scholarly journals Cell-lineage specificity of primary cilia during postnatal epididymal development

2018 ◽  
Vol 33 (10) ◽  
pp. 1829-1838 ◽  
Author(s):  
Agathe Bernet ◽  
Alexandre Bastien ◽  
Denis Soulet ◽  
Olivia Jerczynski ◽  
Christian Roy ◽  
...  
Keyword(s):  
Primary Cilia ◽  
Cell Lineage ◽  
Lineage Specificity

scholarly journals Cell-lineage specificity of primary cilia during epididymis post-natal development

2018 ◽  
Author(s):  
Agathe Bernet ◽  
Alexandre Bastien ◽  
Denis Soulet ◽  
Olivia Jerczynski ◽  
Christian Roy ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Hedgehog Signaling ◽  
Primary Cilia ◽  
Cell Lineage ◽  
Basal Cells ◽  
Myoid Cells ◽  
Apical Pole ◽  
Lineage Specificity ◽  
Spatio Temporal ◽  
Temporal Localization

AbstractPrimary cilia are sensory organelles that orchestrate major signaling pathways during organ development and homeostasis. By using a double Arl13b/mCherry-Cetn2/GFP transgenic mouse model, we characterized the spatio-temporal localization of primary cilia in the epididymis, from birth to adulthood. We report here a constitutive localisation of primary cilia in peritubular myoid cells and a dynamic profiling in differentiated epithelial cells throughout post-natal development. While primary cilia are present at the apical pole of the undifferentiated epithelial cells from birth to puberty, they are absent from the apical pole of the epithelium in adults, where they appear exclusively associated with cytokeratin 5-positive basal cells. Exogenous labeling of primary cilia marker Arl13b and IFT88 confirmed the cell lineage specific localization of primary cilia in basal cells and myoid cells in human epididymides. From whole epididymis tissues and serum-free cultures of DC2 murine epididymal principal cell lines we determined that primary cilia from the epididymis are associated with the polycystic kidney disease-related proteins polycystin 1 (PC1) and polycystin 2 (PC2), and Gli3 Hedgehog signaling transcription factor. Thus, our findings unveil the existence of primary cilia sensory organelles, which have the potential to mediate mechano/ chemo-signaling events in the epididymis.

scholarly journals The cell cycle state defines TACC3 as a regulator gene in glioblastoma

2020 ◽  
Author(s):  
Holly Briggs ◽  
Euan S. Polson ◽  
Bronwyn K. Irving ◽  
Alexandre Zougman ◽  
Ryan K. Mathew ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Gene Networks ◽  
Coiled Coil ◽  
Cell Lineage ◽  
Progression Free Survival ◽  
Low Grade ◽  
Loss Of Function ◽  
Feature Maps ◽  
Clinical Databases ◽  
Lineage Specificity

AbstractOverexpression and mitosis-promoting roles of Transforming acidic coiled-coil containing protein 3 (TACC3) are well-established in many cancers, including glioblastoma (GBM). However, the effector gene networks downstream of TACC3 remain poorly defined, partly due to an incomplete understanding of TACC3 cell lineage specificity and its dynamic role during the cell cycle. Here, we use a patient-derived GBM model to report that TACC3 predominantly resides in the GBM cell cytoplasm, while engaging in gene regulation temporally as defined by the cell cycle state. TACC3 loss-of-function, cell cycle stage-specific transcriptomics, and unsupervised self-organizing feature maps revealed pathways (including Hedgehog signalling) and individual genes (including HOTAIR) that exhibited anticorrelated expression phenotypes across interphase and mitosis. Furthermore, this approach identified a set of 22 TACC3-dependent transcripts in publicly-available clinical databases that predicted poor overall and progression-free survival in 162 GBM and 514 low-grade glioma patient samples. These findings uncover TACC3-dependent genes as a function of TACC3 cell cycle oscillation, which is important for TACC3-targeting strategies, and for predicting poor outcomes in brain cancer patients.

scholarly journals Caspase-3 Gene Knockout Defines Cell Lineage Specificity for Programmed Cell Death Signaling in the Ovary*

Endocrinology ◽  
2001 ◽  
Vol 142 (6) ◽  
pp. 2468-2480 ◽  
Author(s):  
Tiina Matikainen ◽  
Gloria I. Perez ◽  
Timothy S. Zheng ◽  
Thomas R. Kluzak ◽  
Bo R. Rueda ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Caspase 3 ◽  
Gene Knockout ◽  
Cell Lineage ◽  
Lineage Specificity ◽  
Cell Death Signaling

scholarly journals Lymphoid tumors displaying rearrangements of both immunoglobulin and T cell receptor genes.

1985 ◽  
Vol 162 (3) ◽  
pp. 1015-1024 ◽  
Author(s):  
P G Pelicci ◽  
D M Knowles ◽  
R Dalla Favera
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Lineage ◽  
Surface Antigens ◽  
Cell Receptor ◽  
Cell Populations ◽  
Cell Surface Antigens ◽  
T Cell Receptor Genes ◽  
Lineage Specificity ◽  
Beta Gene

Ig and T beta gene rearrangements can be used as genetic markers of lineage and clonality in the study of B and T cell populations. We have addressed the issue of the respective B and T lineage specificity of these rearrangements by analyzing a panel of 63 lymphoid tumors representative of the various clinicopathologic categories of both B and T neoplasias. We report that approximately 10% of the cases tested displayed rearrangements of both Ig and T beta genes. Despite their dual genotypic pattern, these tumors retain a pure immunophenotype, i.e. they display either B or T cell lineage-restricted cell surface antigens. The implications of these findings for both normal and neoplastic lymphoid differentiation are discussed.

scholarly journals Cell lineage specificity in G-CSF receptor gene methylation

Leukemia ◽  
1999 ◽  
Vol 13 (4) ◽  
pp. 530-534 ◽  
Author(s):  
J Felgner ◽  
K Heidorn ◽  
D Körbächer ◽  
SO Frahm ◽  
R Parwaresch
Keyword(s):  
Receptor Gene ◽  
Cell Lineage ◽  
Gene Methylation ◽  
Lineage Specificity

scholarly journals Lineage specificity of primary cilia in the mouse embryo

2015 ◽  
Vol 17 (2) ◽  
pp. 113-122 ◽  
Author(s):  
Fiona K. Bangs ◽  
Nadine Schrode ◽  
Anna-Katerina Hadjantonakis ◽  
Kathryn V. Anderson
Keyword(s):  
Mouse Embryo ◽  
Primary Cilia ◽  
Lineage Specificity

scholarly journals Cell lineage specificity of chromatin configuration around the immunoglobulin heavy chain enhancer.

1988 ◽  
Vol 7 (8) ◽  
pp. 2393-2399 ◽  
Author(s):  
A. M. Ford ◽  
S. M. Watt ◽  
A. J. Furley ◽  
H. V. Molgaard ◽  
M. F. Greaves
Keyword(s):  
Heavy Chain ◽  
Cell Lineage ◽  
Immunoglobulin Heavy Chain ◽  
Lineage Specificity ◽  
Chromatin Configuration

scholarly journals Cell-lineage specificity and role of AP-1 in the prostate fibroblast androgen receptor cistrome

2017 ◽  
Vol 439 ◽  
pp. 261-272 ◽  
Author(s):  
Damien A. Leach ◽  
Vasilios Panagopoulos ◽  
Claire Nash ◽  
Charlotte Bevan ◽  
Axel A. Thomson ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Cell Lineage ◽  
Lineage Specificity
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