Comparative Insight into the Bacterial Communities in Alate and Apterous Morphs of Brown Citrus Aphid (Hemiptera: Aphididae)

2020 ◽  
Vol 113 (3) ◽  
pp. 1436-1444
Author(s):  
Feng Shang ◽  
Jinzhi Niu ◽  
Bi-Yue Ding ◽  
Jin-Jun Wang
Keyword(s):  
Bacterial Communities ◽  
Wing Dimorphism ◽  
Operational Taxonomic Units ◽  
Pseudomonas Species ◽  
Rrna Sequencing ◽  
High Throughput Dna Sequencing ◽  
The Difference ◽  
Tristeza Virus ◽  
Insight Into ◽  
Host Genes

Abstract Wing polyphenism (alate and apterous morphs) in aphids is a trade-off between dispersal and reproduction. How bacterial communities are associated with wing polyphenism in aphids is still not clearly understood. This study used 16S rRNA sequencing to examine the differences in diversity of the bacterial community between alate and apterous morphs in Aphis citricidus, the main vector of the Citrus tristeza virus. Eighty-one operational taxonomic units (OTUs) belonging to 37 orders, 34 classes, and 13 phyla were identified from all samples. Among these OTUs, Wolbachia (79.17%), Buchnera (17.64%), and Pseudomonas (2.99%) were the dominant bacterial genera. The diversity of symbionts varied between the two morphs; apterous morphs had more bacterial diversity (69 OTUs belonging to 45 families, 21 classes, and 12 phyla) than alate morphs (45 OTUs belonging to 36 families, 15 classes, and 10 phyla). In addition, the abundance of five OTUs was significantly different between two morphs. Among these OTUs, two Pseudomonas species (Pseudomonas_brenneri [OTU21] and unclassified_Pseudomonas [OTU13]) represented a high proportion (3.93% and 2.06%) in alate morphs but were present in low abundance (0.006% and 0.002%) in apterous morphs. RT-qPCR showed consistent results with high-throughput DNA sequencing. The preliminary survey showed the difference in composition and frequency of bacteria between alate and apterous morphs. Thus, the results contribute to anew insight of microorganisms that may be involved in wing dimorphism and helpful for controlling the dispersal of this pest through artificial elimination or reinfection of bacterial symbionts or targeting symbiosis-related host genes by RNA interference in future.

scholarly journals Bacterial diversity in snow from mid-latitude mountain areas: Alps, Eastern Anatolia, Karakoram and Himalaya

2018 ◽  
Vol 59 (77) ◽  
pp. 10-20 ◽  
Author(s):  
Roberto Sergio Azzoni ◽  
Ilario Tagliaferri ◽  
Andrea Franzetti ◽  
Christoph Mayer ◽  
Astrid Lambrecht ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Bacterial Communities ◽  
The Arctic ◽  
Eastern Anatolia ◽  
Mountain Areas ◽  
Air Mass ◽  
Back Trajectories ◽  
Air Masses ◽  
Operational Taxonomic Units ◽  
High Throughput Dna Sequencing

ABSTRACTSnow can be considered an independent ecosystem that hosts active microbial communities. Snow microbial communities have been extensively investigated in the Arctic and in the Antarctica, but rarely in mid-latitude mountain areas. In this study, we investigated the bacterial communities of snow collected in four glacierized areas (Alps, Eastern Anatolia, Karakoram and Himalaya) by high-throughput DNA sequencing. We also investigated the origin of the air masses that produced the sampled snowfalls by reconstructing back-trajectories. A standardized approach was applied to all the analyses in order to ease comparison among different communities and geographical areas. The bacterial communities hosted from 25 to 211 Operational Taxonomic Units (OTUs), and their structure differed significantly between geographical areas. This suggests that snow bacterial communities may largely derive from ‘local’ air bacteria, maybe by deposition of airborne particulate of local origin that occurs during snowfall. However, some evidences suggest that a contribution of bacteria collected during air mass uplift to snow communities cannot be excluded, particularly when the air mass that originated the snow event is particularly rich in dust.

scholarly journals Bacterial communities associated with individual transparent exopolymer particles (TEP)

