3D-printed alginate/phenamil composite scaffolds constituted with microsized core–shell struts for hard tissue regeneration

RSC Advances ◽  
2015 ◽  
Vol 5 (37) ◽  
pp. 29335-29345 ◽  
Author(s):  
KyoungHo Lee ◽  
Cho-Rong Seo ◽  
Jin-Mo Ku ◽  
Hyeongjin Lee ◽  
Hyeon Yoon ◽  
...  
Keyword(s):  
3D Printing ◽  
Tissue Regeneration ◽  
Composite Scaffold ◽  
Core Shell ◽  
Coating Process ◽  
Hard Tissue ◽  
Composite Scaffolds ◽  
Combined Process ◽  
3D Printed

A new composite scaffold consisting of poly(ε-caprolactone), alginate, and phenamil was manufactured by a combined process, 3D-printing and coating process, for hard tissue regeneration.

scholarly journals 3D-printed gelatin methacrylate (GelMA)/silanated silica scaffold assisted by two-stage cooling system for hard tissue regeneration

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Eunjeong Choi ◽  
Dongyun Kim ◽  
Donggu Kang ◽  
Gi Hoon Yang ◽  
Bongsu Jung ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Tissue Regeneration ◽  
Cooling System ◽  
Composite Scaffold ◽  
Human Mesenchymal Stem Cells ◽  
Temperature Control System ◽  
Process Conditions ◽  
Hard Tissue ◽  
Two Stage ◽  
3D Printed

Abstract Among many biomaterials, gelatin methacrylate (GelMA), a photocurable protein, has been widely used in 3D bioprinting process owing to its excellent cellular responses, biocompatibility and biodegradability. However, GelMA still shows a low processability due to the severe temperature dependence of viscosity. To overcome this obstacle, we propose a two-stage temperature control system to effectively control the viscosity of GelMA. To optimize the process conditions, we evaluated the temperature of the cooling system (jacket and stage). Using the established system, three GelMA scaffolds were fabricated in which different concentrations (0, 3 and 10 wt%) of silanated silica particles were embedded. To evaluate the performances of the prepared scaffolds suitable for hard tissue regeneration, we analyzed the physical (viscoelasticity, surface roughness, compressive modulus and wettability) and biological (human mesenchymal stem cells growth, western blotting and osteogenic differentiation) properties. Consequently, the composite scaffold with greater silica contents (10 wt%) showed enhanced physical and biological performances including mechanical strength, cell initial attachment, cell proliferation and osteogenic differentiation compared with those of the controls. Our results indicate that the GelMA/silanated silica composite scaffold can be potentially used for hard tissue regeneration.

Engineering 3D printed bioactive composite scaffolds based on the combination of aliphatic polyester and calcium phosphates for bone tissue regeneration

2021 ◽  
Vol 122 ◽  
pp. 111928
Author(s):  
Eduardo H. Backes ◽  
Emanuel M. Fernandes ◽  
Gabriela S. Diogo ◽  
Catarina F. Marques ◽  
Tiago H. Silva ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Calcium Phosphates ◽  
Bone Tissue Regeneration ◽  
Aliphatic Polyester ◽  
Composite Scaffolds ◽  
3D Printed

scholarly journals Three-dimensionally printed polycaprolactone/multicomponent bioactive glass scaffolds for potential application in bone tissue engineering

2020 ◽  
Vol 6 (1) ◽  
pp. 57-69
Author(s):  
Amirhosein Fathi ◽  
Farzad Kermani ◽  
Aliasghar Behnamghader ◽  
Sara Banijamali ◽  
Masoud Mozafari ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Composite Scaffolds ◽  
3D Printed ◽  
Co Doped

