scholarly journals Lineage specificity of primary cilia in the mouse embryo

2015 ◽  
Vol 17 (2) ◽  
pp. 113-122 ◽  
Author(s):  
Fiona K. Bangs ◽  
Nadine Schrode ◽  
Anna-Katerina Hadjantonakis ◽  
Kathryn V. Anderson
Keyword(s):  
Mouse Embryo ◽  
Primary Cilia ◽  
Lineage Specificity

Type I collagen promotes primary cilia growth through down-regulating HDAC6-mediated autophagy in confluent mouse embryo fibroblast 3T3-L1 cells

2018 ◽  
Vol 125 (1) ◽  
pp. 8-14 ◽  
Author(s):  
Qian Xu ◽  
Weiwei Liu ◽  
Xiaoling Liu ◽  
Wuxiyar Otkur ◽  
Toshihiko Hayashi ◽  
...  
Keyword(s):  
Mouse Embryo ◽  
Primary Cilia ◽  
Type I Collagen ◽  
Type I ◽  
Mouse Embryo Fibroblast

scholarly journals Cell-lineage specificity of primary cilia during epididymis post-natal development

2018 ◽  
Author(s):  
Agathe Bernet ◽  
Alexandre Bastien ◽  
Denis Soulet ◽  
Olivia Jerczynski ◽  
Christian Roy ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Hedgehog Signaling ◽  
Primary Cilia ◽  
Cell Lineage ◽  
Basal Cells ◽  
Myoid Cells ◽  
Apical Pole ◽  
Lineage Specificity ◽  
Spatio Temporal ◽  
Temporal Localization

AbstractPrimary cilia are sensory organelles that orchestrate major signaling pathways during organ development and homeostasis. By using a double Arl13b/mCherry-Cetn2/GFP transgenic mouse model, we characterized the spatio-temporal localization of primary cilia in the epididymis, from birth to adulthood. We report here a constitutive localisation of primary cilia in peritubular myoid cells and a dynamic profiling in differentiated epithelial cells throughout post-natal development. While primary cilia are present at the apical pole of the undifferentiated epithelial cells from birth to puberty, they are absent from the apical pole of the epithelium in adults, where they appear exclusively associated with cytokeratin 5-positive basal cells. Exogenous labeling of primary cilia marker Arl13b and IFT88 confirmed the cell lineage specific localization of primary cilia in basal cells and myoid cells in human epididymides. From whole epididymis tissues and serum-free cultures of DC2 murine epididymal principal cell lines we determined that primary cilia from the epididymis are associated with the polycystic kidney disease-related proteins polycystin 1 (PC1) and polycystin 2 (PC2), and Gli3 Hedgehog signaling transcription factor. Thus, our findings unveil the existence of primary cilia sensory organelles, which have the potential to mediate mechano/ chemo-signaling events in the epididymis.

scholarly journals Cell-lineage specificity of primary cilia during postnatal epididymal development

2018 ◽  
Vol 33 (10) ◽  
pp. 1829-1838 ◽  
Author(s):  
Agathe Bernet ◽  
Alexandre Bastien ◽  
Denis Soulet ◽  
Olivia Jerczynski ◽  
Christian Roy ◽  
...  
Keyword(s):  
Primary Cilia ◽  
Cell Lineage ◽  
Lineage Specificity

scholarly journals Primary Cilia Are Not Required for Normal Canonical Wnt Signaling in the Mouse Embryo

PLoS ONE ◽  
2009 ◽  
Vol 4 (8) ◽  
pp. e6839 ◽  
Author(s):  
Polloneal Jymmiel R. Ocbina ◽  
Miquel Tuson ◽  
Kathryn V. Anderson
Keyword(s):  
Wnt Signaling ◽  
Mouse Embryo ◽  
Primary Cilia ◽  
Canonical Wnt Signaling ◽  
Canonical Wnt

Spontaneous Production of Type C Virus Particles in a Culture Derived from Rat Embryo Cells

1972 ◽  
Vol 30 ◽  
pp. 288-289
Author(s):  
Elizabeth S. Priori ◽  
T. Shigematsu ◽  
B. Myers ◽  
L. Dmochowski
Keyword(s):  
Virus Particle ◽  
Mouse Embryo ◽  
Calf Serum ◽  
Embryo Cell ◽  
Virus Particles ◽  
Extracellular Virus ◽  
Mouse Embryo Cell ◽  
Mature Virus Particle ◽  
Embryo Cells ◽  
Type C

Spontaneous release of type C virus particles in long-term cultures of mouse embryo cells as well as induction of similar particles in mouse embryo cell cultures with IUDR or BUDR have been reported. The presence of type C virus particles in cultures of normal rat embryos has not been reported.NB-1, a culture derived from embryos of a New Zealand Black (NB) rat (rats obtained from Mr. Samuel M. Poiley, N.C.I., Bethesda, Md.) and grown in McCoy's 5A medium supplemented with 20% fetal calf serum was passaged weekly. Extracellular virus particles similar to murine leukemia particles appeared in the 22nd subculture. General appearance of cells in passage 23 is shown in Fig. 1. Two budding figures and one immature type C virus particle may be seen in Fig. 2. The virus particles and budding were present in all further passages examined (currently passage 39). Various stages of budding are shown in Figs. 3a,b,c,d. Appearance of a mature virus particle is shown in Fig. 4.

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