scholarly journals High genetic diversity of nitrofurantoin- or mecillinam-resistant Escherichia coli indicates low propensity for clonal spread

2013 ◽  
Vol 68 (9) ◽  
pp. 1974-1977 ◽  
Author(s):  
Hanna O. Poulsen ◽  
Anders Johansson ◽  
Susanne Granholm ◽  
Gunnar Kahlmeter ◽  
Martin Sundqvist
Keyword(s):  
Escherichia Coli ◽  
Genetic Diversity ◽  
High Genetic Diversity ◽  
Clonal Spread

scholarly journals Transmission of IncN Plasmids Carrying blaCTX-M-1 between Commensal Escherichia coli in Pigs and Farm Workers

2009 ◽  
Vol 53 (4) ◽  
pp. 1709-1711 ◽  
Author(s):  
Arshnee Moodley ◽  
Luca Guardabassi
Keyword(s):  
Escherichia Coli ◽  
Genetic Diversity ◽  
Farm Workers ◽  
High Genetic Diversity ◽  
Air Samples ◽  
E Coli ◽  
Pig Farms ◽  
Cephalosporin Resistance ◽  
Human Animal

ABSTRACT CTX-M-1-producing Escherichia coli were isolated from 56 pigs, three farm personnel, two manure samples, and two air samples from two Danish pig farms where an association between prophylactic ceftiofur use and the occurrence of cephalosporin resistance was previously demonstrated. Human, animal, and environmental strains displayed high genetic diversity but harbored indistinguishable or closely related IncN plasmids carrying bla CTX-M-1, indicating that IncN plasmids mediating cephalosporin resistance were transmitted between pigs and farm workers across multiple E. coli lineages.

High genetic diversity of extended-spectrum β-lactamases producing Escherichia coli in feces of horses

2018 ◽  
Vol 219 ◽  
pp. 117-122 ◽  
Author(s):  
Syndia Sadikalay ◽  
Yann Reynaud ◽  
Stéphanie Guyomard-Rabenirina ◽  
Mélanie Falord ◽  
Célia Ducat ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Diversity ◽  
High Genetic Diversity ◽  
Extended Spectrum

scholarly journals Genetic heterogeneity of Escherichia coli isolated from pasteurized milk in State of Paraná, Brazil

2014 ◽  
Vol 50 (2) ◽  
pp. 337-343 ◽  
Author(s):  
Karine Oltramari ◽  
Rosilene Fressati Cardoso ◽  
Eliana Valéria Patussi ◽  
Adolfo Carlos Barreto Santos ◽  
Jane Martha Graton Mikcha
Keyword(s):  
Escherichia Coli ◽  
Genetic Diversity ◽  
Diarrheal Disease ◽  
Food Contamination ◽  
Economic Losses ◽  
High Morbidity ◽  
High Genetic Diversity ◽  
Pasteurized Milk ◽  
E Coli ◽  
Northwestern Region

Food contamination caused by enteric pathogens is a major cause of diarrheal disease worldwide, resulting in high morbidity and mortality and significant economic losses. Bacteria are important agents of foodborne diseases, particularly diarrheagenic Escherichia coli. The present study assessed the genetic diversity and antimicrobial resistance of E. coli isolates from pasteurized milk processed in 21 dairies in northwestern State of Parana, Brazil. The 95 E. coli isolates were subjected to antimicrobial susceptibility testing according to the recommendations of the Clinical and Laboratory Standards Institute and assessed genotypically by Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR). The highest rate of resistance was observed for cephalothin (55.78%). ERIC-PCR revealed high genetic diversity, clustering the 95 bacterial isolates into 90 different genotypic patterns. These results showed a heterogeneous population of E. coli in milk samples produced in the northwestern region of Paraná and the need for good manufacturing practices throughout the processing of pasteurized milk to reduce the risk of foodborne illnesses.

scholarly journals Insights into the genome architecture and evolution of Shiga toxin encoding bacteriophages of Escherichia coli

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Graça Pinto ◽  
Marta Sampaio ◽  
Oscar Dias ◽  
Carina Almeida ◽  
Joana Azeredo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Diversity ◽  
Shiga Toxin ◽  
Genetic Material ◽  
High Genetic Diversity ◽  
Vast Number ◽  
Coding Regions ◽  
Regulatory Systems ◽  
Geographical Regions ◽  
Lytic System

Abstract Background A total of 179 Shiga toxin-producing Escherichia coli (STEC) complete genomes were analyzed in terms of serotypes, prophage coding regions, and stx gene variants and their distribution. We further examined the genetic diversity of Stx-converting phage genomes (Stx phages), focusing on the lysis-lysogeny decision and lytic cassettes. Results We show that most STEC isolates belong to non-O157 serotypes (73 %), regardless the sources and geographical regions. While the majority of STEC genomes contain a single stx gene (61 %), strains containing two (35 %), three (3 %) and four (1 %) stx genes were also found, being stx2 the most prevalent gene variant. Their location is exclusively found in intact prophage regions, indicating that they are phage-borne. We further demonstrate that Stx phages can be grouped into four clusters (A, B, C and D), three subclusters (A1, A2 and A3) and one singleton, based on their shared gene content. This cluster distribution is in good agreement with their predicted virion morphologies. Stx phage genomes are highly diverse with a vast number of 1,838 gene phamilies (phams) of related sequences (of which 677 are orphams i.e. unique genes) and, although having high mosaicism, they are generally organized into three major transcripts. While the mechanisms that guide lysis–lysogeny decision are complex, there is a strong selective pressure to maintain the stx genes location close to the lytic cassette composed of predicted SAR-endolysin and pin-holin lytic proteins. The evolution of STEC Stx phages seems to be strongly related to acquiring genetic material, probably from horizontal gene transfer events. Conclusions This work provides novel insights on the genetic structure of Stx phages, showing a high genetic diversity throughout the genomes, where the various lysis-lysogeny regulatory systems are in contrast with an uncommon, but conserved, lytic system always adjacent to stx genes.

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