scholarly journals KIT receptor-positive cells in the bovine corpus luteum are primarily theca-derived small luteal cells

Reproduction ◽  
2007 ◽  
Vol 134 (4) ◽  
pp. 625-634 ◽  
Author(s):  
Katharina Spanel-Borowski ◽  
Kristina Sass ◽  
Sabine Löffler ◽  
Elke Brylla ◽  
Michiharu Sakurai ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Corpus Luteum ◽  
Double Staining ◽  
Double Immunofluorescence ◽  
Specific Enzyme ◽  
Thecal Cell ◽  
Luteal Cells ◽  
Bovine Corpus Luteum ◽  
Kit Receptor ◽  
Capillary Bed

The tyrosine kinase KIT receptor, the protooncogene CD117, plays a key role in growth and maturation of oocytes and follicles. Relevant data are sparse for the corpus luteum (CL). We first confirmed the presence ofKITmRNA and KIT protein in bovine CL homogenates. We then localized KIT-positive (KIT+) cells in CL sections by immunohistochemistry. At the CL stage of early development, the former theca transforming into capsule/septa showed a strong band-like KIT+ immunoresponse. In addition, CD45+ leukocytes in septa included subpopulations of CD45+/KIT+ and CD14+/KIT+ leukocytes as validated by double immunofluorescence localization. At the early secretory stage, KIT+ cells appeared within the septa/capsule region and in the periphery of the CL parenchyma, there forming a complex network. This was separate from the capillary bed as determined by double staining for CD117 and FVIII-related endothelial cell antigen (FVIIIr). The KIT+ network coincided with cells positive for cytochrome P450 17α-hydroxylase, a thecal cell-specific enzyme. The late secretory stage was defined by an advanced manifestation of the KIT+ network in the CL periphery. At the stage of regression, the KIT+ network was absent. The CL of pregnancy expressed high levels ofKITmRNA and KIT protein uniformly throughout pregnancy. The KIT+ immunolocalization revealed small fibroblast-like cells, luteal cells with granules, and clusters of large luteal cells with staining of the cell membrane. We conclude that a majority of KIT+ cells in the bovine CL are primarily theca-derived small luteal cells, and that a minority represent KIT+ leukocytes, in some cases KIT+ monocytes.

Influence of Nitric Oxide on the Secretory Function of the Bovine Corpus Luteum: Dependence on Cell Composition and Cell-to-Cell Communication

2003 ◽  
Vol 228 (6) ◽  
pp. 741-748 ◽  
Author(s):  
Jerzy J. Jaroszewski ◽  
Dariusz J. Skarzynski ◽  
Robert M. Blair ◽  
William Hansel
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Corpus Luteum ◽  
Cell Contact ◽  
Secretory Function ◽  
No Donor ◽  
Luteal Cells ◽  
Cell Composition ◽  
Bovine Corpus Luteum ◽  
Nos Inhibitor

The objective of the present study was to investigate the role of cell-to-cell contact in the influence of nitric oxide (NO) on the secretory function of the bovine corpus luteum (CL). In Experiment 1, separate small luteal cells (SLC) or large (LLC) luteal cells were perfused with 100 μ M spermineNONOate, a NO donor, or with 100 μ M Nω-nitro-L-arginine methyl ester (L-NAME), a NO synthase (NOS) inhibitor; in Experiment 2, a mixture of LLC and SLC and endothelial cells was cultured and incubated with spermineNONOate or L-NAME; in Experiment 3, spermineNONOate was perfused into the CL (100 mg/4 hr) by a microdialysis system in vivo. Perfusion of isolated SLC and LLC with the NO donor or NOS inhibitor (Experiment 1) did not affect ( P > 0.05) secretion of progesterone (P4) or oxytocin (OT). L-NAME perfusion increased ( P < 0.05) leukotriene C4 (LTC4) secretion by both SLC and LLC cells. Treatment of mixtures of luteal cells with an NO donor (Experiment 2) significantly decreased ( P < 0.001) secretion of P4 and OT and increased ( P < 0.001) production of prostaglandin F2α (PGF2α) and LTC4. L-NAME stimulated ( P < 0.001) P4 secretion, but did not influence ( P > 0.05) OT, PGF2α or LTC4 production. Intraluteal administration (Experiment 3) of spermineNONOate increased ( P < 0.001) LTC4 and PGF2α, decreased OT, but did not change P4 levels in perfusate samples. These data indicate that cell-to-cell contact and cell composition play important roles in the response of bovine CL to treatment with NO donors or NOS inhibitors, and that paracrine mechanisms are required for the full secretory response of the CL in NO action. Endothelial cells appear to be required for the full secretory response of the CL to NO.

