scholarly journals Dispersion Patterns and Sample Size Estimates for Egg Masses of Spotted Lanternfly (Hemiptera: Fulgoridae)

2020 ◽  
Vol 49 (6) ◽  
pp. 1462-1472
Author(s):  
J Keller ◽  
J Rost ◽  
K Hoover ◽  
J Urban ◽  
H Leach ◽  
...  

Abstract The spotted lanternfly, Lycorma delicatula (White), is a new invasive pest in the United States. To quantify spotted lanternfly population abundance, one must understand this pest’s dispersion pattern, that is, the spatial arrangement of individuals within a population. Spotted lanternflies overwinter in egg masses from late fall to May, making this life stage suitable for population assessments. We measured the dispersion pattern of egg masses at two types of sites: a suburban housing development, where we used individual trees as the sampling unit, and rural woodlots, where we used individual trees and also plots with 5.64 m radius as sampling units. Plots were the same size as those recommended for monitoring the gypsy moth, a well-studied pest with similar egg laying habit to the spotted lanternfly. Egg masses in both sampling units were counted up to a height of 3 m. With trees as the sampling unit, egg masses were aggregated in 12 of 20 rural sampling universes, randomly dispersed at 6, and completely absent at 2. Similar patterns were seen when using the 5.64-m radius rural sampling units and for suburban sampling universes. We calculated sample size requirements for a range of mean densities at a precision of 25 and 30%. Additionally, the vertical distribution of egg masses was characterized on the invasive tree of heaven [Ailanthus altissima (Mill.) Swingle], a preferred host for spotted lanternflies. For small trees, there was a positive relationship between number of egg masses in the bottom 3 m of the tree and the total count.

2021 ◽  
Author(s):  
Dalton C Ludwick ◽  
Layne B Leake ◽  
William R Morrison ◽  
Jesús R Lara ◽  
Mark S Hoddle ◽  
...  

Abstract Halyomorpha halys (Stål) is an invasive pest in the United States and other countries. In its native range, H. halys eggs are parasitized by a co-evolved parasitoid, Trissolcus japonicus (Ashmead). In the United States, T. japonicus, a classical biological control candidate, is being redistributed in many states where adventive populations exist. To establish if H. halys egg holding conditions affect T. japonicus foraging behavior or successful parasitism, naïve, female parasitoids from an adventive population were allowed to forage in laboratory bioassay arenas with either fresh or frozen (−20 or −80°C) egg masses, the latter held for five durations ranging from 1 h to 112 d. Parasitoid movements were recorded for 1 h. Thereafter, parasitoids were transferred with the same egg mass for 23 h. Additionally, female parasitoids from a quarantine colony were exposed to: 1) pairs of fresh egg masses and egg masses frozen at −40°C (>24 h) or 2) a single fresh egg mass or egg mass frozen at −40°C (<1 h). All exposed egg masses were held to assess progeny emergence. In the foraging bioassay, holding temperature and storage duration appeared to influence host-finding and host quality. Egg masses held at −80°C and fresh egg masses resulted in significantly greater levels of parasitism and progeny emergence compared with eggs held at −20°C. No differences were recorded between egg masses held at −40°C for ≤1 h and fresh egg masses. These results will help refine methods for preparation of egg masses for sentinel monitoring and parasitoid mass rearing protocols.


2019 ◽  
Vol 112 (4) ◽  
pp. 1997-2000 ◽  
Author(s):  
N F Quinn ◽  
E J Talamas ◽  
T C Leskey ◽  
J C Bergh