2019 ◽  
Vol 41 (4) ◽  
pp. 561-565 ◽  
Author(s):  
Birthe Zäncker ◽  
Anja Engel ◽  
Michael Cunliffe
Keyword(s):  
Organic Matter ◽  
Bacterial Community ◽  
Dna Sequencing ◽  
Bacterial Communities ◽  
Bacterial Community Composition ◽  
Euphotic Zone ◽  
Carbon Export ◽  
Transparent Exopolymer Particles ◽  
Operational Taxonomic Units ◽  
Insight Into

Abstract Transparent exopolymer particles (TEP) are polysaccharide-rich microgels that are prevalent in the marine environment and have important roles in the aggregation of organic matter and carbon export from the euphotic zone. TEP are readily colonized by bacteria and utilized by specialized taxa, such as Alteromonadaceae. However, bacterial community composition specifically attached to natural TEP remains largely unknown. In this study, we isolated individual TEP from Plymouth Sound (UK) and performed DNA sequencing of the TEP-attached bacterial communities. We also sampled the cognate bulk seawater total bacterial communities for comparison. The bacterial communities associated with individual TEP showed distinct differences compared to the total bulk bacterioplankton communities, with Alteromonadaceae significantly more abundant on TEP. The TEP-associated Alteromonadaceae consisted of two operational taxonomic units that were closely related to Marinobacter and Glaciecola, both previously associated with biogenic aggregates and microgel-rich habitats. This study provides novel insight into marine bacterial–microgel interactions.

scholarly journals Functional genomics study of Pseudomonas putida to determine traits associated with avoidance of a myxobacterial predator

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shukria Akbar ◽  
D. Cole Stevens
Keyword(s):  
Pseudomonas Putida ◽  
Bacterial Communities ◽  
Predator Avoidance ◽  
Phenotypic Diversity ◽  
Myxococcus Xanthus ◽  
Predator Prey ◽  
Mucoid Conversion ◽  
Small Colony ◽  
Predator Prey Models ◽  
Insight Into

AbstractPredation contributes to the structure and diversity of microbial communities. Predatory myxobacteria are ubiquitous to a variety of microbial habitats and capably consume a broad diversity of microbial prey. Predator–prey experiments utilizing myxobacteria have provided details into predatory mechanisms and features that facilitate consumption of prey. However, prey resistance to myxobacterial predation remains underexplored, and prey resistances have been observed exclusively from predator–prey experiments that included the model myxobacterium Myxococcus xanthus. Utilizing a predator–prey pairing that instead included the myxobacterium, Cystobacter ferrugineus, with Pseudomonas putida as prey, we observed surviving phenotypes capable of eluding predation. Comparative transcriptomics between P. putida unexposed to C. ferrugineus and the survivor phenotype suggested that increased expression of efflux pumps, genes associated with mucoid conversion, and various membrane features contribute to predator avoidance. Unique features observed from the survivor phenotype when compared to the parent P. putida include small colony variation, efflux-mediated antibiotic resistance, phenazine-1-carboxylic acid production, and increased mucoid conversion. These results demonstrate the utility of myxobacterial predator–prey models and provide insight into prey resistances in response to predatory stress that might contribute to the phenotypic diversity and structure of bacterial communities.

scholarly journals New Insights on the Zeugodacus cucurbitae (Coquillett) Bacteriome

2021 ◽  
Vol 9 (3) ◽  
pp. 659
Author(s):  
Elias Asimakis ◽  
Panagiota Stathopoulou ◽  
Apostolis Sapounas ◽  
Kanjana Khaeso ◽  
Costas Batargias ◽  
...  
Keyword(s):  
Relative Abundance ◽  
Bacterial Communities ◽  
High Throughput Sequencing ◽  
Mutual Exclusion ◽  
Bacterial Composition ◽  
Operational Taxonomic Units ◽  
Host Diet ◽  
Dominant Bacteria ◽  
High Degree

