Real time monitoring of nucleic acids ligation based on molecular beacon

2003 ◽  
Vol 48 (12) ◽  
pp. 1215-1218 ◽  
Author(s):  
Zhiwen Tang ◽  
Kemin Wang ◽  
Weihong Tan ◽  
Jun Li ◽  
Lingfeng Liu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Real Time ◽  
Molecular Beacon ◽  
Real Time Monitoring ◽  
Nucleic Acids Ligation

Real-time monitoring of DNA polymerase activity using molecular beacon

2006 ◽  
Vol 353 (1) ◽  
pp. 141-143 ◽  
Author(s):  
Changbei Ma ◽  
Zhiwen Tang ◽  
Kemin Wang ◽  
Weihong Tan ◽  
Jun Li ◽  
...  
Keyword(s):  
Real Time ◽  
Dna Polymerase ◽  
Molecular Beacon ◽  
Polymerase Activity ◽  
Real Time Monitoring

scholarly journals Digital PCR - Methods and Protocols

2019 ◽  
Vol 63 (4) ◽  
Author(s):  
Paolo Catarsi
Keyword(s):  
Nucleic Acids ◽  
Real Time ◽  
Quantitative Pcr ◽  
Dna Amplification ◽  
Digital Pcr ◽  
Absolute Quantification ◽  
Relative Measurement ◽  
Real Time Monitoring ◽  
Major Step ◽  
Precision And Accuracy

Sensitivity, reproducibility, precision and accuracy are recurring words in this book. Indeed, digital PCR (dPCR) represents a major step forward in the quantification of nucleic acids. In this field, for many years it has been used the quantitative PCR (qPCR), a technology that allows a real-time monitoring of DNA amplification which has proved useful for obtaining a relative measurement, but that only indirectly, through the use of reference standards, provides an absolute quantification....

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