scholarly journals Physcomitrella patens: a model to investigate the role of RAC/ROP GTPase signalling in tip growth

2010 ◽  
Vol 61 (7) ◽  
pp. 1917-1937 ◽  
Author(s):  
D. Magnus Eklund ◽  
Emma M. Svensson ◽  
Benedikt Kost
Keyword(s):  
Tip Growth ◽  
Physcomitrella Patens ◽  
Rop Gtpase

Microtubules and microfilaments in tip growth: evidence that microtubules impose polarity on protonemal growth in Physcomitrella patens

1988 ◽  
Vol 89 (4) ◽  
pp. 533-540 ◽  
Author(s):  
J. H. DOONAN ◽  
D. J. COVE ◽  
C. W. LLOYD
Keyword(s):  
Tip Growth ◽  
Physcomitrella Patens ◽  
Cytochalasin D ◽  
Immunofluorescence Study ◽  
Apical Dome ◽  
High Concentrations ◽  
Cytoskeletal Elements ◽  
Drug Studies ◽  
Tip Cells ◽  
Cytoskeletal Drugs

In this study we compare the contributions of Factin and microtubules to tip growth in filamentous cells of the moss Physcomitrella patens. In tip growth, expansion seems to be restricted to the hemispherical apical dome. Cytoskeletal elements have been suspected, from drug studies, to be involved in this but electron microscopy has generally not confirmed the presence of an apical cytoskeleton. However, in a previous immunofluorescence study we reported that microtubules could be seen to focus upon the apical dome in tip cells of the moss P. patens. In the present investigation F-actin has also been detected at the apices of these cells. Anti-cytoskeletal drugs were therefore used to differentiate between the roles of actin filaments and microtubules in tip growth. At high concentrations (30μM), the herbicide cremart de-polymerized microtubules and caused tip swelling. F-actin was still present under such conditions but its fragmentation by cytochalasin D suppressed this herbicide-induced swelling. On its own, cytochalasin D arrested tip growth without causing swollen tips. At lower concentrations, cremart disorganized microtubules rather than causing their complete depolymerization. Under these conditions, new but swollen growing points were initiated along the filament. The addition of taxol to cremart-treated filaments tended to reduce swelling and to re-polarize outgrowth. With particular combinations of these drugs, multiple lateral out-growths were initiated in the vicinity of the nucleus. It is concluded: (1) that F-actin is present at the tips of Physcomitrella caulonemal apical cells; (2) that unfragmented F-actin is necessary for outgrowth; (3) that even disorganized microtubules permit some degree of outgrowth but that an unperturbed distribution of axial microtubules, focussing upon an apex, is essential in order to impose tubular shape and directionality upon expansion.

scholarly journals Systematic survey of the function of ROP regulators and effectors during tip growth in the moss Physcomitrella patens

2018 ◽  
Vol 70 (2) ◽  
pp. 447-457 ◽  
Author(s):  
Carlisle Bascom ◽  
Graham M Burkart ◽  
Darren R Mallett ◽  
Jacquelyn E O’Sullivan ◽  
Alexis J Tomaszewski ◽  
...  
Keyword(s):  
Tip Growth ◽  
Physcomitrella Patens ◽  
Systematic Survey

scholarly journals Mapping the Growth of Fungal Hyphae: Orthogonal Cell Wall Expansion during Tip Growth and the Role of Turgor

2000 ◽  
Vol 79 (5) ◽  
pp. 2382-2390 ◽  
Author(s):  
Salomon Bartnicki-Garcia ◽  
Charles E. Bracker ◽  
Gerhard Gierz ◽  
Rosamaría López-Franco ◽  
Haisheng Lu
Keyword(s):  
Cell Wall ◽  
Tip Growth ◽  
Fungal Hyphae ◽  
Cell Wall Expansion

scholarly journals Cytokinins in the Bryophyte Physcomitrella patens: Analyses of Activity, Distribution, and Cytokinin Oxidase/Dehydrogenase Overexpression Reveal the Role of Extracellular Cytokinins

2007 ◽  
Vol 145 (3) ◽  
pp. 786-800 ◽  
Author(s):  
Klaus von Schwartzenberg ◽  
Marta Fernández Núñez ◽  
Hanna Blaschke ◽  
Petre I. Dobrev ◽  
Ondrej Novák ◽  
...  
Keyword(s):  
Physcomitrella Patens ◽  
Cytokinin Oxidase ◽  
Activity Distribution

Ultrastructure of sterigma growth and basidiospore formation in Coprinus and Boletus

1973 ◽  
Vol 51 (1) ◽  
pp. 145-150 ◽  
Author(s):  
David J. McLaughlin
Keyword(s):  
Golgi Apparatus ◽  
Tip Growth ◽  
Coprinus Cinereus ◽  
Additional Information ◽  
Cytoplasmic Vesicles

In basidia of Coprinus cinereus and Boletus rubinellus at early interphase II of meiosis numerous microtubules are oriented longitudinally in the sterigmata. Their orientation in the rest of the cell is predominantly parallel to the long axis. In Coprinus cinereus cytoplasmic vesicles, apparently derived from Golgi cisternae, are involved in tip growth of the sterigmata and basidiospore growth. Additional information is provided on the Golgi apparatus in the basidium, and the role of microtubules and microbodies is discussed.

scholarly journals Self-regulation in tip-growth: The role of cell wall ageing

2011 ◽  
Vol 283 (1) ◽  
pp. 113-121 ◽  
Author(s):  
Eelco Eggen ◽  
M. Niels de Keijzer ◽  
Bela M. Mulder
Keyword(s):  
Cell Wall ◽  
Tip Growth ◽  
Self Regulation
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