Various factors, including the insect host, diet, and surrounding ecosystem can shape the structure of the bacterial communities of insects. We have employed next generation, high-throughput sequencing of the 16S rRNA to characterize the bacteriome of wild Zeugodacus (Bactrocera) cucurbitae (Coquillett) flies from three regions of Bangladesh. The tested populations developed distinct bacterial communities with differences in bacterial composition, suggesting that geography has an impact on the fly bacteriome. The dominant bacteria belonged to the families Enterobacteriaceae, Dysgomonadaceae and Orbaceae, with the genera Dysgonomonas, Orbus and Citrobacter showing the highest relative abundance across populations. Network analysis indicated variable interactions between operational taxonomic units (OTUs), with cases of mutual exclusion and copresence. Certain bacterial genera with high relative abundance were also characterized by a high degree of interactions. Interestingly, genera with a low relative abundance like Shimwellia, Gilliamella, and Chishuiella were among those that showed abundant interactions, suggesting that they are also important components of the bacterial community. Such knowledge could help us identify ideal wild populations for domestication in the context of the sterile insect technique or similar biotechnological methods. Further characterization of this bacterial diversity with transcriptomic and metabolic approaches, could also reveal their specific role in Z. cucurbitae physiology.

HYPOCHROMIC MICROCYTIC ANEMIA OF INFANCY: IRON DEPLETION AS A FACTOR

PEDIATRICS ◽  
1956 ◽  
Vol 18 (6) ◽  
pp. 959-978
Author(s):  
Hugh W. Josephs
Keyword(s):  
Iron Deficiency ◽  
Severe Infection ◽  
Microcytic Anemia ◽  
Severe Anemia ◽  
Iron Depletion ◽  
Hypochromic Microcytic Anemia ◽  
Premature Babies ◽  
Number Of Factors ◽  
The Difference ◽  
Insight Into

In this work the author has attempted to gain insight into the significance of iron depletion by the use of 4 simple calculations, justification for which is found in recent articles. These are: (a) iron with which the infant is born; (b) iron retained from the food; (c) iron being used by the tissues and therefore unavailable for hemoglobin, and (d) iron combined with the total mass of hemoglobin. With these 4 figures it is possible to estimate the iron still potentially available for use (the "reserves" or "stones"). When the difference between a + b and c + d has reached about zero, depletion is considered to exist. The following characteristics of depletion may be emphasized: Depletion is the result of gain in weight and maximum possible usage of iron. It is therefore a normal result of growth and need not be associated with anemia. As soon as depletion has occurred, the organism is thereafter dependent on current absorption of iron. This is ordinarily sufficient, even with a diet of milk alone, to maintain an adequate concentration of hemoglobin after about 8 to 10 months of age. Severe anemia due to depletion alone is practically confined to premature babies whose relative gain in weight is rapid. Severe anemia in other than premature babies is the result of a number of factors by which iron becomes unavailable or is actually diverted from hemoglobin to storage. Response to iron medication is considerably better in infants with depletion than in those in whom some factor is present that interferes with iron utilization, and which is not connected by the mere giving of iron. The dependence on current absorption, whether the result of depletion or non-availability, introduces a certain precariousness which is apparently characteristic of this time of life. The organism gets along from day to day if nothing happens, but may not be able to meet an emergency, whether this appears as a rapid gain in weight, or a necessity to repair damage done by severe infection. If we consider iron deficiency as the cause of anemia, we can think of deficiency as due to a number of factors of which depletion is only one. The development and characteristics of depletion have been considered in this paper; other factors in iron deficiency will be considered in subsequent papers.

scholarly journals Research benefits of storing genitourinary samples: 16S rRNA sequencing to evaluate vaginal bacterial communities

2017 ◽  
Vol 28 (3) ◽  
pp. 315-317 ◽  
Author(s):  
Josef Wagner ◽  
Sarah Kerry-Barnard ◽  
ST Sadiq ◽  
Pippa Oakeshott
Keyword(s):  
16S Rrna ◽  
Bacterial Communities ◽  
16S Rrna Sequencing ◽  
Rrna Sequencing

scholarly journals Pseudomonas-Specific NGS Assay Provides Insight Into Abundance and Dynamics of Pseudomonas Species Including P. aeruginosa in a Cooling Tower

