scholarly journals A molecular beacon, bead-based assay for the detection of nucleic acids by flow cytometry

2005 ◽  
Vol 33 (2) ◽  
pp. e13-e13 ◽  
Author(s):  
D. Horejsh
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acids ◽  
Molecular Beacon

Flow Cytometry of Nucleic Acids

2000 ◽  
pp. 355-355
Author(s):  
Rochelle A. Diamond
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acids

A facile deoxyuridine/biotin-modified molecular beacon for simultaneous detection of proteins and nucleic acids via a label-free and background-eliminated fluorescence assay

The Analyst ◽  
2019 ◽  
Vol 144 (18) ◽  
pp. 5504-5510 ◽  
Author(s):  
Fei Yin ◽  
Liqi Liu ◽  
Xia Sun ◽  
Laiyong Hou ◽  
Yu Lu ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Nucleic Acids ◽  
Molecular Beacon ◽  
Simultaneous Detection ◽  
Cancer Biomarkers ◽  
Label Free ◽  
Early Diagnosis Of Cancer ◽  
Different Types ◽  
Diagnosis Of Cancer ◽  
Modified Molecular Beacon

Simultaneous detection of different types of cancer biomarkers (nucleic acids and proteins) could facilitate early diagnosis of cancer and clinical treatment.

scholarly journals Quantitative intracellular retention of delivered RNAs through optimized cell fixation and immuno-staining.

RNA ◽  
2021 ◽  
pp. rna.078895.121
Author(s):  
Prasath Paramasivam ◽  
Martin Stoter ◽  
Eloina Corradi ◽  
Irene Dalla Costa ◽  
Andreas Hoijer ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Single Molecule ◽  
Molecular Beacon ◽  
Cultured Cells ◽  
Endosomal Escape ◽  
Nucleic Acid Therapeutics ◽  
Optimized Protocol ◽  
Rna Probes ◽  
Cellular Compartments ◽  
Cell Fixation

Detection of nucleic acids within sub-cellular compartments is key to understanding their function. Determining the intracellular distribution of nucleic acids requires quantitative retention and estimation of their association with different organelles by immunofluorescence microscopy. This is particularly important for the delivery of nucleic acid therapeutics which depends on endocytic uptake and endosomal escape. However, the current protocols fail to preserve the majority of exogenously delivered nucleic acids in the cytoplasm. To solve this problem, by monitoring Cy5-labeled mRNA delivered to primary human adipocytes via lipid nanoparticles (LNP), we optimized cell fixation, permeabilization and immuno-staining of a number of organelle markers, achieving quantitative retention of mRNA and allowing visualization of levels which escape detection using conventional procedures. The optimized protocol proved effective on exogenously delivered siRNA, miRNA, as well as endogenous miRNA. Our protocol is compatible with RNA probes of single molecule fluorescence in-situ hybridization (smFISH) and molecular beacon, thus demonstrating that it is broadly applicable to study a variety of nucleic acids in cultured cells.

scholarly journals Correction: A facile deoxyuridine/biotin-modified molecular beacon for simultaneous detection of proteins and nucleic acids via a label-free and background-eliminated fluorescence assay

The Analyst ◽  
2020 ◽  
Vol 145 (22) ◽  
pp. 7448-7448
Author(s):  
Fei Yin ◽  
Liqi Liu ◽  
Xia Sun ◽  
Laiyong Hou ◽  
Yu Lu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Molecular Beacon ◽  
Simultaneous Detection ◽  
Fluorescence Assay ◽  
Label Free ◽  
Modified Molecular Beacon

Correction for ‘A facile deoxyuridine/biotin-modified molecular beacon for simultaneous detection of proteins and nucleic acids via a label-free and background-eliminated fluorescence assay’ by Fei Yin, et al., Analyst, 2019, 144, 5504–5510, DOI: 10.1039/C9AN01016E.

Real time monitoring of nucleic acids ligation based on molecular beacon

2003 ◽  
Vol 48 (12) ◽  
pp. 1215-1218 ◽  
Author(s):  
Zhiwen Tang ◽  
Kemin Wang ◽  
Weihong Tan ◽  
Jun Li ◽  
Lingfeng Liu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Real Time ◽  
Molecular Beacon ◽  
Real Time Monitoring ◽  
Nucleic Acids Ligation

A novel gold nanoparticles decorated magnetic microbead-based molecular beacon for DNA multiplexing detection by flow cytometry

2020 ◽  
Vol 1110 ◽  
pp. 19-25 ◽  
Author(s):  
Chengfei Li ◽  
Guangqiang Li ◽  
Huiqin Liu ◽  
Zhidong Xiao ◽  
Meilin Jin ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Gold Nanoparticles ◽  
Molecular Beacon ◽  
Magnetic Microbead
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