AbstractOver the last years, three-dimensional (3D) printing has been successfully applied to produce suitable substitutes for treating bone defects. In this work, 3D printed composite scaffolds of polycaprolactone (PCL) and strontium (Sr)- and cobalt (Co)-doped multi-component melt-derived bioactive glasses (BGs) were prepared for bone tissue engineering strategies. For this purpose, 30% of as-prepared BG particles (size <38 μm) were incorporated into PCL, and then the obtained composite mix was introduced into a 3D printing machine to fabricate layer-by-layer porous structures with the size of 12 × 12 × 2 mm3.The scaffolds were fully characterized through a series of physico-chemical and biological assays. Adding the BGs to PCL led to an improvement in the compressive strength of the fabricated scaffolds and increased their hydrophilicity. Furthermore, the PCL/BG scaffolds showed apatite-forming ability (i.e., bioactivity behavior) after being immersed in simulated body fluid (SBF). The in vitro cellular examinations revealed the cytocompatibility of the scaffolds and confirmed them as suitable substrates for the adhesion and proliferation of MG-63 osteosarcoma cells. In conclusion, 3D printed composite scaffolds made of PCL and Sr- and Co-doped BGs might be potentially-beneficial bone replacements, and the achieved results motivate further research on these materials.

scholarly journals Comparison of TCP and TCP/HA Hybrid Scaffolds for Osteoconductive Activity

2010 ◽  
Vol 4 (1) ◽  
pp. 279-285 ◽  
Author(s):  
P Wongwitwichot ◽  
J Kaewsrichan ◽  
K.H Chua ◽  
B.H.I Ruszymah
Keyword(s):  
Tissue Regeneration ◽  
Cell Attachment ◽  
Composite Scaffold ◽  
Porous Ceramic ◽  
Mass Ratio ◽  
Hard Tissue ◽  
Mixed Powder ◽  
The Matrix ◽  
Xrd Patterns ◽  
Hybrid Scaffolds

Two types of porous ceramic scaffolds were prepared, consisting of β-tricalcium phosphate (TCP) or the mixed powder of TCP and hydroxyapatite (HA) at a 2:1 mass ratio. A variety of methods have been used to fabricate bone scaffolds, while the sintering approach was adopted in this work. An extremely high temperature was used on sintering that proposed to consolidate the ceramic particles. As revealed by SEM, a well opened pore structure was developed within the scaffolds. The θ-values were measured to be of 73.3° and 6.5° for the composite scaffold and TCP sample, respectively. According to XRD patterns, the existence of grains coalescence and partial bonding between HA and TCP powders was demonstrated. Scaffold mechanical property in the term of flexural strength was also determined. The result showed decreasing of the strength by HA supplement, suggesting the more brittle characteristic of HA in comparison with TCP. By soaking the composite scaffold in PBS for a period of 2 weeks, transformation from particles to flank-like crystalline was clearly observed. Such change was found to be favorable for cell attachment, migration, and growth. By implanting cell-seeded scaffolds into nude mice, an abundant osseous extracellular matrix was identified for the composite implants. In contrast, the matrix was minimally detected in TCP implanted samples. Thus, the composite scaffold was found superior for hard tissue regeneration.

scholarly journals Advances in 3D-Printed Surface-Modified Ca-Si Bioceramic Structures and Their Potential for Bone Tumor Therapy

Materials ◽  
2021 ◽  
Vol 14 (14) ◽  
pp. 3844
Author(s):  
Linh B. Truong ◽  
David Medina Cruz ◽  
Ebrahim Mostafavi ◽  
Catherine P. O’Connell ◽  
Thomas J. Webster
Keyword(s):  
Drug Delivery ◽  
3D Printing ◽  
Tissue Regeneration ◽  
Calcium Silicate ◽  
Mechanical Stability ◽  
Tumor Therapy ◽  
Base Material ◽  
Bone Cancer ◽  
Bone Tissue Regeneration ◽  
3D Printed

