scholarly journals CTCF-dependent enhancer blockers at the upstream region of the chicken  -globin gene domain

2004 ◽  
Vol 32 (4) ◽  
pp. 1354-1362 ◽  
Author(s):  
V. Valadez-Graham
Keyword(s):  
Globin Gene ◽  
Upstream Region ◽  
Globin Gene Domain

scholarly journals The upstream area of the chicken α-globin gene domain is transcribed in both directions in the same cells

FEBS Letters ◽  
2005 ◽  
Vol 579 (21) ◽  
pp. 4746-4750 ◽  
Author(s):  
Victoria Borunova ◽  
Olga V. Iarovaia ◽  
Yegor S. Vassetzky ◽  
Sergey V. Razin
Keyword(s):  
Globin Gene ◽  
Globin Gene Domain

The coordinate replication of the human beta-globin gene domain reflects its transcriptional activity and nuclease hypersensitivity

1988 ◽  
Vol 8 (11) ◽  
pp. 4958-4965
Author(s):  
V Dhar ◽  
D Mager ◽  
A Iqbal ◽  
C L Schildkraut
Keyword(s):  
Cell Lines ◽  
Dna Sequences ◽  
Globin Gene ◽  
K562 Cells ◽  
Globin Genes ◽  
Beta Globin ◽  
Beta Globin Gene ◽  
Hypersensitive Sites ◽  
Flanking Sequences ◽  
Globin Gene Domain

The temporal order of replication of DNA sequences in the chromosomal domain containing the human beta-globin gene cluster and its flanking sequences (140 kilobases) was measured and compared in two different human cell lines. In human erythroleukemia (K562) cells, in which embryonic and fetal globin genes are transcribed, all of the sequences we examined from the beta-globin domain replicated early during S phase, while in HeLa cells, in which globin genes are transcriptionally silent, these sequences replicated late during S. Potential sites of initiation of DNA replication within this domain were identified. The beta-globin gene domain was also found to differ with respect to the nuclease sensitivity of the chromatin in these two cell lines. In K562 cells, hypersensitive sites for endogenous nucleases and DNase I were present in the chromatin near the earliest-replicating segments in the beta-globin domain.

scholarly journals The "beta-like-globin" gene domain in human erythroid cells.

1985 ◽  
Vol 82 (19) ◽  
pp. 6384-6388 ◽  
Author(s):  
D. Tuan ◽  
W. Solomon ◽  
Q. Li ◽  
I. M. London
Keyword(s):  
Globin Gene ◽  
Erythroid Cells ◽  
Globin Gene Domain

Juxtaposition of the HPFH2 Enhancer Is Not Sufficient To Reactivate the g-Globin Gene in Adult Erythropoiesis in bYAC Transgenic Mice.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 1215-1215
Author(s):  
Ping Xiang ◽  
Xin Ye ◽  
Hemei Han ◽  
Mary Stafford ◽  
Stamatoyannopoulos George ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Developmental Stages ◽  
Globin Gene ◽  
Fetal Hemoglobin ◽  
Upstream Region ◽  
Rnase Protection ◽  
Adult Mice ◽  
Gene Reactivation ◽  
Transgenic Lines ◽  
High Level

Abstract Hereditary persistence of fetal hemoglobin (HPFH) is characterized by high-level expression of the g globin gene in adult patients. HPFH2 contains a genetic deletion of approximately 83.5 kb from the middle of intron 2 of the yb gene to the region 66 kb 3′ to the b gene. The deletion juxtaposes an enhancer located downstream to the 3′ breakpoint to the vicinity of the g gene. To understand the reactivation mechanism of this fetal stage specific globin, we introduced the deletion into a 213 kb b-YAC. Four transgenic lines bearing 1–3 intact copies of the YAC construct were established. Globin gene expression at different developmental stages was measured by RNase protection assays. In the HPFH2 transgenic mice the e and g genes were expressed at high levels similar to the wild type YAC mice in embryonic and fetal stages. Unexpectedly, the g gene was completely silenced in the adult mice. The failure of g gene reactivation by juxtaposition of the HPFH2 enhancer contradicts the results reported by the Forget lab (Mol. Cell. Biol.17:2076–2089, 1997) and the Strouboulis lab (Blood102:3412–3419, 2003). The discrepancy could be due to the differences between the constructs used in the different labs. The construct used in the Forget lab was a cosmid containing the mLCR linked a 13 kb Gg and Ag fragment. The cosmid used by the Strouboulis lab contained the 22 kb LCR and a 5 kb Ag fragment. The bYAC construct we used in this study contains the whole b globin locus and spanned from about 40 kb 5′ to the e gene to 100 kb 3′ to the b gene. The failure of g gene reactivation in the HPFH2 YAC mice suggests that additional elements, which are missing in the 213 kb bYAC construct, are involved in reactivation of the g gene in the HPFH patients. Based on data from literature (Bender et al., Mol. Cell5:387–393, 2000; Forrester et al., Genes & Dev.4:1637–1649, 1990) and our own studies (Ping et al., this meeting) we speculate that the 200 kb upstream region is the candidate for harboring this activity.

Detection of complementary transcripts for the intergenic region of the chicken α-globin gene domain

2015 ◽  
Vol 49 (6) ◽  
pp. 899-903
Author(s):  
E. S. Ioudinkova ◽  
A. V. Nefedochkina ◽  
O. V. Iarovaia ◽  
S. V. Razin
Keyword(s):  
Intergenic Region ◽  
Globin Gene ◽  
Globin Gene Domain

scholarly journals Organization of the 3′-boundary of the chicken α globin gene domain and characterization of a CR 1-specific protein binding site

1990 ◽  
Vol 18 (3) ◽  
pp. 401-409 ◽  
Author(s):  
Gabriel Farache ◽  
Sergey V. Razin ◽  
Félix Recillas Targa ◽  
Klaus Scherrer
Keyword(s):  
Protein Binding ◽  
Binding Site ◽  
Globin Gene ◽  
Specific Protein ◽  
Protein Binding Site ◽  
Globin Gene Domain

scholarly journals The three-dimensional folding of the α-globin gene domain reveals formation of chromatin globules

2010 ◽  
Vol 18 (1) ◽  
pp. 107-114 ◽  
Author(s):  
Davide Baù ◽  
Amartya Sanyal ◽  
Bryan R Lajoie ◽  
Emidio Capriotti ◽  
Meg Byron ◽  
...  
Keyword(s):  
Globin Gene ◽  
Three Dimensional ◽  
Globin Gene Domain
Sign in / Sign up

Export Citation Format

Share Document