scholarly journals Spatial configuration of the chicken α-globin gene domain: immature and active chromatin hubs

2008 ◽  
Vol 36 (14) ◽  
pp. 4629-4640 ◽  
Author(s):  
Alexey A. Gavrilov ◽  
Sergey V. Razin
Keyword(s):  
Spatial Configuration ◽  
Globin Gene ◽  
Active Chromatin ◽  
Globin Gene Domain

scholarly journals The upstream area of the chicken α-globin gene domain is transcribed in both directions in the same cells

FEBS Letters ◽  
2005 ◽  
Vol 579 (21) ◽  
pp. 4746-4750 ◽  
Author(s):  
Victoria Borunova ◽  
Olga V. Iarovaia ◽  
Yegor S. Vassetzky ◽  
Sergey V. Razin
Keyword(s):  
Globin Gene ◽  
Globin Gene Domain

The coordinate replication of the human beta-globin gene domain reflects its transcriptional activity and nuclease hypersensitivity

1988 ◽  
Vol 8 (11) ◽  
pp. 4958-4965
Author(s):  
V Dhar ◽  
D Mager ◽  
A Iqbal ◽  
C L Schildkraut
Keyword(s):  
Cell Lines ◽  
Dna Sequences ◽  
Globin Gene ◽  
K562 Cells ◽  
Globin Genes ◽  
Beta Globin ◽  
Beta Globin Gene ◽  
Hypersensitive Sites ◽  
Flanking Sequences ◽  
Globin Gene Domain

The temporal order of replication of DNA sequences in the chromosomal domain containing the human beta-globin gene cluster and its flanking sequences (140 kilobases) was measured and compared in two different human cell lines. In human erythroleukemia (K562) cells, in which embryonic and fetal globin genes are transcribed, all of the sequences we examined from the beta-globin domain replicated early during S phase, while in HeLa cells, in which globin genes are transcriptionally silent, these sequences replicated late during S. Potential sites of initiation of DNA replication within this domain were identified. The beta-globin gene domain was also found to differ with respect to the nuclease sensitivity of the chromatin in these two cell lines. In K562 cells, hypersensitive sites for endogenous nucleases and DNase I were present in the chromatin near the earliest-replicating segments in the beta-globin domain.

scholarly journals The "beta-like-globin" gene domain in human erythroid cells.

1985 ◽  
Vol 82 (19) ◽  
pp. 6384-6388 ◽  
Author(s):  
D. Tuan ◽  
W. Solomon ◽  
Q. Li ◽  
I. M. London
Keyword(s):  
Globin Gene ◽  
Erythroid Cells ◽  
Globin Gene Domain

USF1 Recruits hSET1 Complex and Is Important for Maintaining Active Chromatin Domains in the β-Globin Locus.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 274-274
Author(s):  
Xingguo Li ◽  
Xin Hu ◽  
Gary Felsenfeld ◽  
Suming Huang
Keyword(s):  
Globin Gene ◽  
Critical Role ◽  
Leukemia Cell ◽  
Leukemia Cell Line ◽  
Chromatin Domain ◽  
Open Chromatin ◽  
Globin Genes ◽  
Active Chromatin ◽  
Insulator Element ◽  
Associated Proteins

Abstract The chicken β-globin insulator element acts as a barrier to the encroachment of chromosomal silencing. This barrier insulator prevents the spread of a 16 Kb domain of condensed chromatin which lies immediately upstream of the β-globin genes in the chicken genome. We have previously shown that the transcription factors USF1/2 mediate the barrier activity by binding to the 5′HS4 insulator element of the chicken β-globin locus and maintaining a local active chromatin structure. In the mouse β-globin locus, USFs also play a critical role in regulating the developmental stage-specific transcription of the β-major globin gene. To further understand the diverse roles of USF proteins, we undertook the biochemical isolation of USF1-containing multiprotein complexes. We found that USF1 associates with the hSET1 complex, which is an H3K4-specific methyltransferase. This complex exhibits histone H3 methyltransferase enzymatic activity. Consistent with the role of USF1 in recruiting histone modifying enzyme hSET1 to the 5′HS4 insulator, knockdown of USF1 expression in erythroid leukemia cell line 6C2 leads to the loss of histone H3K4 dimethylation at 5′HS4 of the chicken β-globin locus. In addition, USF1, hSET1, as well as H3K4 methylation are colocalized on HS2 of the locus control region (LCR) and the promoter of the β-major globin gene. Furthermore, the double ChIP analysis demonstrates that the histone methyltransferase hSET1 is recruited by transcription factor USF1 onto the mouse β-globin locus. The results suggest that hSET1 may play an important role in establishing H3K4 methylations on the active β-globin locus and reveal the mechanisms of USF and its associated proteins in maintaining the open chromatin domain and in regulation of globin gene transcription.

Detection of complementary transcripts for the intergenic region of the chicken α-globin gene domain

2015 ◽  
Vol 49 (6) ◽  
pp. 899-903
Author(s):  
E. S. Ioudinkova ◽  
A. V. Nefedochkina ◽  
O. V. Iarovaia ◽  
S. V. Razin
Keyword(s):  
Intergenic Region ◽  
Globin Gene ◽  
Globin Gene Domain

scholarly journals Organization of the 3′-boundary of the chicken α globin gene domain and characterization of a CR 1-specific protein binding site

1990 ◽  
Vol 18 (3) ◽  
pp. 401-409 ◽  
Author(s):  
Gabriel Farache ◽  
Sergey V. Razin ◽  
Félix Recillas Targa ◽  
Klaus Scherrer
Keyword(s):  
Protein Binding ◽  
Binding Site ◽  
Globin Gene ◽  
Specific Protein ◽  
Protein Binding Site ◽  
Globin Gene Domain
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