◽  
Bongsu Jung ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Tissue Regeneration ◽  
Cooling System ◽  
Composite Scaffold ◽  
Human Mesenchymal Stem Cells ◽  
Temperature Control System ◽  
Process Conditions ◽  
Hard Tissue ◽  
Two Stage ◽  
3D Printed

Abstract Among many biomaterials, gelatin methacrylate (GelMA), a photocurable protein, has been widely used in 3D bioprinting process owing to its excellent cellular responses, biocompatibility and biodegradability. However, GelMA still shows a low processability due to the severe temperature dependence of viscosity. To overcome this obstacle, we propose a two-stage temperature control system to effectively control the viscosity of GelMA. To optimize the process conditions, we evaluated the temperature of the cooling system (jacket and stage). Using the established system, three GelMA scaffolds were fabricated in which different concentrations (0, 3 and 10 wt%) of silanated silica particles were embedded. To evaluate the performances of the prepared scaffolds suitable for hard tissue regeneration, we analyzed the physical (viscoelasticity, surface roughness, compressive modulus and wettability) and biological (human mesenchymal stem cells growth, western blotting and osteogenic differentiation) properties. Consequently, the composite scaffold with greater silica contents (10 wt%) showed enhanced physical and biological performances including mechanical strength, cell initial attachment, cell proliferation and osteogenic differentiation compared with those of the controls. Our results indicate that the GelMA/silanated silica composite scaffold can be potentially used for hard tissue regeneration.

3D-printed alginate/phenamil composite scaffolds constituted with microsized core–shell struts for hard tissue regeneration

RSC Advances ◽  
2015 ◽  
Vol 5 (37) ◽  
pp. 29335-29345 ◽  
Author(s):  
KyoungHo Lee ◽  
Cho-Rong Seo ◽  
Jin-Mo Ku ◽  
Hyeongjin Lee ◽  
Hyeon Yoon ◽  
...  
Keyword(s):  
3D Printing ◽  
Tissue Regeneration ◽  
Composite Scaffold ◽  
Core Shell ◽  
Coating Process ◽  
Hard Tissue ◽  
Composite Scaffolds ◽  
Combined Process ◽  
3D Printed

A new composite scaffold consisting of poly(ε-caprolactone), alginate, and phenamil was manufactured by a combined process, 3D-printing and coating process, for hard tissue regeneration.

Behaviour of human mesenchymal stem cells on chemically synthesized HA-PCL scaffolds for hard tissue regeneration

2013 ◽  
Vol 10 (2) ◽  
pp. E147-E154 ◽  
Author(s):  
Vincenzo D'Antò ◽  
Maria Grazia Raucci ◽  
Vincenzo Guarino ◽  
Stefano Martina ◽  
Rosa Valletta ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Tissue Regeneration ◽  
Human Mesenchymal Stem Cells ◽  
Hard Tissue

scholarly journals Comparison of TCP and TCP/HA Hybrid Scaffolds for Osteoconductive Activity

2010 ◽  
Vol 4 (1) ◽  
pp. 279-285 ◽  
Author(s):  
P Wongwitwichot ◽  
J Kaewsrichan ◽  
K.H Chua ◽  
B.H.I Ruszymah
Keyword(s):  
Tissue Regeneration ◽  
Cell Attachment ◽  
Composite Scaffold ◽  
Porous Ceramic ◽  
Mass Ratio ◽  
Hard Tissue ◽  
Mixed Powder ◽  
The Matrix ◽  
Xrd Patterns ◽  
Hybrid Scaffolds

Two types of porous ceramic scaffolds were prepared, consisting of β-tricalcium phosphate (TCP) or the mixed powder of TCP and hydroxyapatite (HA) at a 2:1 mass ratio. A variety of methods have been used to fabricate bone scaffolds, while the sintering approach was adopted in this work. An extremely high temperature was used on sintering that proposed to consolidate the ceramic particles. As revealed by SEM, a well opened pore structure was developed within the scaffolds. The θ-values were measured to be of 73.3° and 6.5° for the composite scaffold and TCP sample, respectively. According to XRD patterns, the existence of grains coalescence and partial bonding between HA and TCP powders was demonstrated. Scaffold mechanical property in the term of flexural strength was also determined. The result showed decreasing of the strength by HA supplement, suggesting the more brittle characteristic of HA in comparison with TCP. By soaking the composite scaffold in PBS for a period of 2 weeks, transformation from particles to flank-like crystalline was clearly observed. Such change was found to be favorable for cell attachment, migration, and growth. By implanting cell-seeded scaffolds into nude mice, an abundant osseous extracellular matrix was identified for the composite implants. In contrast, the matrix was minimally detected in TCP implanted samples. Thus, the composite scaffold was found superior for hard tissue regeneration.

scholarly journals Effects of electromagnetic fields treatment on rat critical-sized calvarial defects with a 3D-printed composite scaffold

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Chang Tu ◽  
Jingyuan Chen ◽  
Chunwei Huang ◽  
Yifan Xiao ◽  
Xiangyu Tang ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Electromagnetic Fields ◽  
Composite Scaffold ◽  
Biomechanical Properties ◽  
Mitogen Activated Protein Kinase ◽  
High Porosity ◽  
Differentiation Capacity ◽  
3D Printed ◽  
Magnetic Therapy

Abstract Background Current strategies for craniofacial defect are faced with unmet outcome. Combining 3D-printing with safe, noninvasive magnetic therapy could be a promising breakthrough. Methods In this study, polylactic acid/hydroxyapatite (PLA/HA) composite scaffold was fabricated. After seeding rat bone marrow mesenchymal stem cells (BMSCs) on scaffolds, the effects of electromagnetic fields (EMF) on the proliferation and osteogenic differentiation capacity of BMSCs were investigated. Additionally, 6-mm critical-sized calvarial defect was created in rats. BMSC-laden scaffolds were implanted into the defects with or without EMF treatment. Results Our results showed that PLA/HA composite scaffolds exhibited uniform porous structure, high porosity (~ 70%), suitable compression strength (31.18 ± 4.86 MPa), modulus of elasticity (10.12 ± 1.24 GPa), and excellent cyto-compatibility. The proliferation and osteogenic differentiation capacity of BMSCs cultured on the scaffolds were enhanced with EMF treatment. Mechanistically, EMF exposure functioned partly by activating mitogen-activated protein kinase (MAPK) or MAPK-associated ERK and JNK pathways. In vivo, significantly higher new bone formation and vascularization were observed in groups involving scaffold, BMSCs, and EMF treatment, compared to scaffold alone. Furthermore, after 12 weeks of implanting, craniums in groups including scaffold, BMSCs, and EMF exposure showed the greatest biomechanical properties. Conclusion In conclusion, EMF treatment combined with 3D-printed scaffold has great potential applications in craniofacial regeneration.

In VivoComparison of Hard Tissue Regeneration with Human Mesenchymal Stem Cells Processed with Either the FICOLL Method or the BMAC Method

2010 ◽  
Vol 16 (2) ◽  
pp. 215-223 ◽  
Author(s):  
Sebastian Sauerbier ◽  
Andres Stricker ◽  
Jens Kuschnierz ◽  
Felicia Bühler ◽  
Toshiyuki Oshima ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Tissue Regeneration ◽  
Human Mesenchymal Stem Cells ◽  
Hard Tissue
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