scholarly journals THE CHROMOSOME OF BACILLUS SUBTILIS. I. THEORY OF MARKER FREQUENCY ANALYSIS

Genetics ◽  
1965 ◽  
Vol 52 (4) ◽  
pp. 747-757 ◽  
Author(s):  
Noboru Sueoka ◽  
Hiroshi Yoshikawa
Keyword(s):  
Bacillus Subtilis ◽  
Frequency Analysis ◽  
Marker Frequency

scholarly journals The order of replication of chromosomal markers inPseudomonas aeruginosastrain 1: I. Marker frequency analysis by transduction

1974 ◽  
Vol 23 (2) ◽  
pp. 145-153 ◽  
Author(s):  
R. J. Booker ◽  
J. S. Loutit
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Frequency Analysis ◽  
Relative Position ◽  
Circular Chromosome ◽  
Chromosome Map ◽  
Chromosomal Markers ◽  
Marker Frequency ◽  
Slow Growing

SUMMARYThe generalized transducing phage F116 has been used to prepare lysates from fast- and slow-growing cultures ofPseudomonas aeruginosastrain 1. These lysates have been used to transduce a number of auxotrophic markers to prototrophy and the ratios of the numbers of transductants obtained with each lysate have been determined. Since the markers are those which have been mapped by conjugation in previous studies it has been possible to compare the ratios obtained for each marker with the relative position of the marker on the chromosome map. If the assumption is made that there is only one circular chromosome inP. aeruginosastrain 1 it is possible to suggest a way in which two apparently unlinked segments might be joined together. It is also possible to suggest that the chromosome replicates sequentially in two directions from a fixed origin.

scholarly journals Effect of Miracil D on Marker Frequency Ratio and Cytotoxicity in Bacillus subtilis

1970 ◽  
Vol 102 (3) ◽  
pp. 835-842 ◽  
Author(s):  
C. W. Haidle ◽  
B. R. Brinkley ◽  
Manley Mandel
Keyword(s):  
Bacillus Subtilis ◽  
Frequency Ratio ◽  
Marker Frequency

scholarly journals THE RELATIVE NUMBERS OF DIFFERENT GENES IN EXPONENTIAL MICROBIAL CULTURES

Genetics ◽  
1974 ◽  
Vol 76 (3) ◽  
pp. 401-410
Author(s):  
P R Painter
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Genetic Mapping ◽  
Frequency Analysis ◽  
Dna Hybridization ◽  
Gene Frequencies ◽  
Microbial Cultures ◽  
Replication Time ◽  
Statistical Variations ◽  
Marker Frequency

ABSTRACT It is shown that the results of the marker frequency analysis of Sueoka and Yoshikawa (1965) can be derived as very good approximations from a model where the rigid assumptions of their analysis are relaxed to take into account statistical variations in the timing of cell events. It is further shown that the expression for the amount of DNA per cell can be approximated by an elementary exponential function of the growth rate, and this result facilitates genetic mapping by DNA hybridization techniques. An analysis of recent data on gene frequencies in Escherichia coli corroborates a model of symmetric, bidirectional chromosome replication with a replication time of approximately thirty minutes.

An expanded genomic map of Staphylococcus aureus

1971 ◽  
Vol 17 (9) ◽  
pp. 1239-1242 ◽  
Author(s):  
Robert A. Altenbern
Keyword(s):  
Staphylococcus Aureus ◽  
Frequency Analysis ◽  
Chromosomal Mapping ◽  
Chromosomal Replication ◽  
Genomic Map ◽  
Marker Frequency

A genomic map of 26 separate loci of Staphylococcus aureus has been derived by a combination of two methods of chromosomal mapping, by synchronous chromosomal replication and by marker frequency analysis.

Chromosomal mapping of Brucella abortus, strain 19

1973 ◽  
Vol 19 (1) ◽  
pp. 109-112 ◽  
Author(s):  
Robert A. Altenbern
Keyword(s):  
Frequency Analysis ◽  
Brucella Abortus ◽  
Chromosomal Mapping ◽  
Chromosomal Replication ◽  
Replication Time ◽  
Genomic Map ◽  
Marker Frequency ◽  
Duplication Time

A genomic map of Brucella abortus, strain 19, containing the relative locations of 19 genes has been derived by use of marker frequency analysis. Under the conditions used, the chromosomal replication time was approximately 60 min and the mass duplication time was 216 min.

Marker frequency analysis mapping of the Staphylococcus aureus chromosome

1971 ◽  
Vol 17 (7) ◽  
pp. 903-909 ◽  
Author(s):  
Robert A. Altenbern
Keyword(s):  
Staphylococcus Aureus ◽  
Frequency Analysis ◽  
Exponential Growth ◽  
Synthetic Medium ◽  
Mutation Induction ◽  
Resistance Locus ◽  
Chromosomal Replication ◽  
Genomic Map ◽  
Marker Frequency ◽  
Logarithmic Growth

Marker frequency analysis by mutation induction of several strains of Staphylococcus aureus has yielded a genomic map supporting the map derived by another technique. Gene orders of exponential cells obtained by using reference cells with completed chromosomes effected by chloramphenicol or by phenethanol treatment were identical, suggesting that these two agents halted chromosomal replication at a common terminus. In several strains, the pantothenate or acriflavine resistance locus changed its apparent chromosomal position during exponential growth. The data indicate that more than one chromosomal growing point occurred during logarithmic growth in non-synthetic medium.

Use of Antibody to aid Visualization of Protein at the Termini of Bacteriophage Ø29 DNA

1980 ◽  
Vol 38 ◽  
pp. 540-541
Author(s):  
Dwight Anderson ◽  
Charlene Peterson ◽  
Gursaran Notani ◽  
Bernard Reilly
Keyword(s):  
Electron Microscopy ◽  
Bacillus Subtilis ◽  
Dna Synthesis ◽  
Restriction Endonuclease ◽  
Protein Complex ◽  
Protein Product ◽  
Viral Dna ◽  
Small Proteins ◽  
Antibody Labeling ◽  
Subtilis Bacteriophage

The protein product of cistron 3 of Bacillus subtilis bacteriophage Ø29 is essential for viral DNA synthesis and is covalently bound to the 5’-termini of the Ø29 DNA. When the DNA-protein complex is cleaved with a restriction endonuclease, the protein is bound to the two terminal fragments. The 28,000 dalton protein can be visualized by electron microscopy as a small dot and often is seen only when two ends are in apposition as in multimers or in glutaraldehyde-fixed aggregates. We sought to improve the visibility of these small proteins by use of antibody labeling.

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