Abstract Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) is an invasive pest that has established in much of the United States. Adventive populations of an effective Asian egg parasitoid of H. halys, Trissolcus japonicus (Ashmead) (Hymenoptera: Scelionidae), have been detected in several states, including Virginia, and its geographic range is expanding. Documenting changes in its distribution and abundance have thus become key research priorities. For these specific purposes, surveillance of T. japonicus over large geographic areas using sentinel H. halys egg masses may not be optimally efficient, and examination of alternative sampling tactics is warranted. In 2016, sentinel H. halys egg masses were deployed as vertical transects in the canopy of female Ailanthus altissima (Mill.) Swingle (Sapindales: Simaroubaceae) in Virginia. A brief follow-up study in 2016 using yellow sticky traps deployed in the same trees yielded captures of T. japonicus, leading to a comparison of vertical transects of sentinel eggs and yellow sticky traps in 2017. Both methods yielded T. japonicus detections only in the middle and upper tree canopies, whereas other known H. halys parasitoids were detected in the lower, middle, or upper canopies. Based on this information, a method for deploying yellow sticky traps in the middle canopy of H. halys host trees was assessed in 2017, yielding T. japonicus captures. A comparison of estimated time inputs revealed that traps were more efficient than sentinel eggs in this regard. Results are discussed in relation to the utility of each sampling method to address specific questions about the range expansion and ecology of T. japonicus.


Author(s):  
James T. Vogt ◽  
David R. Coyle ◽  
David Jenkins ◽  
Chris Barnes ◽  
Christopher Crowe ◽  
...  

Abstract Callery pear (Pyrus calleryana Decne.) is rapidly spreading in the United States, gaining attention in the last two decades as a serious invasive pest. Recommended control methods include foliar, basal bark, cut stump, and hack-and-squirt application of herbicides, but there are few published studies with replicated data on efficacy. Four readily available herbicidal active ingredients and a combination of two active ingredients were tested for control efficacy against P. calleryana in old-field areas and loblolly pine (Pinus taeda L.) understory. Basal bark applications (triclopyr, triclopyr + aminopyralid), foliar applications (glyphosate, imazapyr), and a soil application (hexazinone) effectively killed P. calleryana with the exception of hexazinone at one site, where rainfall may not have been optimal. Foliar application of glyphosate provided the most consistent control. Our results demonstrate efficacy of registered herbicide formulations for P. calleryana control in two geographic locations and two habitat types. The need for development of integrated pest management programs for P. calleryana is discussed.


2019 ◽  
Vol 19 (4) ◽  
Author(s):  
Joshua M Milnes ◽  
Elizabeth H Beers

Abstract Trissolcus japonicus (Ashmead), an Asian parasitoid of Halyomorpha halys (Stål) (Hemiptera: Pentatomidae), was first detected in North America in 2014. Although testing in quarantine facilities as a candidate for classical biological control is ongoing, adventive populations have appeared in multiple sites in the United States, Canada, and Europe. Extensive laboratory testing of T. japonicus against other North American pentatomids and H. halys has revealed a higher rate of parasitism of H. halys, but not complete host specificity. However, laboratory tests are necessarily artificial, in which many host finding and acceptance cues may be circumvented. We offered sentinel egg masses of three native pentatomid (Hemiptera: Pentatomidae) pest species (Chinavia hilaris (Say), Euschistus conspersus Uhler, and Chlorochroa ligata (Say)) in a field paired-host assay in an area with a well-established adventive population of T. japonicus near Vancouver, WA. Overall, 67% of the H. halys egg masses were parasitized by T. japonicus during the 2-yr study. Despite the ‘worst case’ scenario for a field test (close proximity of the paired egg masses), the rate of parasitism (% eggs producing adult wasps) on all three native species was significantly less (0.4–8%) than that on H. halys eggs (77%). The levels of successful parasitism of T. japonicus of the three species are C. hilaris > E. conspersus > C. ligata. The potential impact of T. japonicus on these pentatomids is probably minimal.


