Lectin binding patterns in two cultured endothelial cell types derived from bovine corpus luteum

1996 ◽  
Vol 105 (2) ◽  
pp. 129-137 ◽  
Author(s):  
Gudrun Herrman ◽  
Hannah Missfelder ◽  
Katharina Spanel-Borowski
Keyword(s):  
Endothelial Cell ◽  
Corpus Luteum ◽  
Lectin Binding ◽  
Cell Types ◽  
Bovine Corpus Luteum

scholarly journals TAMANHO DA AMOSTRA PARA ESTUDO DA PROPORÇÃO VOLUMÉTRICA DOS CONSTITUINTES DO CORPO LÚTEO BOVINO

2002 ◽  
Vol 7 (2) ◽  
Author(s):  
M. M. NEVES ◽  
A. P. MARQUES JR.
Keyword(s):  
Endothelial Cells ◽  
Soybean Oil ◽  
Corpus Luteum ◽  
Cell Types ◽  
Luteal Cell ◽  
Cell Nuclei ◽  
Cell Cytoplasm ◽  
Bovine Corpus Luteum

O objetivo deste estudo foi estabelecer o número de campos histológicos necessários para quantificar os componentes celulares do corpo lúteo bovino. Para os parâmetros citoplasma das células luteínicas, núcleo das células luteínicas e fibroblasto são necessários 30 campos, enquanto que para células endoteliais e pericito são necessários 35, podendo-se padronizar a técnica em 35 campos e 875 pontos por corpo lúteo. Soybean oil replaced by acidulated soapstock in broiler diets Abstract The purpose of this study was to determine the number of fields necessary for the quantification of the cell types of the bovine corpus luteum. A total of 30 fields is necessary for the quantification of the luteal cell cytoplasm, luteal cell nuclei and fibroblast, and 35 fields for the quantification of endothelial cells and pericycles. For the analysis of both parameters is recommended the study of 35 fields and 875 points per corpus luteum.

scholarly journals KIT receptor-positive cells in the bovine corpus luteum are primarily theca-derived small luteal cells

Reproduction ◽  
2007 ◽  
Vol 134 (4) ◽  
pp. 625-634 ◽  
Author(s):  
Katharina Spanel-Borowski ◽  
Kristina Sass ◽  
Sabine Löffler ◽  
Elke Brylla ◽  
Michiharu Sakurai ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Corpus Luteum ◽  
Double Staining ◽  
Double Immunofluorescence ◽  
Specific Enzyme ◽  
Thecal Cell ◽  
Luteal Cells ◽  
Bovine Corpus Luteum ◽  
Kit Receptor ◽  
Capillary Bed

The tyrosine kinase KIT receptor, the protooncogene CD117, plays a key role in growth and maturation of oocytes and follicles. Relevant data are sparse for the corpus luteum (CL). We first confirmed the presence ofKITmRNA and KIT protein in bovine CL homogenates. We then localized KIT-positive (KIT+) cells in CL sections by immunohistochemistry. At the CL stage of early development, the former theca transforming into capsule/septa showed a strong band-like KIT+ immunoresponse. In addition, CD45+ leukocytes in septa included subpopulations of CD45+/KIT+ and CD14+/KIT+ leukocytes as validated by double immunofluorescence localization. At the early secretory stage, KIT+ cells appeared within the septa/capsule region and in the periphery of the CL parenchyma, there forming a complex network. This was separate from the capillary bed as determined by double staining for CD117 and FVIII-related endothelial cell antigen (FVIIIr). The KIT+ network coincided with cells positive for cytochrome P450 17α-hydroxylase, a thecal cell-specific enzyme. The late secretory stage was defined by an advanced manifestation of the KIT+ network in the CL periphery. At the stage of regression, the KIT+ network was absent. The CL of pregnancy expressed high levels ofKITmRNA and KIT protein uniformly throughout pregnancy. The KIT+ immunolocalization revealed small fibroblast-like cells, luteal cells with granules, and clusters of large luteal cells with staining of the cell membrane. We conclude that a majority of KIT+ cells in the bovine CL are primarily theca-derived small luteal cells, and that a minority represent KIT+ leukocytes, in some cases KIT+ monocytes.

Sign in / Sign up

Export Citation Format

Share Document