scholarly journals Determination of the Metabolite of Ephedrine, 4-Hydroxyephedrine, by LC–MS-MS in Rat Urine and Its Application in Excretion Profiles After Oral Administration ofEphedra sinica Stapfand ProcessingEphedra sinica Stapf

2016 ◽  
Vol 55 (2) ◽  
pp. 162-165 ◽  
Author(s):  
Jie Xu ◽  
Rui Yan
Keyword(s):  
Oral Administration ◽  
Rat Urine

Simultaneous Determination of Triterpenoidal Saponins in Rat Urine After Oral Administration of Pulsatilla chinensis Extract Using HPLC–MS

2015 ◽  
Vol 78 (3-4) ◽  
pp. 231-239 ◽  
Author(s):  
Miao Miao Jin ◽  
Geng Shen Song ◽  
Ying Feng Du ◽  
Liang Cao ◽  
Hui Jun Xu ◽  
...  
Keyword(s):  
Oral Administration ◽  
Simultaneous Determination ◽  
Rat Urine ◽  
Pulsatilla Chinensis

scholarly journals Comparative Study on Excretive Characterization of Main Components in Herb Pair Notoginseng-Safflower and Single Herbs by LC–MS/MS

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 241 ◽  
Author(s):  
Ying-Yuan Lu ◽  
Jin-Yang Song ◽  
Yan Li ◽  
Yu-Qing Meng ◽  
Ming-Bo Zhao ◽  
...  
Keyword(s):  
Oral Administration ◽  
Gradient Elution ◽  
Fecal Excretion ◽  
Bioactive Constituents ◽  
Rat Urine ◽  
Liquid Chromatography Tandem Mass ◽  
Main Components ◽  
Cumulative Excretion

The herbal medicine combination of notoginseng-safflower has been commonly used clinically for the prevention and treatment of cardiovascular diseases. A reliable liquid chromatography-tandem mass spectrometry (LC–MS/MS) method was developed for simultaneous determination of six bioactive components (hydroxysafflor yellow A, notoginsenoide R1, ginsenoside Rb1, Re, Rd, and Rg1) in rat urine and feces after oral administration of notoginseng total saponins (NS), safflower total flavonoids (SF), and the combination of NS and SF (CNS). The chromatographic separation was achieved on a Waters HSS T3 column under gradient elution with acetonitrile and water containing formic acid as the mobile phase. The calibration curves were linear, with correlation coefficient (r) > 0.99 for six components. The intra- and interday precision (RSD) and accuracy (RE) of QC samples were within −14.9% and 14.9%, respectively. The method was successfully applied to study of the urinary and fecal excretion of six bioactive constituents following oral administration of NS, SF, and CNS in rats. Compared to the single herb, the cumulative excretion ratios of six constituents were decreased in the herbal combination. The study indicated that the combination of notoginseng and safflower could reduce the renal and fecal excretion of the major bioactive constituents and promote their absorption in rats.

HPLC determination of four triterpenoids in rat urine after oral administration of total triterpenoids from Ganoderma lucidum

2007 ◽  
Vol 43 (3) ◽  
pp. 1185-1190 ◽  
Author(s):  
Xiao-Ming Wang ◽  
Shu-Hong Guan ◽  
Rong-Xia Liu ◽  
Jiang-Hao Sun ◽  
Yan Liang ◽  
...  
Keyword(s):  
Oral Administration ◽  
Ganoderma Lucidum ◽  
Hplc Determination ◽  
Rat Urine

Simultaneous Determination of Imatinib and N-Desmethyl Imatinib in Rat Plasma and Tissues Using LC-MS/MS

2019 ◽  
Vol 15 (2) ◽  
pp. 121-129
Author(s):  
Zhi Rao ◽  
Bo-xia Li ◽  
Yong-Wen Jin ◽  
Wen-Kou ◽  
Yan-rong Ma ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Oral Administration ◽  
Parent Drug ◽  
Gradient Elution ◽  
Biological Tissues ◽  
Rat Plasma ◽  
Running Water ◽  
Liver And Kidney ◽  
The Brain

Background: Imatinib (IM) is a chemotherapy medication metabolized by CYP3A4 to Ndesmethyl imatinib (NDI), which shows similar pharmacologic activity to the parent drug. Although methods for determination of IM and/or NDI have been developed extensively, only few observations have been addressed to simultaneously determine IM and NDI in biological tissues such as liver, kidney, heart, brain and bone marrow. Methods: A validated LC-MS/MS method was developed for the quantitative determination of imatinib (IM) and N-desmethyl imatinib (NDI) from rat plasma, bone marrow, brain, heart, liver and kidney. The plasma samples were prepared by protein precipitation, and then the separation of the analytes was achieved using an Agilent Zorbax Eclipse Plus C18 column (4.6 × 100 mm, 3.5 µm) with gradient elution running water (A) and methanol (B). Mass spectrometric detection was achieved by a triplequadrupole mass spectrometer equipped with an electrospray source interface in positive ionization mode. Results: This method was used to investigate the pharmacokinetics and the tissue distributions in rats following oral administration of 25 mg/kg of IM. The pharmacokinetic profiles suggested that IM and NDI are disappeared faster in rats than human, and the tissue distribution results showed that IM and NDI had good tissue penetration and distribution, except for the brain. This is the first report about the large penetrations of IM and NDI in rat bone marrow. Conclusion: The method demonstrated good sensitivity, accuracy, precision and recovery in assays of IM and NDI in rats. The described assay was successfully applied for the evaluation of pharmacokinetics and distribution in the brain, heart, liver, kidney and bone marrow of IM and NDI after a single oral administration of IM to rats.

Simultaneous determination of arctiin and its metabolites in rat urine and feces by HPLC

Fitoterapia ◽  
2013 ◽  
Vol 86 ◽  
pp. 6-12 ◽  
Author(s):  
Wei Wang ◽  
Qiang Pan ◽  
Xue-Ying Han ◽  
Jing Wang ◽  
Ri-Qiu Tan ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Rat Urine
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