scholarly journals Control of Steroidogenesis in Small and Large Bovine Luteal. Cells

1987 ◽  
Vol 40 (3) ◽  
pp. 331 ◽  
Author(s):  
William Hansel ◽  
Hector W Alila ◽  
Joseph P Dowd ◽  
Xiangzhong Yang
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Corpus Luteum ◽  
Luteal Cell ◽  
Lipoxygenase Pathway ◽  
Luteal Cells ◽  
Kinase C ◽  
Bovine Corpus Luteum ◽  
Progesterone Synthesis

Evidence was cited to show that: (1) prostacyclin (PGI2) plays a luteotrophic role in the bovine corpus luteum and that products of the lipoxygenase pathway of arachidonic acid metabolism, especially 5-hydroxyeicosatetraenoic acid play luteolytic roles; (2) oxytocin of luteal cell origin plays a role in development, and possibly in regression, of the bovine corpus luteum; and (3) luteal cells arise from two sources; the characteristic small luteal cells at all stages of the o~strous cycle and pregnancy are of theca cell origin; the large cells are of granulosa cell origin early in the cycle, but a population of theca-derived large cells appears later in the cycle. Results of in vitro studies with total dispersed cells and essentially pure preparations of large and small luteal cells indicate that : (1) the recently described Ca2+ -polyphosphoinositol-protein kinase C second messenger system is involved in progesterone synthesis in the bovine corpus luteum; (2) activation of protein kinase C is stimulatory to progesterone synthesis in the small luteal cells; (3) activation of protein kinase C has no effect on progesterone synthesis in the large luteal cells; and (4) protein kinase C exerts its luteotrophic effect in total cell preparations, in part at least, by stimulating the production of prostacyclin. The protein kinase C system may cause down regulation of LH receptors in the large cells.

β-Carotene and protein complex found in microsomal membranes of bovine corpora lutea

1996 ◽  
Vol 1996 ◽  
pp. 69-69
Author(s):  
AJ Holt ◽  
RG Rodway ◽  
JBC Findlay ◽  
HS Sands ◽  
DN Batchelder
Keyword(s):  
Corpus Luteum ◽  
High Performance ◽  
Gel Filtration ◽  
Biological Action ◽  
Corpora Lutea ◽  
Luteal Cells ◽  
Bovine Corpus Luteum ◽  
Microsomal Membranes ◽  
Ruminant Animals ◽  
Β Carotene

The role of β-carotene in the fertility of ruminant animals has long been acknowledged (Friesecke, 1978). Initially this was thought to be due to its action as a vitamin A precursor but recently β-carotene itself has been considered responsible for improving reproductive performance (Hurley & Doane, 1989). The mechanism by which β-carotcne acts is unclear, but as its concentration in the corpus luteum was typically found to be 70μg per gram of tissue, its biological action is probably exerted in this area.β-Carotene has been studied in the bovine corpus luteum using Raman spectroscopy, high performance liquid chromatography (HPLC) and gel filtration chromatography. The structure of β-carotene produces a characteristic Raman spectrum and by utilising an imaging technique, photographs of isolated luteal cells were obtained indicating the regions of β-carotene within them. Differential centrifugation was used to obtain pure subcellular fractions of luteal cells.

