HPLC determination of four triterpenoids in rat urine after oral administration of total triterpenoids from Ganoderma lucidum

2007 ◽  
Vol 43 (3) ◽  
pp. 1185-1190 ◽  
Author(s):  
Xiao-Ming Wang ◽  
Shu-Hong Guan ◽  
Rong-Xia Liu ◽  
Jiang-Hao Sun ◽  
Yan Liang ◽  
...  
Keyword(s):  
Oral Administration ◽  
Ganoderma Lucidum ◽  
Hplc Determination ◽  
Rat Urine

Simultaneous Determination of Triterpenoidal Saponins in Rat Urine After Oral Administration of Pulsatilla chinensis Extract Using HPLC–MS

2015 ◽  
Vol 78 (3-4) ◽  
pp. 231-239 ◽  
Author(s):  
Miao Miao Jin ◽  
Geng Shen Song ◽  
Ying Feng Du ◽  
Liang Cao ◽  
Hui Jun Xu ◽  
...  
Keyword(s):  
Oral Administration ◽  
Simultaneous Determination ◽  
Rat Urine ◽  
Pulsatilla Chinensis

scholarly journals Comparative Study on Excretive Characterization of Main Components in Herb Pair Notoginseng-Safflower and Single Herbs by LC–MS/MS

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 241 ◽  
Author(s):  
Ying-Yuan Lu ◽  
Jin-Yang Song ◽  
Yan Li ◽  
Yu-Qing Meng ◽  
Ming-Bo Zhao ◽  
...  
Keyword(s):  
Oral Administration ◽  
Gradient Elution ◽  
Fecal Excretion ◽  
Bioactive Constituents ◽  
Rat Urine ◽  
Liquid Chromatography Tandem Mass ◽  
Main Components ◽  
Cumulative Excretion

The herbal medicine combination of notoginseng-safflower has been commonly used clinically for the prevention and treatment of cardiovascular diseases. A reliable liquid chromatography-tandem mass spectrometry (LC–MS/MS) method was developed for simultaneous determination of six bioactive components (hydroxysafflor yellow A, notoginsenoide R1, ginsenoside Rb1, Re, Rd, and Rg1) in rat urine and feces after oral administration of notoginseng total saponins (NS), safflower total flavonoids (SF), and the combination of NS and SF (CNS). The chromatographic separation was achieved on a Waters HSS T3 column under gradient elution with acetonitrile and water containing formic acid as the mobile phase. The calibration curves were linear, with correlation coefficient (r) > 0.99 for six components. The intra- and interday precision (RSD) and accuracy (RE) of QC samples were within −14.9% and 14.9%, respectively. The method was successfully applied to study of the urinary and fecal excretion of six bioactive constituents following oral administration of NS, SF, and CNS in rats. Compared to the single herb, the cumulative excretion ratios of six constituents were decreased in the herbal combination. The study indicated that the combination of notoginseng and safflower could reduce the renal and fecal excretion of the major bioactive constituents and promote their absorption in rats.

Sign in / Sign up

Export Citation Format

Share Document