scholarly journals Immune cell-endothelial cell interactions in the bovine corpus luteum

2006 ◽  
Vol 46 (6) ◽  
pp. 1055-1059 ◽  
Author(s):  
D. H. Townson
Keyword(s):  
Endothelial Cell ◽  
Corpus Luteum ◽  
Immune Cell ◽  
Cell Interactions ◽  
Bovine Corpus Luteum

scholarly journals KIT receptor-positive cells in the bovine corpus luteum are primarily theca-derived small luteal cells

Reproduction ◽  
2007 ◽  
Vol 134 (4) ◽  
pp. 625-634 ◽  
Author(s):  
Katharina Spanel-Borowski ◽  
Kristina Sass ◽  
Sabine Löffler ◽  
Elke Brylla ◽  
Michiharu Sakurai ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Corpus Luteum ◽  
Double Staining ◽  
Double Immunofluorescence ◽  
Specific Enzyme ◽  
Thecal Cell ◽  
Luteal Cells ◽  
Bovine Corpus Luteum ◽  
Kit Receptor ◽  
Capillary Bed

The tyrosine kinase KIT receptor, the protooncogene CD117, plays a key role in growth and maturation of oocytes and follicles. Relevant data are sparse for the corpus luteum (CL). We first confirmed the presence ofKITmRNA and KIT protein in bovine CL homogenates. We then localized KIT-positive (KIT+) cells in CL sections by immunohistochemistry. At the CL stage of early development, the former theca transforming into capsule/septa showed a strong band-like KIT+ immunoresponse. In addition, CD45+ leukocytes in septa included subpopulations of CD45+/KIT+ and CD14+/KIT+ leukocytes as validated by double immunofluorescence localization. At the early secretory stage, KIT+ cells appeared within the septa/capsule region and in the periphery of the CL parenchyma, there forming a complex network. This was separate from the capillary bed as determined by double staining for CD117 and FVIII-related endothelial cell antigen (FVIIIr). The KIT+ network coincided with cells positive for cytochrome P450 17α-hydroxylase, a thecal cell-specific enzyme. The late secretory stage was defined by an advanced manifestation of the KIT+ network in the CL periphery. At the stage of regression, the KIT+ network was absent. The CL of pregnancy expressed high levels ofKITmRNA and KIT protein uniformly throughout pregnancy. The KIT+ immunolocalization revealed small fibroblast-like cells, luteal cells with granules, and clusters of large luteal cells with staining of the cell membrane. We conclude that a majority of KIT+ cells in the bovine CL are primarily theca-derived small luteal cells, and that a minority represent KIT+ leukocytes, in some cases KIT+ monocytes.

Lectin binding patterns in two cultured endothelial cell types derived from bovine corpus luteum

1996 ◽  
Vol 105 (2) ◽  
pp. 129-137 ◽  
Author(s):  
Gudrun Herrman ◽  
Hannah Missfelder ◽  
Katharina Spanel-Borowski
Keyword(s):  
Endothelial Cell ◽  
Corpus Luteum ◽  
Lectin Binding ◽  
Cell Types ◽  
Bovine Corpus Luteum
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