Lectin binding patterns in two cultured endothelial cell types derived from bovine corpus luteum

1996 ◽  
Vol 105 (2) ◽  
pp. 129-137 ◽  
Author(s):  
Gudrun Herrman ◽  
Hannah Missfelder ◽  
Katharina Spanel-Borowski
Keyword(s):  
Endothelial Cell ◽  
Corpus Luteum ◽  
Lectin Binding ◽  
Cell Types ◽  
Bovine Corpus Luteum

scholarly journals Expression and regulation of secreted phosphoprotein 1 in the bovine corpus luteum and effects on T lymphocyte chemotaxis

Reproduction ◽  
2013 ◽  
Vol 146 (6) ◽  
pp. 527-537 ◽  
Author(s):  
Daniel H Poole ◽  
Kalidou Ndiaye ◽  
Joy L Pate
Keyword(s):  
Corpus Luteum ◽  
Estrous Cycle ◽  
Cell Function ◽  
Luteal Cell ◽  
Cellular Interactions ◽  
Western Blots ◽  
Luteal Regression ◽  
Luteal Cells ◽  
Bovine Corpus Luteum ◽  
Secreted Phosphoprotein 1

Secreted phosphoprotein 1 (SPP1) in the bovine corpus luteum (CL) regulates cell function during the transitional periods of luteinization and luteal regression. The objectives were to i) characterize SPP1 expression in the CL throughout the estrous cycle, ii) determine factors that regulate SPP1 expression in luteal cells, and iii) examine the role of SPP1 in lymphocyte chemotaxis, proliferation, and function.SPP1mRNA was greater in fully functional (d10) CL and late cycle (d18) CL compared with developing (d4) CL. Additionally,SPP1mRNA increased within 1 h and remained elevated 4 and 8 h following induction of luteolysis with prostaglandin (PG)F2α. Expression of the SPP1 receptor, β3integrin, was not different throughout the estrous cycle but decreased following induction of luteolysis. Expression ofCD44increased during the estrous cycle but did not change during luteal regression. In cultured luteal cells,SPP1mRNA was upregulated by PGF2αand/or tumor necrosis factor α. Western blots revealed the presence of both full-length SPP1 and multiple cleavage products in cultured luteal cells and luteal tissue. Depletion of endogenous SPP1 did not hinder luteal cell-induced lymphocyte proliferation or lymphocyte phenotype but did inhibit lymphocyte migration toward luteal cells. Based on these data, it is concluded that SPP1 is initially activated to establish and maintain cellular interactions between steroidogenic and nonsteroidogenic cells during the development of the CL. Upon induction of luteolysis, SPP1 serves as a signaling molecule to recruit or activate immune cells to facilitate luteal regression and tissue degradation.

β-Carotene and protein complex found in microsomal membranes of bovine corpora lutea

1996 ◽  
Vol 1996 ◽  
pp. 69-69
Author(s):  
AJ Holt ◽  
RG Rodway ◽  
JBC Findlay ◽  
HS Sands ◽  
DN Batchelder
Keyword(s):  
Corpus Luteum ◽  
High Performance ◽  
Gel Filtration ◽  
Biological Action ◽  
Corpora Lutea ◽  
Luteal Cells ◽  
Bovine Corpus Luteum ◽  
Microsomal Membranes ◽  
Ruminant Animals ◽  
Β Carotene

The role of β-carotene in the fertility of ruminant animals has long been acknowledged (Friesecke, 1978). Initially this was thought to be due to its action as a vitamin A precursor but recently β-carotene itself has been considered responsible for improving reproductive performance (Hurley & Doane, 1989). The mechanism by which β-carotcne acts is unclear, but as its concentration in the corpus luteum was typically found to be 70μg per gram of tissue, its biological action is probably exerted in this area.β-Carotene has been studied in the bovine corpus luteum using Raman spectroscopy, high performance liquid chromatography (HPLC) and gel filtration chromatography. The structure of β-carotene produces a characteristic Raman spectrum and by utilising an imaging technique, photographs of isolated luteal cells were obtained indicating the regions of β-carotene within them. Differential centrifugation was used to obtain pure subcellular fractions of luteal cells.

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