Nematology ◽  
2001 ◽  
Vol 3 (5) ◽  
pp. 463-472 ◽  
Author(s):  
John Kirkegaard ◽  
Rod McLeod ◽  
Christopher Steel

AbstractInvasion, development and egg laying by Meloidogyne javanica in 11 Brassicaceae and four non-Brassicaceae crop species/subspecies was investigated. At 10 to15 and 15 to 20°C, fodder rape cv. Rangi was invaded less than the good hosts tomato cv. Grosse Lisse and field pea cv. Dun but more than the poor host oat cv. Cooba. With an inoculum of 50 second stage juveniles (J2), invasion of Rangi, and the intermediate host subterranean clover cv. Trikkala, were similarly invaded when inoculated with 50 and 100 J2, cv. Rangi was invaded less than tomato. The intermediate host subterranean clover cv. Trikkala and Rangi were similarly invaded when inoculated with 50 and 100 J2 but cv. Trikkala was less invaded with 200 J2. Oat cv. Cooba was always less invaded than the other hosts. Invasion of 3-week-old seedlings of cv. Rangi and 12 cultivars of seven other Brassicaceae crop species/subspecies were similar. Three weeks after inoculation, more M. javanica had developed to the mature female stage in tomato than in the eight Brassicaceae species/subspecies. Females growing in tomato and field pea were always larger than those in rape cv. Rangi. Females in Rangi were larger but those in oilseed radish cv. Adagio were smaller than in 11 other cultivars of seven Brassicaceae, except in plants grown in winter. Egg masses from four Brassicaceae species contained fewer eggs than egg masses from tomato at 6 weeks after inoculation, but at 7 and 8 weeks only those from fodder rape cv. Korina had consistently fewer than tomato. Results are discussed in relation to host status, glucosinolates and potential use of Brassicaceae for control of Meloidogyne.


2006 ◽  
Vol 31 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Susan I Barr

Dietary Reference Intakes (DRIs) are nutrient reference standards used for planning and assessing the diets of apparently healthy Canadians and Americans. The development of DRIs reflects a joint initiative by the United States and Canada to update, expand on, and replace the former Recommended Nutrient Intakes for Canadians and Recommended Dietary Allowances for Americans. DRIs include the Estimated Average Requirement (EAR), Recommended Dietary Allowance (RDA), Adequate Intake (AI), and Tolerable Upper Intake Level (UL). The EAR is the average daily intake level that meets the requirement of 50% of healthy individuals in a life stage and gender group, whereas the RDA is set at a level that will meet the requirements of almost all (97%-98%) individuals in that life stage and gender group. An AI is a recommended intake level that is thought to meet the needs of almost all healthy individuals, and is set when there are insufficient data to establish an EAR (and therefore an RDA). The UL represents a threshold above which adverse effects of excessive intake may increase. In addition to these DRIs, macro nutrients have an Acceptable Macro nutrient Distribution Range (AMDR) and, for energy, an Estimated Energy Requirement (EER) is described.Key words: nutrient reference standards, nutrient requirements, nutrient toxicity.


Plant Disease ◽  
2001 ◽  
Vol 85 (9) ◽  
pp. 1030-1030 ◽  
Author(s):  
S. H. Thomas ◽  
J. M. Fuchs ◽  
Z. A. Handoo

For several years, decline was observed in mature pecan (Carya illinoensis (F.A. Wangenheim) K. Koch) trees in an orchard in Dona Ana County, New Mexico despite normal fertilization and irrigation practices. Affected trees were growing in sandy soil in two widely separated irrigation terraces and exhibited chlorosis of foliage and substantial die-back of branches in the upper canopy. Examination of feeder roots revealed the presence of numerous small galls and egg masses, with root-knot nematode females often visibly protruding from root tissue. Attempts to culture the nematode on tomato (Lycopersicon esculentum Mill. ‘Rutgers’) were unsuccessful. Females and egg masses were collected from fresh pecan roots and sent to the USDA Nematology Laboratory in Beltsville, MD, in October 2000, where specimens were identified as Meloidogyne partityla Kleynhans (1) based on morphological examination. This is the first report of M. partityla from New Mexico, and the second report of this nematode outside South Africa. Starr et al. (2) first reported M. partityla from pecan in the United States in 1996, after recovering the nematode from five orchards in Texas. In their study, the host range of M. partityla was limited to members of the Juglandaceae, which may explain the inability of the New Mexico population to reproduce on tomato. Additional information is needed regarding distribution of this nematode within pecan-growing regions throughout North America. References: (1) K. P. N. Kleynhans. Phytophylactica 18:103, 1986. (2) J. L. Starr et al. J. Nematol. 28:565, 1996.


