scholarly journals Organization of the 3′-boundary of the chicken α globin gene domain and characterization of a CR 1-specific protein binding site

1990 ◽  
Vol 18 (3) ◽  
pp. 401-409 ◽  
Author(s):  
Gabriel Farache ◽  
Sergey V. Razin ◽  
Félix Recillas Targa ◽  
Klaus Scherrer
Keyword(s):  
Protein Binding ◽  
Binding Site ◽  
Globin Gene ◽  
Specific Protein ◽  
Protein Binding Site ◽  
Globin Gene Domain

scholarly journals An NMR characterization of the regA protein-binding site of bacteriophage T4 gene 44 mRNA.

1991 ◽  
Vol 266 (27) ◽  
pp. 17832-17837
Author(s):  
A.A. Szewczak ◽  
K.R. Webster ◽  
E.K. Spicer ◽  
P.B. Moore
Keyword(s):  
Protein Binding ◽  
Binding Site ◽  
Bacteriophage T4 ◽  
Protein Binding Site ◽  
Nmr Characterization

scholarly journals Characterization of the S100A1 Protein Binding Site on TRPC6 C-Terminus

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e62677 ◽  
Author(s):  
Jan Bily ◽  
Lenka Grycova ◽  
Blanka Holendova ◽  
Michaela Jirku ◽  
Hana Janouskova ◽  
...  
Keyword(s):  
Protein Binding ◽  
Binding Site ◽  
Protein Binding Site ◽  
C Terminus

scholarly journals Functional homology between the yeast regulatory proteins GAL4 and LAC9: LAC9-mediated transcriptional activation in Kluyveromyces lactis involves protein binding to a regulatory sequence homologous to the GAL4 protein-binding site.

1987 ◽  
Vol 7 (12) ◽  
pp. 4400-4406 ◽  
Author(s):  
K D Breunig ◽  
P Kuger
Keyword(s):  
Protein Binding ◽  
Binding Site ◽  
Transcriptional Activation ◽  
Kluyveromyces Lactis ◽  
Regulatory Protein ◽  
Binding Activity ◽  
Protein Binding Site ◽  
Regulatory Sequence ◽  
Functional Homology ◽  
Beta Galactosidase

As shown previously, the beta-galactosidase gene of Kluyveromyces lactis is transcriptionally regulated via an upstream activation site (UASL) which contains a sequence homologous to the GAL4 protein-binding site in Saccharomyces cerevisiae (M. Ruzzi, K.D. Breunig, A.G. Ficca, and C.P. Hollenberg, Mol. Cell. Biol. 7:991-997, 1987). Here we demonstrate that the region of homology specifically binds a K. lactis regulatory protein. The binding activity was detectable in protein extracts from wild-type cells enriched for DNA-binding proteins by heparin affinity chromatography. These extracts could be used directly for DNase I and exonuclease III protection experiments. A lac9 deletion strain, which fails to induce the beta-galactosidase gene, did not contain the binding factor. The homology of LAC9 protein with GAL4 (J.M. Salmeron and S. A. Johnston, Nucleic Acids Res. 14:7767-7781, 1986) strongly suggests that LAC9 protein binds directly to UASL and plays a role similar to that of GAL4 in regulating transcription.

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