2018 ◽  
Vol 9 ◽  
Author(s):  
Rui P. A. Pereira ◽  
Jörg Peplies ◽  
Douglas Mushi ◽  
Ingrid Brettar ◽  
Manfred G. Höfle
Keyword(s):  
Cooling Tower ◽  
Pseudomonas Species ◽  
Insight Into

scholarly journals Experimental Study on the Transition between Static and Kinetic Frictions of Steel/Shale Pairs

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Qin Lian ◽  
Chunxu Yang ◽  
Jifei Cao
Keyword(s):  
Friction Coefficient ◽  
Normal Load ◽  
Critical Distance ◽  
Dynamic Friction ◽  
Stick Slip ◽  
Dynamic Friction Coefficient ◽  
The Coefficient Of Friction ◽  
The Difference ◽  
Stick Slip Motion ◽  
Insight Into

The transition between static and kinetic frictions of steel/shale pairs has been studied. It was found that the coefficient of friction decreased exponentially from static to dynamic friction coefficient with increasing sliding displacement. The difference between static and dynamic friction coefficients and the critical distance Dc under the dry friction condition is much larger than that under the lubricated condition. The transition from static to dynamic friction coefficient is greatly affected by the normal load, quiescent time, and sliding velocity, especially the lubricating condition. Maintaining continuous lubrication of the contact area by the lubricant is crucial to reduce or eliminate the stick-slip motion. The results provide an insight into the transition from static to dynamic friction of steel/shale pairs.

scholarly journals An Insight Into the Value Added Tax System Detailing Its Origins, Impact, Provisions and Regulations With Reference to India

2004 ◽  
Vol 3 (1) ◽  
pp. 19-29
Author(s):  
Tomy Kallarackal
Keyword(s):  
European Economic Community ◽  
Value Added ◽  
Tax Credit ◽  
Value Added Tax ◽  
Excise Duty ◽  
Goods And Services ◽  
The World ◽  
The Difference ◽  
The Government ◽  
Insight Into

The Value Added Tax was first introduced in France in 1954. It was the resultant effort of France and members of the European Economic Community (E.E.C) during the 1950s aimed at the simplification of commodity taxes. Currently more than 130 nations in the world have adopted the VAT system. In the last decade alone over 50 nations have introduced VAT. This includes implementation in China and most recently the addition of Australia to the list of VAT nations. The world over, VAT is payable on both goods and services as they constitute a part of the national GDR Excise duty and sales taxes are merged into the singularity of VAT. No tax is levied on exports with full input tax credit made available. The scheme of taxation adopted by most nations is very simple. The seller of goods and the service provider charge tax on sales, avail input tax credit and pay the difference as VAT to the goVernment treasury. The compliance system in VAT nations is also very simple. There is very less interface between the tax collector and the tax payer. However there are provisions for heavy penalization of VAT defaulters. VAT is administered nationally and is also levied on imports.  

scholarly journals Population Genomic- and Phylogenomic-Enabled Advances to Increase Insight Into Pathogen Biology and Epidemiology

2021 ◽  
Vol 111 (1) ◽  
pp. 8-11
Author(s):  
Remco Stam ◽  
Pierre Gladieux ◽  
Boris A. Vinatzer ◽  
Erica M. Goss ◽  
Neha Potnis ◽  
...  
Keyword(s):  
Population Genetics ◽  
Population Genomics ◽  
Cost Effective ◽  
Whole Genome ◽  
Genome Data ◽  
Sequencing Technologies ◽  
Population Genomic ◽  
High Throughput Dna Sequencing ◽  
Opening Up ◽  
Insight Into

Population genetics has been a key discipline in phytopathology for many years. The recent rise in cost-effective, high-throughput DNA sequencing technologies, allows sequencing of dozens, if not hundreds of specimens, turning population genetics into population genomics and opening up new, exciting opportunities as described in this Focus Issue . Without the limitations of genetic markers and the availability of whole or near whole-genome data, population genomics can give new insights into the biology, evolution and adaptation, and dissemination patterns of plant-associated microbes.

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