Physicochemical and molecular analysis of cell wall metabolism between two navel oranges (Citrus sinensis) with different mastication traits

2010 ◽  
Vol 90 (9) ◽  
pp. 1479-1484 ◽  
Author(s):  
Ying Lei ◽  
Yong-Zhong Liu ◽  
Wen-Fang Zeng ◽  
Xiu-Xin Deng
Keyword(s):  
Cell Wall ◽  
Molecular Analysis ◽  
Citrus Sinensis ◽  
Cell Wall Metabolism

scholarly journals Transcriptome Analysis Unravels Metabolic and Molecular Pathways Related to Fruit Sac Granulation in a Late-Ripening Navel Orange (Citrus sinensis Osbeck)

Plants ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 95 ◽  
Author(s):  
Li-Ming Wu ◽  
Ce Wang ◽  
Li-Gang He ◽  
Zhi-Jing Wang ◽  
Zhu Tong ◽  
...  
Keyword(s):  
Cell Wall ◽  
Low Temperature ◽  
Citrus Sinensis ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Winter Temperature ◽  
Soluble Solid Content ◽  
Navel Orange ◽  
Cell Wall Metabolism ◽  
Qrt Pcr

Lanelate navel orange (Citrus sinensis Osbeck) is a late-ripening citrus cultivar increasingly planted in China. The physiological disorder juice sac granulation often occurs in the fruit before harvest, but the physiological and molecular mechanisms underlying this disorder remain elusive. In this study, we found that fruit granulation of the late-ripening navel orange in the Three Gorges area is mainly caused by the low winter temperature in high altitude areas. Besides, dynamic changes of water content in the fruit after freezing were clarified. The granulation of fruit juice sacs resulted in increases in cell wall cellulose and decreases in soluble solid content, and the cells gradually became shrivelled and hollow. Meanwhile, the contents of pectin, cellulose, and lignin in juice sac increased with increasing degrees of fruit granulation. The activities of pectin methylesterase (PME) and the antioxidant enzymes peroxidase (POD), superoxide dismutase, and catalase increased, while those of polygalacturonase (PG) and cellulose (CL) decreased. Furthermore, a total of 903 differentially expressed genes were identified in the granulated fruit as compared with non-disordered fruit using RNA-sequencing, most of which were enriched in nine metabolic pathways, and qRT-PCR results suggested that the juice sac granulation is closely related to cell wall metabolism. In addition, the expression of PME involved in pectin decomposition was up-regulated, while that of PG was down-regulated. Phenylalanine ammonia lyase (PAL), cinnamol dehydrogenase (CAD), and POD related to lignin synthesis were up-regulated, while CL involved in cellulose decomposition was down-regulated. The expression patterns of these genes were in line with those observed in low-temperature treatment as revealed by qRT-PCR, further confirming that low winter temperature is associated with the fruit granulation of late-ripening citrus. Accordingly, low temperature would aggravate the granulation by affecting cell wall metabolism of late-ripening citrus fruit.

Cell wall metabolism during durian fruit dehiscence

2008 ◽  
Vol 48 (3) ◽  
pp. 391-401 ◽  
Author(s):  
L. Khurnpoon ◽  
J. Siriphanich ◽  
J.M. Labavitch
Keyword(s):  
Cell Wall ◽  
Cell Wall Metabolism ◽  
Fruit Dehiscence

scholarly journals Synergism of imipenem with fosfomycin associated with the active cell wall recycling and heteroresistance in Acinetobacter calcoaceticus-baumannii complex

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Uthaibhorn Singkham-in ◽  
Tanittha Chatsuwan
Keyword(s):  
Cell Wall ◽  
De Novo ◽  
Acinetobacter Calcoaceticus ◽  
Active Cell ◽  
Wild Type ◽  
Cell Wall Metabolism ◽  
Carbapenem Resistant ◽  
Type Form ◽  
Wild Type Form ◽  
Cell Wall Recycling

AbstractThe carbapenem-resistant Acinetobacter calcoaceticus-baumannii (ACB) complex has become an urgent threat worldwide. Here, we determined antibiotic combinations and the feasible synergistic mechanisms against three couples of ACB (A. baumannii (AB250 and A10), A. pittii (AP1 and AP23), and A. nosocomialis (AN4 and AN12)). Imipenem with fosfomycin, the most effective in the time-killing assay, exhibited synergism to all strains except AB250. MurA, a fosfomycin target encoding the first enzyme in the de novo cell wall synthesis, was observed with the wild-type form in all isolates. Fosfomycin did not upregulate murA, indicating the MurA-independent pathway (cell wall recycling) presenting in all strains. Fosfomycin more upregulated the recycling route in synergistic strain (A10) than non-synergistic strain (AB250). Imipenem in the combination dramatically downregulated the recycling route in A10 but not in AB250, demonstrating the additional effect of imipenem on the recycling route, possibly resulting in synergism by the agitation of cell wall metabolism. Moreover, heteroresistance to imipenem was observed in only AB250. Our results indicate that unexpected activity of imipenem on the active cell wall recycling concurrently with the presence of heteroresistance subpopulation to imipenem may lead to the synergism of imipenem and fosfomycin against the ACB isolates.

scholarly journals Altered Mycobacterium tuberculosis Cell Wall Metabolism and Physiology Associated With RpoB Mutation H526D

2018 ◽  
Vol 9 ◽  
Author(s):  
Victoria L. Campodónico ◽  
Dalin Rifat ◽  
Yu-Min Chuang ◽  
Thomas R. Ioerger ◽  
Petros C. Karakousis
Keyword(s):  
Cell Wall ◽  
Mycobacterium Tuberculosis ◽  
Cell Wall Metabolism ◽  
Rpob Mutation

scholarly journals Ozone Effects on Cell Wall Metabolism of Avena Coleoptile Sections

1964 ◽  
Vol 39 (5) ◽  
pp. 751-755 ◽  
Author(s):  
Lawrence Ordin ◽  
Betty P. Skoe
Keyword(s):  
Cell Wall ◽  
Cell Wall Metabolism ◽  
Avena Coleoptile

Role of ?-galactosidase in cell wall metabolism of date palm (Phoenix dactylifera) endosperm

PROTOPLASMA ◽  
1992 ◽  
Vol 166 (3-4) ◽  
pp. 177-186 ◽  
Author(s):  
Darleen A. DeMason ◽  
Monica A. Madore ◽  
K. N. Chandra Sekhar ◽  
Marilyn J. Harris
Keyword(s):  
Cell Wall ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Cell Wall Metabolism
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