scholarly journals Characterization of the population structure, drug resistance mechanisms and plasmids of the community-associated Enterobacter cloacae complex in China

2017 ◽  
Vol 73 (1) ◽  
pp. 66-76 ◽  
Author(s):  
Kai Zhou ◽  
Wei Yu ◽  
Xiaoli Cao ◽  
Ping Shen ◽  
Haifeng Lu ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Population Structure ◽  
Enterobacter Cloacae ◽  
Resistance Mechanisms ◽  
Enterobacter Cloacae Complex

scholarly journals Characterization of resistance mechanisms of Enterobacter cloacae Complex co-resistant to carbapenem and colistin

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shixing Liu ◽  
Renchi Fang ◽  
Ying Zhang ◽  
Lijiang Chen ◽  
Na Huang ◽  
...  
Keyword(s):  
Lipid A ◽  
Efflux Pump ◽  
Resistance Mechanism ◽  
Carbapenem Resistance ◽  
Enterobacter Cloacae ◽  
Resistance Mechanisms ◽  
Colistin Resistance ◽  
Carbapenem Resistant ◽  
Horizontal Spread

Abstract Background The emergence of carbapenem-resistant and colistin-resistant ECC pose a huge challenge to infection control. The purpose of this study was to clarify the mechanism of the carbapenems and colistin co-resistance in Enterobacter cloacae Complex (ECC) strains. Results This study showed that the mechanisms of carbapenem resistance in this study are: 1. Generating carbapenemase (7 of 19); 2. The production of AmpC or ESBLs combined with decreased expression of out membrane protein (12 of 19). hsp60 sequence analysis suggested 10 of 19 the strains belong to colistin hetero-resistant clusters and the mechanism of colistin resistance is increasing expression of acrA in the efflux pump AcrAB-TolC alone (18 of 19) or accompanied by a decrease of affinity between colistin and outer membrane caused by the modification of lipid A (14 of 19). Moreover, an ECC strain co-harboring plasmid-mediated mcr-4.3 and blaNDM-1 has been found. Conclusions This study suggested that there is no overlap between the resistance mechanism of co-resistant ECC strains to carbapenem and colistin. However, the emergence of strain co-harboring plasmid-mediated resistance genes indicated that ECC is a potential carrier for the horizontal spread of carbapenems and colistin resistance.

Characterization of NDM-1- and CMH-3-producing Enterobacter cloacae complex ST932 in a patient transferred from Ukraine to Spain

2020 ◽  
Vol 38 (7) ◽  
pp. 327-330
Author(s):  
Inmaculada López-Hernández ◽  
Aurora García Barrionuevo ◽  
Paula Díaz de Alba ◽  
Encarnación Clavijo ◽  
Alvaro Pascual
Keyword(s):  
Enterobacter Cloacae ◽  
Enterobacter Cloacae Complex

scholarly journals Genomic Study of blaIMI-Positive Enterobacter cloacae Complex in Singapore over a Five-Year Study Period

2020 ◽  
Vol 64 (8) ◽  
Author(s):  
Sophie Octavia ◽  
Tse Hsien Koh ◽  
Oon Tek Ng ◽  
Kalisvar Marimuthu ◽  
Indumathi Venkatachalam ◽  
...  
Keyword(s):  
Enterobacter Cloacae ◽  
Mobile Elements ◽  
Content Type ◽  
Genomic Study ◽  
Genomic Characterization ◽  
Enterobacter Cloacae Complex

ABSTRACT The blaIMI gene is rarely detected outside the Enterobacter genus. Genomic characterization of 87 blaIMI-positive Enterobacter cloacae complex members revealed that the largest phylogenomic clade was made up of E. cloacae subsp. cloacae (71.3%), followed by the newly described species E. bugandensis (13.8%), E. sichuanensis (10.3%), and E. roggenkampii (4.6%). IMI-1 was the predominant carbapenemase variant (86/87, 98.9%). All the blaIMI genes were associated with chromosomally integrated Xer-dependent integrative mobile elements (IMEXs), with two new variants detected.

scholarly journals Genomic Characterization of VIM and MCR Co-Producers: The First Two Clinical Cases, in Italy

Diagnostics ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 79
Author(s):  
Vittoria Mattioni Marchetti ◽  
Ibrahim Bitar ◽  
Mario Sarti ◽  
Elena Fogato ◽  
Erika Scaltriti ◽  
...  
Keyword(s):  
Enterobacter Cloacae ◽  
Sequencing Technology ◽  
Genomic Features ◽  
Long Reads ◽  
Clinical Resistance ◽  
Genomic Characterization ◽  
Enterobacteriaceae Infections ◽  
Enterobacter Cloacae Complex ◽  
Conjugation Experiment

Background: the co-production of carbapenemases and mcr-genes represents a worrisome event in the treatment of Enterobacteriaceae infections. The aim of the study was to characterize the genomic features of two clinical Enterobacter cloacae complex (ECC) isolates, co-producing VIM and MCR enzymes, in Italy. Methods: species identification and antibiotic susceptibility profiling were performed using MALDI-TOF and broth microdilution methods, respectively. Transferability of the blaVIM- and mcr- type genes was verified through conjugation experiment. Extracted DNA was sequenced using long reads sequencing technology on the Sequel I platform (PacBio). Results: the first isolate showed clinical resistance against ertapenem yet was colistin susceptible (EUCAST 2020 breakpoints). The mcr-9.2 gene was harbored on a conjugative IncHI2 plasmid, while the blaVIM-1 determinant was harbored on a conjugative IncN plasmid. The second isolate, resistant to both carbapenems and colistin, harbored: mcr-9 gene and its two component regulatory genes for increased expression on the chromosome, mcr-4.3 on non-conjugative (yet co-transferable) ColE plasmid, and blaVIM-1 on a non-conjugative IncA plasmid. Conclusions: to our knowledge, this is the first report of co-production of VIM and MCR in ECC isolates in Italy.

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