2007 ◽  
Vol 2 (2) ◽  
pp. 97
Author(s):  
Wendy Furlan

A review of: Shachaf, Pnina, and Sarah Horowitz. "Are Virtual Reference Services Color Blind?" Library & Information Science Research 28.4 (Sept. 2006): 501-20. Abstract Objective – To examine whether librarians provide equitable virtual reference services to diverse user groups. Design – Unobtrusive method of defined scenarios submitted via e-mail. Setting – Twenty-three Association of Research Libraries (ARL) member libraries from across the United States. All ARL member libraries were invited to participate, with the 23 acceptances providing 19% participation. Subjects – Anonymous librarians from the 23 participating libraries’ virtual e-mail reference services. Up to 6 librarians from each library may have been involved. Six fictitious personas were developed to represent particular ethnic or religious groups, whereby the ethnic or religious affiliation was only indicated by the name chosen for each user and the corresponding e-mail address. Names were selected from lists of names or baby names available online: Latoya Johnson (African-American), Rosa Manuz (Hispanic), Chang Su (Asian - Chinese), Mary Anderson (Caucasian/Christian), Ahmed Ibrahim (Muslim), and Moshe Cohen (Caucasian/Jewish). These personas were used to submit reference queries via e-mail to the virtual reference services taking part in the study. Methods – Five different types of reference queries were developed for use in this study. Three were based on prior published research as they were deemed to be answerable by the majority of libraries. They included a dissertation query, a sports team query, and a population query all designed to be tailored to the target institution. The other 2 queries were developed with participating institutions’ virtual reference guidelines in mind, and were thought to not be answered by the target institutions when submitted by unaffiliated users. They consisted of a subject query on a special collection topic that asked for copies of relevant articles to be sent out, and an article query requesting that a copy of a specific article be e-mailed to the patron. The study was conducted over a 6 week period beginning the second week of September, 2005. Each week, 1 fictitious persona was used to e-mail a reference query to the virtual reference service of each of the 23 participating institutions. Five of each type of query were sent by each persona. During September and October 2005, a total of 138 queries were sent. Each institution received a different query for each of the first 5 weeks, and in the sixth week they received a repeat of a previous request with details of title or years altered. All other text in every request sent was kept consistent. Each institution only received 1 request from each persona during the study. In order to eliminate any study bias caused by an informed decision regarding the order in which personas were used, they were randomly arranged (alphabetically by surname). Furthermore, to avoid suspicions from responding librarians, queries were e-mailed on different days of the week at different times. This created some limitations in interpretating response times as some queries were submitted on weekends. All queries were analysed by Nvivo software in order to identify attributes and patterns to aid qualitative analysis. Each transaction (a single query and any related responses) was classified according to 12 attributes and 59 categories based on various associations’ digital reference guidelines. Transactions were coded and then 10% re-coded by a different coder. This led to the clarification and refinement of the coding scheme, resulting in the number of categories used being reduced to 23. Coding was then performed in 3 iterations until 90% agreement between the 2 coders was reached. The final inter-coder reliability was 92%. The study did not support cross tabulation among user groups on most content categories due to the small sample size. Main results – Response times varied greatly between users. Moshe (Caucasian/Jewish) received an average turn-around of less than a day. At the other end of the spectrum, Ahmed’s (Muslim) responses took an average of 3.5 days. Both Ahmed and Latoya (African-American) sent queries which took over 18 days to receive a response. The length (number of words) of replies also indicated a differing level of service with Mary (Caucasian/Christian) and Moshe receiving far lengthier responses than the other 4 personas. Number of replies (including automatic replies) was examined in comparison with the number of replies which answered the question, and again indicated Mary and Moshe were receiving a better level of service. The way in which the user was addressed by the librarian was examined as another measure of service, i.e. first name, full name, honorific. This again mirrored the low level of service received by Ahmed. The professional endings used by librarians in their replies also reinforced the high quality of service received by Moshe across other categories. Results for Rosa (Latino) and Chang (Asian - Chinese) were average for most categories presented. Conclusion – In this study, a discriminatory pattern was clearly evident, with the African-American and Muslim users receiving poor levels of service from virtual reference librarians across all dimensions of quality evaluated. The Caucasian (Christian and Jewish) users also noticeably received the best level of service. It is noted, however, that the sample size of the study is not large enough for generalisations to be drawn and that future, more statistically significant studies are warranted. Many other questions are raised by the study for possible future research into racism exhibited by library staff and services.


