scholarly journals Endomembrane proteomics reveals putative enzymes involved in cell wall metabolism in wheat grain outer layers

2015 ◽  
Vol 66 (9) ◽  
pp. 2649-2658 ◽  
Author(s):  
Anne-Laure Chateigner-Boutin ◽  
Muhtadi Suliman ◽  
Brigitte Bouchet ◽  
Camille Alvarado ◽  
Virginie Lollier ◽  
...  
Keyword(s):  
Cell Wall ◽  
Wheat Grain ◽  
Cell Wall Metabolism

scholarly journals Cell Wall Proteome of Wheat Grain Endosperm and Outer Layers at Two Key Stages of Early Development

2019 ◽  
Vol 21 (1) ◽  
pp. 239 ◽  
Author(s):  
Cherkaoui Mehdi ◽  
Lollier Virginie ◽  
Geairon Audrey ◽  
Bouder Axelle ◽  
Larré Colette ◽  
...  
Keyword(s):  
Cell Wall ◽  
Protein Accumulation ◽  
Cell Wall Polysaccharide ◽  
Grain Development ◽  
Wheat Grain ◽  
Cell Wall Metabolism ◽  
Structural Cell ◽  
Stage Of Development ◽  
Proteomic Approach ◽  
Cell Wall Proteome

The cell wall is an important compartment in grain cells that fulfills both structural and functional roles. It has a dynamic structure that is constantly modified during development and in response to biotic and abiotic stresses. Non-structural cell wall proteins (CWPs) are key players in the remodeling of the cell wall during events that punctuate the plant life. Here, a subcellular and quantitative proteomic approach was carried out to identify CWPs possibly involved in changes in cell wall metabolism at two key stages of wheat grain development: the end of the cellularization step and the beginning of storage accumulation. Endosperm and outer layers of wheat grain were analyzed separately as they have different origins (maternal and seed) and functions in grains. Altogether, 734 proteins with predicted signal peptides were identified (CWPs). Functional annotation of CWPs pointed out a large number of proteins potentially involved in cell wall polysaccharide remodeling. In the grain outer layers, numerous proteins involved in cutin formation or lignin polymerization were found, while an unexpected abundance of proteins annotated as plant invertase/pectin methyl esterase inhibitors were identified in the endosperm. In addition, numerous CWPs were accumulating in the endosperm at the grain filling stage, thus revealing strong metabolic activities in the cell wall during endosperm cell differentiation, while protein accumulation was more intense at the earlier stage of development in outer layers. Altogether, our work gives important information on cell wall metabolism during early grain development in both parts of the grain, namely the endosperm and outer layers. The wheat cell wall proteome is the largest cell wall proteome of a monocot species found so far.

Cell wall metabolism during durian fruit dehiscence

2008 ◽  
Vol 48 (3) ◽  
pp. 391-401 ◽  
Author(s):  
L. Khurnpoon ◽  
J. Siriphanich ◽  
J.M. Labavitch
Keyword(s):  
Cell Wall ◽  
Cell Wall Metabolism ◽  
Fruit Dehiscence

scholarly journals Synergism of imipenem with fosfomycin associated with the active cell wall recycling and heteroresistance in Acinetobacter calcoaceticus-baumannii complex

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Uthaibhorn Singkham-in ◽  
Tanittha Chatsuwan
Keyword(s):  
Cell Wall ◽  
De Novo ◽  
Acinetobacter Calcoaceticus ◽  
Active Cell ◽  
Wild Type ◽  
Cell Wall Metabolism ◽  
Carbapenem Resistant ◽  
Type Form ◽  
Wild Type Form ◽  
Cell Wall Recycling

AbstractThe carbapenem-resistant Acinetobacter calcoaceticus-baumannii (ACB) complex has become an urgent threat worldwide. Here, we determined antibiotic combinations and the feasible synergistic mechanisms against three couples of ACB (A. baumannii (AB250 and A10), A. pittii (AP1 and AP23), and A. nosocomialis (AN4 and AN12)). Imipenem with fosfomycin, the most effective in the time-killing assay, exhibited synergism to all strains except AB250. MurA, a fosfomycin target encoding the first enzyme in the de novo cell wall synthesis, was observed with the wild-type form in all isolates. Fosfomycin did not upregulate murA, indicating the MurA-independent pathway (cell wall recycling) presenting in all strains. Fosfomycin more upregulated the recycling route in synergistic strain (A10) than non-synergistic strain (AB250). Imipenem in the combination dramatically downregulated the recycling route in A10 but not in AB250, demonstrating the additional effect of imipenem on the recycling route, possibly resulting in synergism by the agitation of cell wall metabolism. Moreover, heteroresistance to imipenem was observed in only AB250. Our results indicate that unexpected activity of imipenem on the active cell wall recycling concurrently with the presence of heteroresistance subpopulation to imipenem may lead to the synergism of imipenem and fosfomycin against the ACB isolates.

scholarly journals Comparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain

Planta ◽  
2014 ◽  
Vol 241 (3) ◽  
pp. 669-685 ◽  
Author(s):  
Richard Palmer ◽  
Valérie Cornuault ◽  
Susan E. Marcus ◽  
J. Paul Knox ◽  
Peter R. Shewry ◽  
...  
Keyword(s):  
Cell Wall ◽  
Wheat Grain ◽  
Cell Wall Matrix

scholarly journals Altered Mycobacterium tuberculosis Cell Wall Metabolism and Physiology Associated With RpoB Mutation H526D

2018 ◽  
Vol 9 ◽  
Author(s):  
Victoria L. Campodónico ◽  
Dalin Rifat ◽  
Yu-Min Chuang ◽  
Thomas R. Ioerger ◽  
Petros C. Karakousis
Keyword(s):  
Cell Wall ◽  
Mycobacterium Tuberculosis ◽  
Cell Wall Metabolism ◽  
Rpob Mutation
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