Bioceramics such as calcium silicate (Ca-Si), have gained a lot of interest in the biomedical field due to their strength, osteogenesis capability, mechanical stability, and biocompatibility. As such, these materials are excellent candidates to promote bone and tissue regeneration along with treating bone cancer. Bioceramic scaffolds, functionalized with appropriate materials, can achieve desirable photothermal effects, opening up a bifunctional approach to osteosarcoma treatments—simultaneously killing cancerous cells while expediting healthy bone tissue regeneration. At the same time, they can also be used as vehicles and cargo structures to deliver anticancer drugs and molecules in a targeted manner to tumorous tissue. However, the traditional synthesis routes for these bioceramic scaffolds limit the macro-, micro-, and nanostructures necessary for maximal benefits for photothermal therapy and drug delivery. Therefore, a different approach to formulate bioceramic scaffolds has emerged in the form of 3D printing, which offers a sustainable, highly reproducible, and scalable method for the production of valuable biomedical materials. Here, calcium silicate (Ca-Si) is reviewed as a novel 3D printing base material, functionalized with highly photothermal materials for osteosarcoma therapy and drug delivery platforms. Consequently, this review aims to detail advances made towards functionalizing 3D-printed Ca-Si and similar bioceramic scaffold structures as well as their resulting applications for various aspects of tumor therapy, with a focus on the external surface and internal dispersion functionalization of the scaffolds.

Engineering 3D-printed core-shell hydrogel scaffolds reinforced with hybrid hydroxyapatite/polycaprolactone nanoparticles for in vivo bone regeneration

2021 ◽  
Author(s):  
Salma Essam El-Habashy ◽  
Amal ElKamel ◽  
Marwa Essawy ◽  
Elsayeda-Zeinab Abdelfattah ◽  
Hoda M Eltaher
Keyword(s):  
3D Printing ◽  
Bone Regeneration ◽  
Composite Hydrogel ◽  
Core Shell ◽  
Hydrogel Scaffolds ◽  
3D Printed ◽  
Indispensable Tool

The versatility of 3D printing has rendered it an indispensable tool for the fabrication of composite hydrogel scaffolds, offering bone biomimetic features through inorganic and biopolymeric components as promising platforms...

scholarly journals 3D Printing of Alginate-Natural Clay Hydrogel-Based Nanocomposites

Gels ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 211
Author(s):  
Rebeca Leu Leu Alexa ◽  
Raluca Ianchis ◽  
Diana Savu ◽  
Mihaela Temelie ◽  
Bogdan Trica ◽  
...  
Keyword(s):  
3D Printing ◽  
Composite Scaffold ◽  
Infrared Spectrometry ◽  
Micro Computed Tomography ◽  
Buffer Solution ◽  
Nanocomposite Hydrogels ◽  
Crosslinking Process ◽  
Type Selection ◽  
Biological Studies ◽  
3D Printed

Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process makes alginate one of the most studied polysaccharides in the field of regenerative medicine. The main purpose of this study was to obtain tissue-like materials suitable for use in bone regeneration. In this respect, alginate and several types of clay were investigated as components of 3D-printing, nanocomposite inks. Using the extrusion-based nozzle, the nanocomposites inks were printed to obtain 3D multilayered scaffolds. To observe the behavior induced by each type of clay on alginate-based inks, rheology studies were performed on composite inks. The structure of the nanocomposites samples was examined using Fourier Transform Infrared Spectrometry and X-ray Diffraction (XRD), while the morphology of the 3D-printed scaffolds was evaluated using Electron Microscopy (SEM, TEM) and Micro-Computed Tomography (Micro-CT). The swelling and dissolvability of each composite scaffold in phosfate buffer solution were followed as function of time. Biological studies indicated that the cells grew in the presence of the alginate sample containing unmodified clay, and were able to proliferate and generate calcium deposits in MG-63 cells in the absence of specific signaling molecules. This study provides novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes, as well as valuable information on the clay type selection for enabling accurate 3D-printed constructs. Moreover, this study constitutes the first comprehensive report related to the screening of several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy.

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