2020 ◽  
Author(s):  
Danielle M. Tufts ◽  
Laura B. Goodman ◽  
Meghan C. Benedict ◽  
April D. Davis ◽  
Meredith C. VanAcker ◽  
...  

AbstractHaemaphysalis longicornis, the Asian longhorned tick, is an invasive ixodid tick that has rapidly spread across the northeastern and southeastern regions of the United States since first reported in 2017. The emergence of H. longicornis presents a potential threat for livestock, wildlife, and human health as the host associations and vector potential of this invasive pest in the United States are poorly understood. Previous field data from the United States has shown that H. longicornis was not associated with natural populations of small mammals or birds, but they show a preference for medium sized mammals in laboratory experiments. Therefore, medium and large sized mammals were sampled on Staten Island, New York to determine H. longicornis host associations and vector potential for a range of human and veterinary pathogens. A total of 97 hosts were sampled and five species of tick (Amblyomma americanum, Dermacentor variabilis, H. longicornis, Ixodes scapularis, Ixodes cookei) were found feeding concurrently on these hosts. Haemaphysalis longicornis was found in the highest proportions compared to other native tick species on raccoons (55.4%), Virginia opossums (28.9%), and white-tailed deer (11.5%). Tissue, blood, and engorged larvae were tested for 17 different pathogens using a nanoscale PCR platform. Infection with five pathogens (Borrelia burgdorferi, Anaplasma phagocytophilum, Rickettsia spp., Mycoplasma haemocanis, and Bartonella spp.) was detected in host samples, but no pathogens were found in any larval samples. These results suggest that although large and medium sized mammals feed large numbers of H. longicornis ticks in the environment there is presently a low potential for H. longicornis to acquire pathogens from these wildlife hosts.HighlightsH. longicornis were sampled from seven genera of large and medium-sized mammalsRaccoons, opossums, and white-tailed deer fed a large proportion of H. longicornisH. longicornis did not acquire pathogens through co-feeding with native tick vectorsHost species were infected with a range of pathogens of human and veterinary concernHost-derived H. longicornis engorged larvae were not infected with any pathogens


2015 ◽  
Vol 78 (7) ◽  
pp. 1370-1374
Author(s):  
ANDREAS KIERMEIER ◽  
JOHN SUMNER ◽  
IAN JENSON

Australia exports about 150,000 to 200,000 tons of manufacturing beef to the United States annually. Each lot is tested for Escherichia coli O157 using the N-60 sampling protocol, where 60 small pieces of surface meat from each lot of production are tested. A risk assessment of E. coli O157 illness from the consumption of hamburgers made from Australian manufacturing meat formed the basis to evaluate the effect of sample size and amount on the number of illnesses predicted. The sampling plans evaluated included no sampling (resulting in an estimated 55.2 illnesses per annum), the current N-60 plan (50.2 illnesses), N-90 (49.6 illnesses), N-120 (48.4 illnesses), and a more stringent N-60 sampling plan taking five 25-g samples from each of 12 cartons (47.4 illnesses per annum). While sampling may detect some highly contaminated lots, it does not guarantee that all such lots are removed from commerce. It is concluded that increasing the sample size or sample amount from the current N-60 plan would have a very small public health effect.


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